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Nanthakumar, T.; Kataria, R. S.; Tiwari, Ashok Kumar; Butchaiah, G.; Kataria, J.M.

doi:10.1023/a:1006403017578

Cited by 88 publications

(20 citation statements)

References 14 publications

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“…Previously, several researchers reported methods for differentiating avirulent and virulent APMV-1 strains by simple REA using Bgl I [4,11]. We applied these methods to the APMV-1 strains used in this study, but the VG/GA vaccine strain was misidentified as a virulent strain (data not shown), as also suggested in a review by Cattoli [2].…”

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confidence: 78%

Simple Differentiation of Avirulent and Virulent Strains of Avian Paramyxovirus Serotype-1 (Newcastle Disease Virus) by PCR and Restriction Endonuclease Analysis in Japan

Mase

Kanehira

2012

The Journal of Veterinary Medical Science

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ABSTRACT. To differentiate the avirulent from virulent strains of avian paramyxovirus serotype-1 (APMV-1, Newcastle disease virus), PCR and restriction endonuclease analysis (REA) was employed. Primer sequences were used to amplify a 766-base pair fragment that included the fusion protein cleavage site. REA of PCR products generated by Hin1 I and Apa I enabled the differentiation of avirulent field and vaccine strains from more virulent field strains of APMV-1 in Japan.

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confidence: 78%

Simple Differentiation of Avirulent and Virulent Strains of Avian Paramyxovirus Serotype-1 (Newcastle Disease Virus) by PCR and Restriction Endonuclease Analysis in Japan

Mase

Kanehira

2012

The Journal of Veterinary Medical Science

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“…The RT-PCR amplification of the partial fusion protein gene of the NDV revealed 1148 bp product for all the samples (Figure-6). RT-PCR technique has been routinely used for diagnosis of NDV and detecting NDV in clinical samples without necessitating virus isolation in ECE [13,14]. Singh et al .…”

Section: Resultsmentioning

confidence: 99%

Molecular characterization of velogenic viscerotropic Ranikhet (Newcastle) disease virus from different outbreaks in desi chickens

Dhaygude

Sawale

Chawak³

et al. 2017

Vet World

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Aim:Diagnosis of velogenic viscerotropic Ranikhet disease from six different flocks of desi chicken in and around Mumbai by gross and histopathological examination, isolation of virus and molecular methods.Materials and Methods:A total of 25 carcasses (varying between 2 and 6 carcasses from each flock) of six different flocks of adult desi chicken were subjected to necropsy examination for diagnosis of the disease during the span of a year (2014-2015). After thorough gross examination, the tissue samples were collected and processed for virus isolation and histopathological examination. The 20% tissue homogenate was inoculated into 9-day-old specific pathogen free (SPF) embryonated eggs. Mean death time (MDT) of embryos after inoculation and intracerebral pathogenicity index (ICPI) were used to judge velogenic nature of the virus. Newcastle disease virus (NDV) was isolated from six cases and confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) targeting the partial fusion protein gene of the viral genome.Results:A total of 25 carcasses (varying between 2 and 6 carcasses from each flock) of six different flocks of desi chicken were presented for postmortem examination to Department of Veterinary Pathology, Bombay Veterinary College, Parel, Mumbai during 2014-2015. The gross and histopathological examination revealed lesions suggestive of viscerotropic velogenic form of the Newcastle disease (ND). The 20% tissue homogenate was inoculated into 9-day-old embryonated eggs from SPF chicken. NDV was isolated from six cases and confirmed by RT-PCR targeting the partial fusion protein gene. MDT of all the isolates was <60 h which indicated velogenic nature of the virus. ICPI of the isolates ranged between the 1.63 and 1.78. In four out of six outbreaks concurrent moderate to heavy infection of Ascardii galli in one flock and Railetina spp. in three flocks was also noted. In this study, viscerotropic velogenic form of ND was confirmed in all six outbreaks by gross and microscopic examination, virus isolation and RT-PCR.Conclusions:In this study, viscerotropic velogenic form of ND was confirmed in all six outbreaks by gross and microscopic examination, virus isolation and RT-PCR. Nowadays, vaccine strains Lasota, B1 and F strains are used widely in India to control the infection of NDV. However, virulent NDV strains are still isolated frequently in the birds under backyard and also in commercial venture which demonstrates that NDV remains an on-going threat to commercial as well as backyard poultry flocks.

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“…Interestingly, a similar aPMV-1 strain was also described in India (UP2-92). This isolate from pigeons was characterised by a mesogenic ICPI but with an F protein cleavage site motif and restriction enzyme patterns typical of apathogenic vaccine strains [22,23]. The isolation of such strains highlights the importance of a better understanding of the virus pathogenesis in pigeons and the role that pigeons might be playing in the appearance or propagation of virulent strains of NDV.…”

Section: Discussionmentioning

confidence: 97%

Quasispecies Nature of an Unusual Avian Paramyxovirus Type-1 Isolated from Pigeons

Barbezange

Jestin

2005

Virus Genes

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An avian paramyxovirus type-1 (APMV-1) was classified as virulent according to its Intra Cerebral Pathogenicity Index (ICPI), but as avirulent according to the motif of its F protein cleavage site. Although this atypical APMV-1 was isolated from sick, unvaccinated pigeons, it was not grouped with pigeon variants regarding its antigenic and genetic characterisation. We analysed its quasispecies nature by cloning and sequencing parts of the genome in three different genes to evaluate if heterogeneity might explain the difference observed between the ICPI and the F protein cleavage site motif. Two distinct sub-populations were detected in the phosphoprotein gene. In the fusion protein gene, two clones were found to be related to typical pigeon variants in the hypervariable domain.

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Cited by 88 publications

References 14 publications

Simple Differentiation of Avirulent and Virulent Strains of Avian Paramyxovirus Serotype-1 (Newcastle Disease Virus) by PCR and Restriction Endonuclease Analysis in Japan

Simple Differentiation of Avirulent and Virulent Strains of Avian Paramyxovirus Serotype-1 (Newcastle Disease Virus) by PCR and Restriction Endonuclease Analysis in Japan

Molecular characterization of velogenic viscerotropic Ranikhet (Newcastle) disease virus from different outbreaks in desi chickens

Quasispecies Nature of an Unusual Avian Paramyxovirus Type-1 Isolated from Pigeons

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