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Woodruff, Robert W.; Thompson, James N.; Barker, J. S. F.; Huai, Haying

doi:10.1023/a:1003957227608

Cited by 8 publications

(2 citation statements)

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“…A prior study [ 14 ] suggested that transposition into the proximal promoters of Hsp genes can be selectively advantageous because of its impact on Hsp gene expression. Moreover, although deleterious TEs might be inactivated if not purged from populations [ 32 , 33 ], adaptive TEs might be maintained at high frequencies or modified. Surveys of several randomly selected populations ( Table 2 ; Figure 7 ) are consistent with the simultaneous modification and maintenance of TEs.…”

Section: Resultsmentioning

confidence: 99%

Heat-Shock Promoters: Targets for Evolution by P Transposable Elements in Drosophila

2006

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Transposable elements are potent agents of genomic change during evolution, but require access to chromatin for insertion—and not all genes provide equivalent access. To test whether the regulatory features of heat-shock genes render their proximal promoters especially susceptible to the insertion of transposable elements in nature, we conducted an unbiased screen of the proximal promoters of 18 heat-shock genes in 48 natural populations of Drosophila. More than 200 distinctive transposable elements had inserted into these promoters; greater than 96% are P elements. By contrast, few or no P element insertions segregate in natural populations in a “negative control” set of proximal promoters lacking the distinctive regulatory features of heat-shock genes. P element transpositions into these same genes during laboratory mutagenesis recapitulate these findings. The natural P element insertions cluster in specific sites in the promoters, with up to eight populations exhibiting P element insertions at the same position; laboratory insertions are into similar sites. By contrast, a “positive control” set of promoters resembling heat-shock promoters in regulatory features harbors few P element insertions in nature, but many insertions after experimental transposition in the laboratory. We conclude that the distinctive regulatory features that typify heat-shock genes (in Drosophila) are especially prone to mutagenesis via P elements in nature. Thus in nature, P elements create significant and distinctive variation in heat-shock genes, upon which evolutionary processes may act.

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Section: Resultsmentioning

confidence: 99%

Heat-Shock Promoters: Targets for Evolution by P Transposable Elements in Drosophila

2006

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“…Our results are also consistent with previous studies on the impact of P element insertion on the fitness of D. melanogaster. Somatic movement of P has been shown to reduce the life span, fitness, mating ability, and locomotion of D. melanogaster (6,24), whereas other studies have indicated that remobilization of P elements in this species is mainly responsible for the decline in fitness and viability seen in progeny resulting from dysgenic matings (25).…”

Section: Discussionmentioning

confidence: 95%

Assessing fitness costs for transgenicAedes aegyptiexpressing the GFP marker and transposase genes

Irvin

Hoddle

O’Brochta

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

121

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The development of transgenic mosquitoes that are refractory to the transmission of human diseases such as malaria, dengue, and yellow fever has received much interest due to the ability to transform a number of vector mosquito species with transposable elements. Transgenic strains of mosquitoes have been generated with molecular techniques that exhibit a reduced capacity to transmit pathogens. These advancements have led to questions regarding the fitness of transgenic mosquitoes and the ability of transformed mosquitoes to compete and effectively spread beneficial genes through nontransformed field populations, the core requirement of a genetically based control strategy aimed at reducing the spread of mosquito-borne human disease. Here we examine the impact of transgenesis on the fitness of Aedes aegypti, a mosquito that transmits yellow fever. Mosquitoes were altered with two types of transgene, the enhanced GFP gene and two transposase genes from the Hermes and MOS1 transposable elements. We examined the effects of these elements on the survivorship, longevity, fecundity, sex ratio, and sterility of transformed mosquitoes and compared results to the nontransformed laboratory strain. We show that demographic parameters are significantly diminished in transgenic mosquitoes relative to the untransformed laboratory strain. Reduced fitness in transgenic mosquitoes has important implications for the development and utilization of this technology for control programs based on manipulative molecular modification.

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Transposable-Element Vectors and Other Methods to Genetically Modify Drosophila and Other Insects

Hoy¹

2013

Insect Molecular Genetics

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Cited by 8 publications

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Heat-Shock Promoters: Targets for Evolution by P Transposable Elements in Drosophila

Heat-Shock Promoters: Targets for Evolution by P Transposable Elements in Drosophila

Assessing fitness costs for transgenicAedes aegyptiexpressing the GFP marker and transposase genes

Transposable-Element Vectors and Other Methods to Genetically Modify Drosophila and Other Insects

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