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Barceló, Marta; El-Mansouri, Iman; Mercado, José A.; Quesada, Miguel A.; Alfaro, Fernando Pliego

doi:10.1023/a:1006031527413

Cited by 69 publications

(13 citation statements)

References 31 publications

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“…45,46 In strawberries, dark incubation of leaf explants for at least a week is required to enhance organogenesis in strawberry explants. 9,20,22,[47][48][49][50][51] Elongation of the shoots After shoot development, produced shoots in cluster were transferred to different elongated media and incubated for 4 wks. The most efficient elongation medium was selected upon reaching shoots height to approximately 1 cm.…”

Section: Adventitious Shoots Formationmentioning

confidence: 99%

“…Adventitious buds and shoots regeneration has been previously reported from different explants such as: leaves, [8][9][10][11][12][13][14][15][16] petioles, 11,12,15,17,18 peduncles/peduncular base of the flower buds, 17,18 stems, 19 stipules, 11,20 stolon, 18 roots, 11,20 runners, 9 and anther cultures. 21 Furthermore, shoot regeneration was produced directly from field, 10 greenhouse-grown strawberry plants, 12,15,22 or from in vitro-grown shoots. 1,11 Transformation in strawberries has been previously documented using different constructs in various genotypes.…”

Section: Introductionmentioning

confidence: 99%

“…The transformation of Fragaria was focused on establishing the system and using genes with potential for pest and herbicide resistance, cold tolerance, and ripening. 22,[28][29][30][31][32][33][34][35][36] The aim of this study was to develop an efficient and reproducible Agrobacterium-mediated protocol for the strawberry cultivars using the juvenile leaf explants.…”

Section: Introductionmentioning

confidence: 99%

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Improved regeneration and transformation protocols for three strawberry cultivars

et al. 2013

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strawberry (Fragaria × ananassa) is an economically important soft fruit crop with polyploid genome, which makes the breeding of new cultivars difficult. simple and efficient methods for transformation and regeneration are required for cultivars improvement in strawberries. In the present study, adventitious shoot regeneration has been investigated in three cultivated strawberry plants, i.e., Festival, sweet charlie, and Florida via direct organogenesis using the in vitro juvenile leaves as explants. explants were collected after sub-culturing on a propagation medium composed of Ms supplemented with 0.5 mg/l Ba; 0.1 mg/l Ga3 and 0.1 mg/l IBa. To select the suitable organogenesis, the explants of the three cultivars were cultured on Ms medium supplemented with different concentrations of TDZ (1, 2, 3, and 4 mg/l), then incubated at a temperature of 22 °c ± 2. Medium containing 2 mg/l TDZ revealed the best regeneration efficiency with the three cultivars (72% for Festival and 73% for sweet charlie and Florida). after 4 weeks the produced shoots were cultured on Ms medium with different concentrations of Ba and Kin to enhance shoot elongation. Results showed that the medium containing 1.5 mg/l Ba and 0.5 mg/l Kin revealed highest elongation efficiency (88% and 94%) for Festival and sweet charlie, respectively. On the other hand, medium media containing 1.5 mg/l Ba and 0.1 mg/l Kin showed highest elongation efficiency (90%) in Florida. elongated shoots were successfully rooted on Ms medium containing 1.5 mg/l Naa. Furthermore, transformation of the two cultivars, Festival and sweet charlie, has been established via Agrobacterium strain LBa44404 containing the plasmid pIsV2678 with gus-intron and bar genes. Three days post-cocultivation, GUs activity was screened using the histochemical assay. The results showed 16% and 18% of the tested plant materials had changed into a blue color for Festival and sweet charlie, respectively. Out of 120 explants only 13 shoots were developed on bialaphos medium for each cultivar, representing 10.8% bialaphos-resistant strawberry shoot. The presence of both the genes bar and uid a was detected by PcR and Northern, giving a transformation efficiency of 5%.

