Untitled

Okino, Luci Kimie; Machado, Kátia Maria Gomes; Fabris, Carla; Bononi, Vera Lúcia Ramos

doi:10.1023/a:1008983616033

Cited by 53 publications

(9 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CCB203, Lentinus villosus Klotzsch CCB271 and Psilocybe castanella Peck CCB444 were capable of colonizing the columns of soil with 4600 mg PCP kg )1 soil (Table 1). Although the majority of the isolates studied are lignicolous species (Okino et al 2000), the results showed that many of them developed well in the soil column. Due to the toxic effect of PCP as an inhibitor of oxidative phosphorylation, the growth of these fungi at the higher PCP concentration was unexpected.…”

Section: Resultsmentioning

confidence: 93%

See 1 more Smart Citation

Biodegradation of pentachorophenol by tropical basidiomycetes in soils contaminated with industrial residues

Machado

Matheus

Monteiro

et al. 2005

World J Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 93%

“…The 36 fungal strains studied were previously selected for Remazol brilliant blue R decolorization and a growth rate exceeding 1.0 cm day )1 in a solid medium (Faleiro et al 1996 ;Okino et al 2000). All cultures were from Brazilian ecosystems and were maintained in 2% (w/v) malt extract agar (MEA) at 4°C.…”

Section: Fungimentioning

confidence: 99%

Biodegradation of pentachorophenol by tropical basidiomycetes in soils contaminated with industrial residues

Machado

Matheus

Monteiro

et al. 2005

World J Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

“…Muzariri et al (2001) screened 224 fungal strains from Zimbabwe for ligninolytic and cellulolytic activities. Okino et al (2000) developed a quick screening method and isolated 116 Brazilian tropical rainforest Basidiomycetes expressing respectively, laccase and peroxidase activities. Pela´ez et al (1995) reported that among 68 species isolated from fruit-bodies of Basidiomycetes collected in the central region of Spain, laccase and Mn 2+ -oxidizing peroxidase are more common ligninolytic activities than LiP in the studied conditions.…”

Section: Resultsmentioning

confidence: 99%

Screening for Ligninolytic Enzyme Production by Diverse Fungi from Tunisia

Dhouib

Hamza

Zouari

et al. 2005

World J Microbiol Biotechnol

View full text Add to dashboard Cite

This work represents the first report on the ability of autochthonous fungi from Tunisia to produce ligninolytic enzymes. Three hundred and fifteen fungal strains were isolated from different Tunisian biotopes. These fungal strains were firstly screened on solid media containing Poly R-478 or ABTS as indicator compounds that enabled the detection of lignin-modifying enzymes as specific color reactions. Of the 315 tested strains, 49 exhibited significant ABTS-oxidation activity expressed within the first week of incubation and only 18 strains decolorized the Poly R-478. Liquid cultivations and laccase, manganese peroxidase and lignin peroxidase activity assays of positive strains confirmed that eight efficient enzyme producers were found in the screening. These strains were attributed to the most closely related species using PCR amplification and sequencing of the internal transcribed spacer 'ITS' regions of the ribosomal DNA. The identification results showed fungal genera such as Oxyporus, Stereum and Trichoderma which have been only rarely reported as ligninolytic enzyme producers in the literature. Culture conditions and medium composition were optimized for the laccase producer Trametes trogii CTM 10156. This optimization resulted in high laccase production, 367 times more than in non-optimized conditions and which reached 110 U ml -1 within 15 days of incubation.

show abstract

“…Cellobiase and xylanase activities were detected by observing a clear zone around the colonies after incubation on cellobiose agar (Ventorino et al, 2015) or Luria-Bertani (LB) agar medium (Oxoid, Milan, Italy) supplemented with 0.05% Remazol brilliant blue-R and a 0.5% solution of sonicated xylan (Sigma-Aldrich, Milan, Italy) as described by Ko et al (2012). Ligninase activity was assayed using the culture medium guaiacol agar (CGA) supplemented with 2 g L −1 of alkali lignin (Okino et al, 2000) or 2 g L −1 of vegetable biomass powder from A. donax (Ventorino et al, 2015). Finally, LBM agar medium supplemented with 0.1 g L −1 of Azure-B was used to detect peroxidase activity as previously reported (Ventorino et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

