35. The role of aquaporin-3 in the movement of water and cryoprotectants across the plasma membrane in mouse morulae

Edashige, Keisuke; Ota, Satoshi; Tanaka, Mitsunobu; Valdez, Delgado M.; Seki, Shinsuke; Hara, Takao; Jin, Bo; Kasai, Magosaburo

doi:10.1016/j.cryobiol.2006.10.036

Cited by 16 publications

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“…In previous studies, we showed that the pattern of movement of water and cryoprotectants did not change markedly from mature oocytes to embryos at early cleavage stages in the mouse, whereas a marked change was observed at around the morula stage [12][13][14]; the permeability of embryos to water, glycerol, and ethylene glycol markedly increased and that to acetamide and dimethyl sulfoxide (DMSO) also increased slightly but significantly, whereas permeability to propylene glycol did not change significantly. This suggests that cryoprotectants permeate mouse embryos through different pathways, which depend not only on the developmental stage, but also on the cryoprotectant itself.…”

Section: Introductionmentioning

confidence: 82%

“…One is simple diffusion through the lipid bilayer, and the other is facilitated diffusion via channel processes. We previously showed that water and cryoprotectants moved through the oocytes and embryos of mice and cows at early cleavage stages predominantly by simple diffusion, and that water and some cryoprotectants moved through embryos at later stages (morulae and possibly blastocysts) predominantly by facilitated diffusion via channel processes [13][14][15]. We also demonstrated that aquaporin 3 was a major contributor to facilitated diffusion by water, glycerol, and ethylene glycol [14,15].…”

Section: Introductionmentioning

confidence: 87%

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Rapid Movement of Water and Cryoprotectants in Pig Expanded Blastocysts via Channel Processes: Its Relevance to Their Higher Tolerance to Cryopreservation1

Jin

Higashiyama

Nakata

et al. 2013

Biology of Reproduction

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Pig oocytes and embryos are highly sensitive to cryopreservation; however, tolerance to cryopreservation increases in embryos at the expanded blastocyst stage. This increased tolerance may be attributed to a decrease in cytoplasmic lipid droplets at this stage. We previously showed that an increase in the permeability of the plasma membrane in mouse oocytes to water and cryoprotectants, caused by the artificial expression of aquaporin 3, an aquaglyceroporin, enhanced tolerance to cryopreservation. In the present study, we investigated whether membrane permeability was also involved in the tolerance of pig embryos to cryopreservation. The permeability of oocytes and morulae to water and glycerol was low, whereas that of expanded blastocysts was high. Activation energy for permeability to water, glycerol, ethylene glycol, and dimethyl sulfoxide was markedly lower for expanded blastocysts than for oocytes. This suggests that water and these cryoprotectants move through expanded blastocysts predominantly by facilitated diffusion and through oocytes predominantly by simple diffusion. Aquaporin 3 mRNA was expressed in expanded blastocysts abundantly, but less so in oocytes. On the other hand, the permeability of expanded blastocysts to propylene glycol was as low as that of oocytes, and activation energy for its permeability was similar to that of oocytes, which suggests that propylene glycol moves through oocytes and embryos predominantly by simple diffusion. These results suggest that the higher tolerance of pig expanded blastocysts to cryopreservation is also related to high membrane permeability due to the expression of water/cryoprotectant channels, in addition to the decrease in cytoplasmic lipid droplets.

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Section: Introductionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 87%

Rapid Movement of Water and Cryoprotectants in Pig Expanded Blastocysts via Channel Processes: Its Relevance to Their Higher Tolerance to Cryopreservation1

Jin

Higashiyama

Nakata

et al. 2013

Biology of Reproduction

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“…We also showed in the mouse that permeability to water and cryoprotectants was low in oocytes and early embryos, whereas permeability to water, glycerol and ethylene glycol was high in morulae [4], and that aquaporin 3 (AQP3), an aquaglyceroporin, plays an important role in the high permeability [5,6]. Therefore, the high tolerance of morulae to cryopreservation would be attributable to their high permeability via water/cryoprotectant channels.…”

mentioning

confidence: 93%

“…[ 5,6]. Since the glycerol-permeability was quite low, mouse oocytes could not survive after vitrification with a glycerol-based solution.…”