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Section: Adventitious Shoots Formationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Improved regeneration and transformation protocols for three strawberry cultivars

et al. 2013

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“…cv. Chandler), micropropagated in a N30K medium supplemented with 2.2 mM kinetin [66], were treated with gibberellic acid. Two groups of six independent clones were grown in MS medium supplemented with GA 3 (100 μM) and PBZ (100 μM), respectively, and kept in a growth chamber for 11 days prior harvest.…”

Section: Methodsmentioning

confidence: 99%

An atypical HLH transcriptional regulator plays a novel and important role in strawberry ripened receptacle

et al. 2019

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BackgroundIn soft fruits, the differential expression of many genes during development and ripening is responsible for changing their organoleptic properties. In strawberry fruit, although some genes involved in the metabolic regulation of the ripening process have been functionally characterized, some of the most studied genes correspond to transcription factors. High throughput transcriptomics analyses performed in strawberry red receptacle (Fragaria x ananassa) allowed us to identify a ripening-related gene that codes an atypical HLH (FaPRE1) with high sequence homology with the PACLOBUTRAZOL RESISTANCE (PRE) genes. PRE genes are atypical bHLH proteins characterized by the lack of a DNA-binding domain and whose function has been linked to the regulation of cell elongation processes.ResultsFaPRE1 sequence analysis indicates that this gene belongs to the subfamily of atypical bHLHs that also includes ILI-1 from rice, SlPRE2 from tomato and AtPRE1 from Arabidopsis, which are involved in transcriptional regulatory processes as repressors, through the blockage by heterodimerization of bHLH transcription factors. FaPRE1 presented a transcriptional model characteristic of a ripening-related gene with receptacle-specific expression, being repressed by auxins and activated by abscisic acid (ABA). However, its expression was not affected by gibberellic acid (GA3). On the other hand, the transitory silencing of FaPRE1 transcription by agroinfiltration in receptacle produced the down-regulation of a group of genes related to the ripening process while inducing the transcription of genes involved in receptacle growth and development.ConclusionsIn summary, this work presents for the first time experimental data that support an important novel function for the atypical HLH FaPRE1 during the strawberry fruit ripening. We hypothesize that FaPRE1 modulates antagonistically the transcription of genes related to both receptacle growth and ripening. Thus, FaPRE1 would repress the expression of receptacle growth promoting genes in the ripened receptacle, while it would activate the expression of those genes related to the receptacle ripening process.

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“…Strawberry is considered a plant model system for Rosaceae family due to its small genome size, the short generation time for a perennial species (Folta and Davis, 2006), the easy in vitro regeneration and transformation system (Barceló et al, 1998; Folta et al, 2006; Debnath and Teixeira da Silva, 2007; Qin et al, 2008) and the current availability of different genome sequences of Fragaria species. These include the diploid F. vesca genome (Shulaev et al, 2011) and a virtual strawberry reference genome, named ‘FANhybrid_r1.2,’ obtained by integrating the four homoeologous subgenomes sequences of the octoploid F. X ananassa (Hirakawa et al, 2014).…”

Section: Genetic Analysis and Gene Function Discoverymentioning

confidence: 99%

Transient transformation meets gene function discovery: the strawberry fruit case

Guidarelli

Baraldi

2015

Front. Plant Sci.

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Beside the well known nutritional and health benefits, strawberry (Fragaria X ananassa) crop draws increasing attention as plant model system for the Rosaceae family, due to the short generation time, the rapid in vitro regeneration, and to the availability of the genome sequence of F. X ananassa and F. vesca species. In the last years, the use of high-throughput sequence technologies provided large amounts of molecular information on the genes possibly related to several biological processes of this crop. Nevertheless, the function of most genes or gene products is still poorly understood and needs investigation. Transient transformation technology provides a powerful tool to study gene function in vivo, avoiding difficult drawbacks that typically affect the stable transformation protocols, such as transformation efficiency, transformants selection, and regeneration. In this review we provide an overview of the use of transient expression in the investigation of the function of genes important for strawberry fruit development, defense and nutritional properties. The technical aspects related to an efficient use of this technique are described, and the possible impact and application in strawberry crop improvement are discussed.

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Cited by 69 publications

References 31 publications

Improved regeneration and transformation protocols for three strawberry cultivars

Improved regeneration and transformation protocols for three strawberry cultivars

An atypical HLH transcriptional regulator plays a novel and important role in strawberry ripened receptacle

Transient transformation meets gene function discovery: the strawberry fruit case

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