Lignocellulose-Adapted Endo-Cellulase Producing Streptomyces Strains for Bioconversion of Cellulose-Based Materials

et al. 2016

View full text Add to dashboard Cite

Twenty-four Actinobacteria strains, isolated from Arundo donax, Eucalyptus camaldulensis and Populus nigra biomass during natural biodegradation and with potential enzymatic activities specific for the degradation of lignocellulosic materials, were identified by a polyphasic approach. All strains belonged to the genus Streptomyces (S.) and in particular, the most highly represented species was Streptomyces argenteolus representing 50% of strains, while 8 strains were identified as Streptomyces flavogriseus (synonym S. flavovirens) and Streptomyces fimicarius (synonyms Streptomyces acrimycini, Streptomyces baarnensis, Streptomyces caviscabies, and Streptomyces flavofuscus), and the other four strains belonged to the species Streptomyces drozdowiczii, Streptomyces rubrogriseus, Streptomyces albolongus, and Streptomyces ambofaciens. Moreover, all Streptomyces strains, tested for endo and exo-cellulase, cellobiase, xylanase, pectinase, ligninase, peroxidase, and laccase activities using qualitative and semi-quantitative methods on solid growth medium, exhibited multiple enzymatic activities (from three to six). The 24 strains were further screened for endo-cellulase activity in liquid growth medium and the four best endo-cellulase producers (S. argenteolus AE58P, S. argenteolus AE710A, S. argenteolus AE82P, and S. argenteolus AP51A) were subjected to partial characterization and their enzymatic crude extracts adopted to perform saccharification experiments on A. donax pretreated biomass. The degree of cellulose and xylan hydrolysis was evaluated by determining the kinetics of glucose and xylose release during 72 h incubation at 50°C from the pretreated biomass in the presence of cellulose degrading enzymes (cellulase and β-glucosidase) and xylan related activities (xylanase and β-xylosidase). The experiments were carried out utilizing the endo-cellulase activities from the selected S. argenteolus strains supplemented with commercial β-gucosidase and xylanase preparations from Genencore (Accellerase BG and Accellerase XY). Cellulose and xylan conversion, when conducted using commercial (hemi)cellulases, gave glucose and xylose yields of 30.17 and 68.9%, respectively. The replacement of the cellulolytic preparation from Genencor (Accellerase 1500), with the endo-cellulase from S. argenteolus AE58P resulted in almost 76% of the glucose yield obtained in the presence of the commercial counterpart. Due to the promising results obtained by using the enzymatic crude extracts from S. argenteolus AE58P in the pretreated A. donax saccharification experiments, the proteins putatively responsible for endo-cellulase activity in this strain were identified by proteomics. Several proteins were confidently identified in different Streptomyces spp., eight of which belong to the class of Carbohydrate active enzymes. Overall results highlighted the biotechnological potential of S. argenteolus AE58P being an interesting candidate biocatalyst-producing bacterium for lignocellulose conversion and production of biochemicals and bioenergy.

show abstract

Untitled

Cited by 53 publications

References 20 publications

Biodegradation of pentachorophenol by tropical basidiomycetes in soils contaminated with industrial residues

Biodegradation of pentachorophenol by tropical basidiomycetes in soils contaminated with industrial residues

Screening for Ligninolytic Enzyme Production by Diverse Fungi from Tunisia

Lignocellulose-Adapted Endo-Cellulase Producing Streptomyces Strains for Bioconversion of Cellulose-Based Materials

Contact Info

Product

Resources

About