3D bio-printed biphasic scaffolds with dual modification of silk fibroin for the integrated repair of osteochondral defects

Deng, Changxu; Jin, Yang; He, Hongtao; Ma, Zhen; Wang, Wenhao; Zhang, Yuxin; Li, Tao; He, Chuanglong; Wang, Jinwu

doi:10.1039/d1bm00535a

Cited by 47 publications

(54 citation statements)

References 51 publications

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“…Methacrylated SF (SFMA) was synthesized as described previously [ 25 ]. Briefly, 40 g of silkworm cocoon was boiled in 2 L of 2% Na 2 CO 3 solution for 30 min at 100 °C and then washed several times with distilled water.…”

Section: Methodsmentioning

confidence: 99%

Decoding the annulus fibrosus cell atlas by scRNA-seq to develop an inducible composite hydrogel: A novel strategy for disc reconstruction

Han

Wang

Luo

et al. 2022

Bioactive Materials

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Section: Methodsmentioning

confidence: 99%

Decoding the annulus fibrosus cell atlas by scRNA-seq to develop an inducible composite hydrogel: A novel strategy for disc reconstruction

Han

Wang

Luo

et al. 2022

Bioactive Materials

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“…Previous studies have suggested that parathyroid hormone (PTH) inhibits chondrocyte hypertrophy and facilitates articular hyaline chondrogenesis. Deng et al (2021) used silk fibroin (SF) grafted with PTH by sulfonated SMCC (SF-PTH), covalently immobilized methacrylic anhydride (SF-MA), and photo-crosslinkable gelatin methacryloyl (GMA) for gradient strengthening of the scaffold based on natural mechanical strength. BMSCs were co-cultured separately in vitro with four bioinks (10% GM, 10% GM-5% SF, 10% GM-5% SF-MA, and 10% GM-5% SF-PTH).…”

Section: 3d Printingmentioning

confidence: 99%

“…All four bioinks had good biocompatibility, while GM + SF-PTH ink inhibited the hypertrophy of culutred chondrocytes. After implantation of GM + SF-PTH/GM + SF-MA scaffolds in rabbit distal femoral talar sulcus defects, higher macroscopic scores, and fewer specific markers of chondrocyte hypertrophy, were observed compared with controls, demonstrating that this mechanically graded bioprinted biphasic scaffold can effectively promote regeneration of osteochondral defects, while PTH helps maintain the phenotype of hyaline cartilage ( Deng et al, 2021 ).…”

Section: 3d Printingmentioning

confidence: 99%

3D Printing for Bone-Cartilage Interface Regeneration

Jiao

et al. 2022

Front. Bioeng. Biotechnol.

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Due to the vasculature defects and/or the avascular nature of cartilage, as well as the complex gradients for bone-cartilage interface regeneration and the layered zonal architecture, self-repair of cartilage and subchondral bone is challenging. Currently, the primary osteochondral defect treatment strategies, including artificial joint replacement and autologous and allogeneic bone graft, are limited by their ability to simply repair, rather than induce regeneration of tissues. Meanwhile, over the past two decades, three-dimension (3D) printing technology has achieved admirable advancements in bone and cartilage reconstruction, providing a new strategy for restoring joint function. The advantages of 3D printing hybrid materials include rapid and accurate molding, as well as personalized therapy. However, certain challenges also exist. For instance, 3D printing technology for osteochondral reconstruction must simulate the histological structure of cartilage and subchondral bone, thus, it is necessary to determine the optimal bioink concentrations to maintain mechanical strength and cell viability, while also identifying biomaterials with dual bioactivities capable of simultaneously regenerating cartilage. The study showed that the regeneration of bone-cartilage interface is crucial for the repair of osteochondral defect. In this review, we focus on the significant progress and application of 3D printing technology for bone-cartilage interface regeneration, while also expounding the potential prospects for 3D printing technology and highlighting some of the most significant challenges currently facing this field.

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“…Radial and circumferential (both outer and inner layers) tensile modulus readings showed that implants lagged behind native cartilage by margins of less than 5%. By contrast, Deng et al employed human parathyroid hormone (PH) to prevent chondrocyte hypertrophy in order to achieve and maintain hyaline-like properties (Deng, 2021). They achieved this using a biphasic, layered 3D bioprinted construct made of two distinct bioinks.…”

Section: Cell-based Bioprinting Of Knee Cartilagementioning

confidence: 99%

3D Bioprinted Implants for Cartilage Repair in Intervertebral Discs and Knee Menisci

Perera

Ivone

Natekin

et al. 2021

Front. Bioeng. Biotechnol.

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Cartilage defects pose a significant clinical challenge as they can lead to joint pain, swelling and stiffness, which reduces mobility and function thereby significantly affecting the quality of life of patients. More than 250,000 cartilage repair surgeries are performed in the United States every year. The current gold standard is the treatment of focal cartilage defects and bone damage with nonflexible metal or plastic prosthetics. However, these prosthetics are often made from hard and stiff materials that limits mobility and flexibility, and results in leaching of metal particles into the body, degeneration of adjacent soft bone tissues and possible failure of the implant with time. As a result, the patients may require revision surgeries to replace the worn implants or adjacent vertebrae. More recently, autograft – and allograft-based repair strategies have been studied, however these too are limited by donor site morbidity and the limited availability of tissues for surgery. There has been increasing interest in the past two decades in the area of cartilage tissue engineering where methods like 3D bioprinting may be implemented to generate functional constructs using a combination of cells, growth factors (GF) and biocompatible materials. 3D bioprinting allows for the modulation of mechanical properties of the developed constructs to maintain the required flexibility following implantation while also providing the stiffness needed to support body weight. In this review, we will provide a comprehensive overview of current advances in 3D bioprinting for cartilage tissue engineering for knee menisci and intervertebral disc repair. We will also discuss promising medical-grade materials and techniques that can be used for printing, and the future outlook of this emerging field.

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3D bio-printed biphasic scaffolds with dual modification of silk fibroin for the integrated repair of osteochondral defects

Abstract: Repair of osteochondral defects is still a challenge, especially the regeneration of hyaline cartilage. Parathyroid hormone (PTH) can inhibit the hypertrophy of chondrocytes to maintain the phenotype of hyaline...

Cited by 47 publications

References 51 publications

Decoding the annulus fibrosus cell atlas by scRNA-seq to develop an inducible composite hydrogel: A novel strategy for disc reconstruction

Decoding the annulus fibrosus cell atlas by scRNA-seq to develop an inducible composite hydrogel: A novel strategy for disc reconstruction

3D Printing for Bone-Cartilage Interface Regeneration

3D Bioprinted Implants for Cartilage Repair in Intervertebral Discs and Knee Menisci

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