Section: Development Of Vitrified Mouse Oocytes Expressing Aqp3mentioning

confidence: 99%

Developmental Ability of Vitrified Mouse Oocytes Expressing Water Channels

Yamaji

Seki

Matsukawa

et al. 2011

Journal of Reproduction and Development

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Abstract. Previously, we showed that the exogenous expression of aquaporin 3 (AQP3), an aquaglyceroporin, improved the tolerance of mouse oocytes to vitrification with a glycerol-based solution. In the present study, we examined conditions suitable for the expression of AQP3 and the ability of vitrified oocytes to develop in vitro and in vivo after fertilization. After only partial remove of cumulus cells, immature mouse oocytes (germinal vesicle stage) were injected with 5, 10 or 20 pg of AQP3 cRNA and cultured for 12 h for maturation. When matured oocytes were vitrified with a glycerol-based solution, 57-61% survived regardless of the amount of cRNA injected (5-20 pg). By contrast, no oocytes injected with water (control) survived. When the zona pellucida was removed from the vitrified oocytes and the oocytes were then fertilized in vitro and cultured, the proportions that were fertilized and developed into blastocysts were higher when the amount of cRNA injected was 5 pg than 10-20 pg. When 16 blastocysts were transferred to a pseudopregnant mouse, 5 developed to term, demonstrating that oocytes vitrified after injection of AQP3 cRNA retained the ability to develop to term. The water-permeability of cRNA-injected oocytes was higher than that of control oocytes from the maturing stage to the 1-cell zygote stage, whereas glycerol-permeability was higher only at metaphase II. This indicates that AQP3 was expressed for a relatively short period of time. These results suggest that the transient expression of water/cryoprotectant channels is effective for cryopreserving cells that have low membrane-permeability, such as mammalian oocytes. Key words: Aquaporin, Development, Membrane-permeability, Oocyte, Vitrification (J. Reprod. Dev. 57: [403][404][405][406][407][408] 2011) he permeability of the plasma membrane to water and cryoprotectants is one of the most important factors for successful cryopreservation of oocytes/embryos because it influences the formation of intracellular ice, the toxicity of cryoprotectants and osmotic swelling [1]. For cryopreservation of oocytes/embryos, vitrification has advantages over slow freezing in terms of the simplicity of the method and viability of the cells. As the solution used for vitrification contains a high concentration of cryoprotectants, it is much more toxic than the solution for slow-freezing. Therefore, the permeability of the plasma membrane would affect the survival of cryopreserved cells more in vitrification than in slow freezing. If the permeability is low, insufficient exposure to the cryoprotectants causes formation of intracellular ice. However, excessive exposure results in toxic effects of the cryoprotectants. Therefore, high permeability to water and cryoprotectants would be preferable for vitrification.We showed that a simple one-step method is effective for vitrification of mouse morulae [2] but that a two-step method with a pretreatment with a lower concentration of the cryoprotectant is favorable for vitrifying mouse embryos at early cleavage stages...

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“…This model has been used extensively in cryobiology (see, for example, [13,15,16,[21][22][23][24][25][26][27][28]) and in a wide variety of other applied and theoretical settings [5,17,[29][30][31][32][33][34], including Reuss et al [35] who model two permeating 1 Concentration and molality are essentially equivalent in near ideal-dilute conditions. 2 solutes and comment explicitly about the absence of an exact solution.…”

Section: Introductionmentioning

confidence: 99%

Global stability and exact solution of an arbitrary-solute nonlinear cellular mass transport system

Benson¹

2014

Mathematical Biosciences

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35. The role of aquaporin-3 in the movement of water and cryoprotectants across the plasma membrane in mouse morulae

Cited by 16 publications

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Rapid Movement of Water and Cryoprotectants in Pig Expanded Blastocysts via Channel Processes: Its Relevance to Their Higher Tolerance to Cryopreservation1

Rapid Movement of Water and Cryoprotectants in Pig Expanded Blastocysts via Channel Processes: Its Relevance to Their Higher Tolerance to Cryopreservation1

Developmental Ability of Vitrified Mouse Oocytes Expressing Water Channels

Global stability and exact solution of an arbitrary-solute nonlinear cellular mass transport system

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