3D additive-manufactured nanocomposite magnetic scaffolds: Effect of the application mode of a time-dependent magnetic field on hMSCs behavior

Abstract

Over the past few years, the influence of static or dynamic magnetic fields on biological systems has become a topic of considerable interest. Magnetism has recently been implicated to play significant roles in the regulation of cell responses and, for this reason, it is revolutionizing many aspects of healthcare, also suggesting new opportunities in tissue engineering.The aim of the present study was to analyze the effect of the application mode of a time-dependent magnetic field on the behavior of human mesenchymal stem cells (hMSCs) seeded on 3D additive-manufactured poly(ɛ-caprolactone)/iron-doped hydroxyapatite (PCL/FeHA) nanocomposite scaffolds.

Bioactive chitosan‐based scaffolds with improved properties induced by dextran‐grafted nano‐maghemite and l‐arginine amino acid

“…After 30 min of incubation, culture medium was added to each well containing one cell‐laden scaffold. At 1 day from cell seeding, some cell‐laden scaffolds were stimulated, in a magnetic bioreactor, using a discontinuous application of a time‐dependent magnetic field (70 Hz and intensity of 25–30 mT), for 6 h per day (20 intervals – 18 min each). Further cell‐laden scaffolds, which were not magnetically stimulated, were used as control and placed in the same incubator.…”

“…Cytocompatibility in the presence of magnetic stimulation. Undifferentiated human mesenchymal stem cells (hMSCs; Clonetics, Italy), at the fourth passage, were cultured in α-MEM (Bio-Whittaker, Belgium) containing 10% v/v FBS (GibcoTM, Thermo Fisher Scientific), 100 U/mL penicillin and 0.1 mg/mL streptomycin (HyClone, UK), in a humidified 18 in a magnetic bioreactor, using a discontinuous application of a time-dependent magnetic field (70 Hz and intensity of 25-30 mT), for 6 h per day (20 intervals -18 min each). Further cell-laden scaffolds, which were not magnetically stimulated, were used as control and placed in the same incubator.…”

“…MNPs‐incorporating biomaterials have been shown to have high potential in activating cells involved in osteogenic processes, being responsive to external magnetic fields, which affect the cellular microenvironment . The static or alternating magnetic stimulation has been highlighted to promote the implant integration and increase the newly developed bone density, thus promoting a rapid and better healing of the injury . Static magnetic fields (1 mT to 1 T) have shown to accelerate the proliferation, migration, orientation and differentiation of osteoblast‐like cells .…”

“…17 The static or alternating magnetic stimulation has been highlighted to promote the implant integration and increase the newly developed bone density, thus promoting a rapid and better healing of the injury. 18,19 Static magnetic fields (1 mT to 1 T) have shown to accelerate the proliferation, migration, orientation and differentiation of osteoblastlike cells. [20][21][22] Moreover, it has been proven to induce the osteogenic differentiation in bone marrow-derived mesenchymal stem cells 23 as a consequence of diamagnetic properties of cell membranes and extracellular matrices, 24 which modify their fluxes, and structures according to static magnetic stimuli.…”

Advances in Magnetic Nanoparticles for Biomedical Applications

“…This can be overcome by the application of a mechanical stimulation based on a biomagnetic approach (Figure c). Different strategies based on biomagnetic biomaterial were developed, such as films, hydrogels, fibers, and 3D scaffolds . The combination of magnetic particles with polymeric biomaterials has demonstrated large potential for disease treatment and tissue repair, namely bone, muscle, nerve and cardiac tissue regeneration with external magnetic stimulation.…”