[37] Simultaneous quantitation and separation of carotenoids and retinol in human milk by high-performance liquid chromatography

Giuliano, Anna R.; Neilson, Eleanor M.; Kelly, B.E.; Canfield, Louise M.

doi:10.1016/0076-6879(92)13141-j

Cited by 70 publications

(59 citation statements)

References 7 publications

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“…The breast milk retinol was extracted according to Giuliano et al (1992), with adaptations. The method consisted of an alkaline hydrolysis and protein denaturation, using the potassium hydroxide (50%) and ethyl alcohol 95% as reactants, respectively.…”

Section: Methodsmentioning

confidence: 99%

Gestational diabetes: a risk of puerperal hypovitaminosis A?

Resende

Lira

Grilo

et al. 2015

An. Acad. Bras. Ciênc.

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The influence of gestational diabetes on vitamin A deficiency in lactating women and, consequently, in their newborn has been verified through a cross-sectional case-control study conducted with volunteer puerperal women. The control group consisted of healthy women and the test group was composed of women with gestational diabetes. One hundred and seven women were recruited, corresponding to 71 controls and 36 cases. Personal, gestational and newborn data were collected directly from medical records during hospitalization. The retinol was determined in maternal colostrum and serum by High Performance Liquid Chromatography. Postpartum women with gestational diabetes were older, had more children and a higher prevalence of cesarean delivery. No difference was found in retinol concentration in maternal milk and serum between the groups. However, it was observed that 16.7% had vitamin A deficiency in the group of patients with diabetes and only 4.1% had such deficiency in the control group. Although no difference was found in colostrum and serum retinol concentration between women with and without gestational diabetes, the individual analysis shows that those with diabetes are at higher risk of being vitamin A deficient.

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Section: Methodsmentioning

confidence: 99%

Gestational diabetes: a risk of puerperal hypovitaminosis A?

Resende

Lira

Grilo

et al. 2015

An. Acad. Bras. Ciênc.

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“…12 The remaining quantity of milk was then used for retinol extraction, using the method described by Giuliano et al 13 modified as follows.…”

Section: Introductionmentioning

confidence: 99%

Avaliação dos níveis de retinol no colostro humano coletado no intervalo de 24 horas

Ribeiro

Araújo²,

Pereira³

et al. 2007

J. Pediatr. (Rio J.)

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Objective: To evaluate retinol concentration in colostrum samples collected with a 24 hour interval.Methods: Colostrum was collected from 24 recently-delivered mothers at two points in time, 0 hours (T0) and 24 hours later (T24), and a pooled sample of colostrum from T0 and T24 was also analyzed. Fat content was determined by creamatocrit, and retinol assayed by high performance liquid chromatography.Results: When expressed in terms of volume of milk (µg/dL), retinol levels varied across T0, T24 and the pooled sample: 94.9±58.9, 129±78.6 and 111.9±60.4 µg/dL, respectively. However, when expressed with relation to fat content (µg/g), no significant difference was observed.

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“…De cada recipiente foram retiradas dez alíquotas, cada qual contendo 1 mL de leite. A extração foi realizada segundo Giuliano et al 17 , modificada, como descrito a seguir. Após quantificar o volume de leite coletado foram acrescentados nas amostras hidróxido de potássio 50% v/v (Vetec) e álcool etílico 95% (Vetec), para a etapa de hidrólise alcalina.…”

Section: Parte Experimentalunclassified