Analysis of Extracellular Cell Wall Lipids: Wax, Cutin, and Suberin in Leaves, Roots, Fruits, and Seeds

Baales, Johanna; Zeisler‐Diehl, Viktoria; Schreiber, Lukas

doi:10.1007/978-1-0716-1362-7_15

Cited by 11 publications

(13 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Four to eight replicates were analysed per acclimatisation step. Cuticular waxes were extracted by completely submerging each leaf in 5 ml of chloroform for 10 s. Extraction kinetics conducted for leaf wax have shown that 10 s of extraction time are long enough for a complete wax extraction (Baales et al, 2021). After extraction, the leaves were scanned for leaf surface area determination.…”

Section: Analysis and Quantification Of Cuticular Wax Compositionmentioning

confidence: 99%

“…Wax compositions of leaves grown in the environmental conditions experiment were analysed by tightly placing broad rim vials (1.28 cm 2 opening) filled with chloroform on the adaxial leaf sides (Baales et al, 2021). Wax was extracted repeatedly turning the vials closed with the leaf upside down and shaking for 10 s. For each of the four biological replicates investigated, four extracts per leaf were pooled.…”

Section: Analysis and Quantification Of Cuticular Wax Compositionmentioning

confidence: 99%

See 1 more Smart Citation

Increased cuticular wax deposition does not change residual foliar transpiration

Grünhofer

Herzig

Sent

et al. 2022

Plant Cell & Environment

Self Cite

View full text Add to dashboard Cite

The effect of contrasting environmental growth conditions (in vitro tissue culture, ex vitro acclimatisation, climate chamber, greenhouse and outdoor) on leaf development, cuticular wax composition, and foliar transpiration of detached leaves of the Populus × canescens clone 84 K were investigated. Our results show that total amounts of cuticular wax increased more than 10-fold when cultivated in different growth conditions, whereas qualitative wax composition did not change. With exception of plants directly taken from tissue culture showing rapid dehydration, rates of water loss (residual foliar transpiration) of intact but detached leaves were constant and independent from growth conditions and thus independent from increasing wax amounts. Since cuticular transpiration measured with isolated astomatous P. × canescens cuticles was identical to residual foliar transpiration rates of detached leaves, our results confirm that cuticular transpiration of P. × canescens leaves can be predicted with high accuracy from residual transpiration of detached leaves after stomatal closure. Our results convincingly show that more than 10-fold increased wax amounts in P. × canescens cuticles do not lead to decreased rates of residual (cuticular) transpiration.

show abstract

Section: Analysis and Quantification Of Cuticular Wax Compositionmentioning

confidence: 99%

Section: Analysis and Quantification Of Cuticular Wax Compositionmentioning

confidence: 99%

Increased cuticular wax deposition does not change residual foliar transpiration

Grünhofer

Herzig

Sent

et al. 2022

Plant Cell & Environment

Self Cite

View full text Add to dashboard Cite

show abstract

“…Wax analysis of the suberized tissues was performed as described in Baales et al ( 2021 ). Wax was extracted by immersing isolated suberized tissues in chloroform (5 ml) overnight in glass vials kept on a rolling bench.…”

Section: Methodsmentioning

confidence: 99%

“…The total solvent volume of wax extracts was reduced under a gentle stream of nitrogen gas to a final volume of 200 µl. For suberin analysis, wax-extracted samples were depolymerized using boron trifluoride/methanol (BF 3 /MeOH, Fluka) as described by Baales et al ( 2021 ). Before extraction of the released monomers with chloroform, transesterified samples were spiked with 20 µg of dotriacontane (100 µl of a solution of 10 mg dotriacontane in 50 ml chloroform; Fluka) as internal standard.…”

Section: Methodsmentioning

confidence: 99%

Comparing anatomy, chemical composition, and water permeability of suberized organs in five plant species: wax makes the difference

