A Mouse Model of Candidiasis

Fajardo, Pilar; Cuenda, Ana; Sanz‐Ezquerro, Juan José

doi:10.1007/978-1-0716-1488-4_7

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…Compared to polymicrobial sepsis, monomicrobial sepsis is more common in humans. There are many protocols available using different bacterial species to induce sepsis (He et al., 2021; LeBlanc & Lefort, 2021; Spencer et al., 2021), while fungal models of sepsis are also prevalent (Carreras et al., 2019; Fajardo et al., 2021). In each of the models, evaluating systemic inflammation, lymphopenia, temperature changes, and weight loss will be guides to determine a sepsis‐causing pathogen dose used.…”

Section: Commentarymentioning

confidence: 99%

Mouse Models of Sepsis

Kannan,

Kim,

Heidarian

et al. 2024

Current Protocols

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Human sepsis is a complex disease that manifests with a diverse range of phenotypes and inherent variability among individuals, making it hard to develop a comprehensive animal model. Despite this difficulty, numerous models have been developed that capture many key aspects of human sepsis. The robustness of these models is vital for conducting pre‐clinical studies to test and develop potential therapeutics. In this article, we describe four different models of murine sepsis that can be used to address different scientific questions relevant to the pathology and immune response during and after a septic event. Basic Protocol 1 details a non‐synchronous cecal ligation and puncture (CLP) model of sepsis, where mice are subjected to polymicrobial exposure through surgery at different time points within 2 weeks. This variation in sepsis onset establishes each mouse at a different state of inflammation and cytokine levels that mimics the variability observed in humans when they present in the clinic. This model is ideal for studying the long‐term impact of sepsis on the host. Basic Protocol 2 is also a type of polymicrobial sepsis, where injection of a specific amount of cecal slurry from a donor mouse into the peritoneum of recipient mice establishes immediate inflammation and sepsis without any need for surgery. Basic Protocol 3 describes infecting mice with a defined gram‐positive or ‐negative bacterial strain to model a subset of sepsis observed in humans infected with a single pathogen. Basic Protocol 4 describes administering LPS to induce sterile endotoxemia. This form of sepsis is observed in humans exposed to bacterial toxins from the environment. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Non‐synchronous cecal ligation and punctureBasic Protocol 2: Cecal slurry model of murine sepsisBasic Protocol 3: Monomicrobial model of murine sepsisBasic Protocol 4: LPS model of murine sepsis

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Section: Commentarymentioning

confidence: 99%

Mouse Models of Sepsis

Kannan,

Kim,

Heidarian

et al. 2024

Current Protocols

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“…In intensive care units, candidemia is reported as the fourth most common bloodstream infection [39]. When the fungus is introduced in systemic circulation, mice have been shown to develop kidney failure and septic shock reflective of human sepsis [40]. Others have shown that mice infected with live C. albicans develop clotting disorders such as decreased clotting times and thrombocytopenia [41].…”

Section: Bacterial and Fungal Infection Modelsmentioning

confidence: 99%

Advances in Rodent Experimental Models of Sepsis

Cai,

Rodgers,

Schoenmann

et al. 2023

IJMS

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In the development of therapeutic strategies for human diseases, preclinical experimental models have a key role. However, the preclinical immunomodulatory therapies developed using rodent sepsis were not successful in human clinical trials. Sepsis is characterized by a dysregulated inflammation and redox imbalance triggered by infection. Human sepsis is simulated in experimental models using methods that trigger inflammation or infection in the host animals, most often mice or rats. It remains unknown whether the characteristics of the host species, the methods used to induce sepsis, or the molecular processes focused upon need to be revisited in the development of treatment methods that will succeed in human clinical trials. Our goal in this review is to provide a survey of existing experimental models of sepsis, including the use of humanized mice and dirty mice, and to show how these models reflect the clinical course of sepsis. We will discuss the strengths and limitations of these models and present recent advances in this subject area. We maintain that rodent models continue to have an irreplaceable role in studies toward discovering treatment methods for human sepsis.

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A Mouse Model of Candidiasis

Cited by 2 publications

References 15 publications

Mouse Models of Sepsis

Mouse Models of Sepsis

Advances in Rodent Experimental Models of Sepsis

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