Expression of potential reference genes in response to macronutrient stress in rice and soybean

Vengavasi, Krishnapriya; Kumar, Manu; Yadav, Shiv Kumar; Pandey, Renu

doi:10.1016/j.gene.2021.145742

Cited by 8 publications

(1 citation statement)

References 65 publications

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“…A standard curve for each set of primers was generated using five serially diluted cDNA samples as templates. E values were estimated as described previously [25]. E values ranged from 0.91 to 1.05 and R 2 ranged from 0.98 to 0.99, respectively (Figure S1).…”

Section: Qrt-pcr Verificationmentioning

confidence: 99%

Identification of Candidate Genes for Drought Resistance during Soybean Seed Development

et al. 2023

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Drought is the main abiotic stress limiting soybean production worldwide and seriously limits the reproductive growth of soybean. To elucidate the molecular mechanisms of drought stress response in soybean seed developmental stages, transcriptome analysis was performed on five seed developmental stages under drought stress and control conditions. Thousands of genes were found to change in expression level under drought stress, GO and KEGG enrichment analysis showed that differentially expressed genes (DEGs) were involved in drought resistance at every stage or tissue, but there were very few DEGs at the primary stage of seed development, which indicated that the seeds at the primary developmental stage were influenced little by drought stress. There was no shared DEG among all five stages or tissues, this indicated that they have different mechanisms to resist drought stress. Furthermore, we investigated DEGs associated with drought response at different stages to identify candidate regulators of drought stress response during soybean seed development. These genes are associated with metabolic and regulatory networks, among others. Taken together, the results will expand our understanding of the adaptation of soybean to drought stress and provide new insights into the molecular mechanisms of soybean drought tolerance development.

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Section: Qrt-pcr Verificationmentioning

confidence: 99%