Suresh

Zeisler‐Diehl

Wojciechowski

et al. 2022

Planta

Self Cite

View full text Add to dashboard Cite

Main conclusion The efficiency of suberized plant/environment interfaces as transpiration barriers is not established by the suberin polymer but by the wax molecules sorbed to the suberin polymer. Abstract Suberized cell walls formed as barriers at the plant/soil or plant/atmosphere interface in various plant organs (soil-grown roots, aerial roots, tubers, and bark) were enzymatically isolated from five different plant species (Clivia miniata, Monstera deliciosa, Solanum tuberosum, Manihot esculenta, and Malus domestica). Anatomy, chemical composition and efficiency as transpiration barriers (water loss in m s−1) of the different suberized cell wall samples were quantified. Results clearly indicated that there was no correlation between barrier properties of the suberized interfaces and the number of suberized cell layers, the amount of soluble wax and the amounts of suberin. Suberized interfaces of C. miniata roots, M. esculenta roots, and M. domestica bark periderms formed poor or hardly any transpiration barrier. Permeances varying between 1.1 and 5.1 × 10−8 m s−1 were very close to the permeance of water (7.4 × 10−8 m s−1) evaporating from a water/atmosphere interface. Suberized interfaces of aerial roots of M. deliciosa and tubers of S. tuberosum formed reasonable transpiration barriers with permeances varying between 7.4 × 10−10 and 4.2 × 10−9 m s−1, which were similar to the upper range of permeances measured with isolated cuticles (about 10−9 m s−1). Upon wax extraction, permeances of M. deliciosa and S. tuberosum increased nearly tenfold, which proves the importance of wax establishing a transpiration barrier. Finally, highly opposite results obtained with M. esculenta and S. tuberosum periderms are discussed in relation to their agronomical importance for postharvest losses and tuber storage.

show abstract

“…The dried, delipidated leaf samples were transesterified with BF 3 -methanol to release cutin monomers (Kolattukudy & Agrawal, 1974). Gas chromatographic analysis and mass spectrometric identification were performed as described earlier (Baales et al, 2021). At least three independent biological replicates were used for each experiment.…”

Section: Chemical Analysis Of Wax and Cutinmentioning

confidence: 99%

Cuticular transpiration is not affected by enhanced wax and cutin amounts in response to osmotic stress in barley

Shellakkutti

Thangamani

Suresh

et al. 2022

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

The plant cuticle, which covers all aerial parts of plants in their primary developmental stage, is the major barrier against water loss from leaves. Accumulation of cutin and waxes has often been linked to drought tolerance. Here we investigated whether cutin and waxes play a role in the drought adaption of barley mimicked by osmotic stress acting on roots. We compared the cuticle properties of cultivated barley (Hordeum vulgare spp. vulgare) with wild barley (Hordeum vulgare spp. spontaneum), and tested whether wax and cutin composition or amount and cuticular transpiration could be future breeding targets for more drought‐tolerant barley lines. In response to osmotic stress, accumulation of wax crystals was observed. This coincides with an increased wax and cutin gene expression and a total increase of wax and cutin amounts in leaves, which seems to be a general response triggered through root shoot signalling. Stomatal conductance decreased fast and significantly, whereas cuticular conductance remained unaffected in both wild and cultivated barley. The often‐made conclusion that higher amounts of wax and cutin necessarily reduce cuticular transpiration and thus enhance drought tolerance is not always straightforward. To prevent water loss, stomatal regulation under water stress is much more important than regulation or adaptation of cuticular transpiration in response to drought.

show abstract

Analysis of Extracellular Cell Wall Lipids: Wax, Cutin, and Suberin in Leaves, Roots, Fruits, and Seeds

Cited by 11 publications

References 8 publications

Increased cuticular wax deposition does not change residual foliar transpiration

Increased cuticular wax deposition does not change residual foliar transpiration

Comparing anatomy, chemical composition, and water permeability of suberized organs in five plant species: wax makes the difference

Cuticular transpiration is not affected by enhanced wax and cutin amounts in response to osmotic stress in barley

Contact Info

Product

Resources

About