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Male antennae of Phyllophaga tristis (Fabricius) (Coleoptera: Scarabaeidae: Melolonthinae) were tested using a coupled gas chromatograph-electroantennogram detector (GC-EAD) system for electrophysiological responses to five sex pheromones identified from other Phyllophaga species including L-valine methyl ester, L-isoleucine methyl ester, L-leucine methyl ester, methyl 2(methylthio)benzoate and methyl 2-amino benzoate. Male antennae responded only to methyl 2(methylthio)benzoate. In a 2003 field test near Greensburg, Kansas, cross-vane traps baited with rubber septa containing 1 mg of methyl 2-(methylthio)benzoate captured 466 male P. tristis. Control traps baited with rubber septa loaded with only hexane captured none. Similarly, in a field test in 2010 in Gainesville, Florida, 265 male P. apicata Reinhard were captured in traps baited with 1 mg of methyl 2-(methylthio)benzoate whereas control traps captured only a single male.

Section: Methodsmentioning

confidence: 99%

“…Neat compounds were diluted in hexane to yield the 20 ng/µl solutions that were used for injection. The GC-EAD instrumentation employed was as described in Robbins et al (2003). …”

Section: Methodsmentioning

confidence: 99%

Methyl 2-(Methylthio)Benzoate: A Sex Attractant for the June Beetles,Phyllophaga tristisandP. apicata

Robbins

Salsbury²,

Woodruff

et al. 2011

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“…The sex pheromone of Phyllophaga (Tostegoptera) lanceolata was identified as L-leucine methyl ester (Nojima et al 2003). Tostegoptera is a small sub-genus of the Phyllophaga consisting of only two species, Phyllophaga (Tostegoptera) lanceolata and Phyllophaga (Tostegoptera) squamipilosa.…”

Section: Discussionmentioning

confidence: 99%

TrappingPhyllophagaspp. (Coleoptera: Scarabaeidae: Melolonthinae) in the United States and Canada using sex attractants

Robbins¹,

Alm²,

Armstrong³

et al. 2006

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The sex pheromone of the scarab beetle, Phyllophaga anxia, is a blend of the methyl esters of two amino acids, L-valine and L-isoleucine. A field trapping study was conducted, deploying different blends of the two compounds at 59 locations in the United States and Canada. More than 57,000 males of 61 Phyllophaga species (Coleoptera: Scarabaeidae: Melolonthinae) were captured and identified. Three major findings included: (1) widespread use of the two compounds [of the 147 Phyllophaga (sensu stricto) species found in the United States and Canada, males of nearly 40% were captured]; (2) in most species intraspecific male response to the pheromone blends was stable between years and over geography; and (3) an unusual pheromone polymorphism was described from P. anxia. Populations at some locations were captured with L-valine methyl ester alone, whereas populations at other locations were captured with L-isoleucine methyl ester alone. At additional locations, the L-valine methyl ester-responding populations and the L-isoleucine methyl ester-responding populations were both present, producing a bimodal capture curve. In southeastern Massachusetts and in Rhode Island, in the United States, P. anxia males were captured with blends of L-valine methyl ester and L-isoleucine methyl ester.

“…The methodology used in the present study was similar to prior studies (Zhang et al 1997; Leal et al 2003; Nojima et al 2003; Robbins et al 2003, 2006, 2008, 2009; Alm et al 2004) that captured Phyllophaga adults using sex attractants. Cross-vane traps (see Robbins et al 2006) were hung on a series of metal stakes such that the trap bottom was 1 m above the ground.…”

Section: Methodsmentioning

confidence: 99%

“…After emerging from the soil, females release sex pheromones by opening the pygidium and extruding a gland from which the pheromone volatilizes (for photos, see Leal et al 1993; Nojima et al 2003; Zarbin et al 2007). Sex pheromones identified from the Phyllophaga include methyl 2-(methylthio) benzoate from P. crinita (Robbins et al 2003); methyl esters of three amino acids, including L-valine, from P. anxia and P. (Phytalus) georgiana (Zhang et al 1997; Robbins et al 2009); L-isoleucine from P. anxia and P. elenans (Zhang et al 1997; Leal et al 2003); L-leucine from P. lanceolata, (Nojima et al 2003); and phenol and p-cresol from P. cuyabana (Zarbin et al 2007). Furthermore, an extensive trapping study by Robbins et al (2006) demonstrated the widespread use of the methyl esters of L-valine and L-isoleucine methyl ester in the mate-finding systems of >50 species of Phyllophaga in the United States and Canada.…”

Section: Introductionmentioning

confidence: 99%

First Record of the Scarab Beetle,Phyllophaga lissopygefrom South America, with Descriptions of Adult Seasonal Activity and Male Response to Sex Attractants

Morales-Rodríguez

Peck

Robbins

2011

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Phyllophaga lissopyge (Bates) (Coleoptera: Scarabaeidae: Melolonthinae) is reported for the first time from South America. Male sex pheromone response is described for P. lissopyge and two other co-occurring Phyllophaga species. Adults of P. lissopyge and P. menetriesi (Blanchard) flew to traps baited with methyl 2-(methylthio) benzoate whereas adults of P. obsoleta (Blanchard) flew irregularly to four different pheromone compounds. Adult seasonal activity is described from males captures in Rionegro, Antioquia, Colombia.