In vivo effectiveness of hybrid membranes with osteogenic growth peptide for bone regeneration

Oshiro, João Augusto; Barros, Rafaela Magalhães; Silva, Camila Garcia; Souza, Caroline Cordeiro; Scardueli, Cássio Rocha; Saches, Paulo Ricardo Silva; Mendes, Larissa Souza; Cilli, Eduardo Maffud; Marcantonio, Camila Chierici; Chiavacci, Leila Aparecida

doi:10.1002/term.3226

Cited by 5 publications

(5 citation statements)

References 36 publications

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“…Thus, assuming a cylindrical shape, the values of the n parameter ( Table S2 ) were above 0.88 for all curves, corresponding to the transport mechanism of case II, where the difference concerning the anomalous transport is caused only by the order of velocity of the release processes (diffusion and swelling). Therefore, the DMA diffusion velocity is higher with the relaxation of the material chains, which contributes to the prolonged release observed in this study [ 28 , 29 ]. Macroscopic swelling and drug release test results allowed us to analyze and rationally select the membrane u-PPO400/2000 (70:30) for the following experiments.…”

Section: Resultsmentioning

confidence: 99%

Dexamethasone-Loaded Ureasil Hydrophobic Membrane for Bone Guided Regeneration

et al. 2022

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Physical barrier membranes have been used to release active substances to treat critical bone defects; however, hydrophilic membranes do not present a prolonged release capacity. In this sense, hydrophobic membranes have been tested. Thus, this study aimed to develop hydrophobic membranes based on mixtures of ureasil–polyether-type materials containing incorporated dexamethasone (DMA) for the application in guided bone regeneration. The physicochemical characterization and biological assays were carried out using small-angle X-ray scattering (SAXS), an in vitro DMA release study, atomic force microscopy (AFM), a hemolysis test, and in vivo bone formation. The swelling degree, SAXS, and release results revealed that the u-PPO400/2000 membrane in the proportion of 70:30 showed swelling (4.69% ± 0.22) similar to the proportions 90:10 and 80:20, and lower than the proportion 60:40 (6.38% ± 0.49); however, an equal release percentage after 134 h was observed between the proportions 70:30 and 60:40. All u-PPO materials presented hemocompatibility (hemolysis ≤2.8%). AFM results showed that the treatments with or without DMA did not present significant differences, revealing a flat/smooth surface, with no pores and/or crystalline precipitates. Finally, in vivo results revealed that for both the commercial hydrophilic membrane and u-PPO400/2000 (70:30) after 60 days, the bone formation volume was 21%. In conclusion, hybrid membranes present unique characteristics for treating critical bone defects, considering the delayed and prolonged release results associated with the physical barrier capacity.

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Section: Resultsmentioning

confidence: 99%

Dexamethasone-Loaded Ureasil Hydrophobic Membrane for Bone Guided Regeneration

et al. 2022

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“…Ureasil–polyether hybrid materials stand out for their mechanical strength, easy manipulation, biocompatibility, and the ability to incorporate high quantities of hydrophilic or lipophilic drugs and control their release profile. This makes them an excellent choice for drug delivery applications where targeted and controlled release are desired [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…The high flexibility of these materials makes it possible to develop different pharmaceutical forms from these systems, such as vaginal ovules [ 18 ]. These formulations usually contain one or more active compounds that is dispersed or dissolved in a base that melts or dissolves at body temperature and can be inserted into the vagina without irritation, as well as ensuring uniform doses and not requiring a high volume of fluid for dissolution [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

The Efficacy of Hybrid Vaginal Ovules for Co-Delivery of Curcumin and Miconazole against Candida albicans

Bezerra,

y Araújo,

Alves-Júnior

et al. 2024

Pharmaceutics

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Curcumin (CUR) is a natural compound that can be combined with miconazole (MCZ) to improve vulvovaginal candidiasis (VVC) caused by Candida albicans treatment’s efficacy. This study aimed to develop ureasil–polyether (U-PEO) vaginal ovules loaded with CUR and MCZ for the treatment of VVC. Physicochemical characterization was performed by thermogravimetry (TGA), differential thermal analysis (DTA), Fourier transform infrared spectroscopy (FTIR), and in vitro release. Antifungal assays were used to determine minimum inhibitory concentrations (MICs) and synergism between CUR and MCZ, and the activity of U-PEO ovules were performed by microdilution and agar diffusion. TGA results showed high thermal stability of the hybrid ovules. In DTA, the amorphous character of U-PEO and a possible interaction between CUR and MCZ were observed. FTIR showed no chemical incompatibility between the drugs. In vitro release resulted in 80% of CUR and 95% of MCZ released within 144 h. The MICs of CUR and MCZ were 256 and 2.5 µg/mL, respectively. After combining the drugs, the MIC of MCZ decreased four-fold to 0.625 µg/mL, while that of CUR decreased eight-fold to 32 µg/mL. Synergism was confirmed by the fractional inhibitory concentration index (FICI) equal to 0.375. U-PEO alone showed no antifungal activity. U-PEO/MCZ and U-PEO/CUR/MCZ ovules showed the greatest zones of inhibition (≥18 mm). The results highlight the potential of the ovules to be administered at a lower frequency and at reduced doses compared to available formulations.

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“…Thus, organic–inorganic hybrid polymeric materials have expanded in their development since the 1980s [ 27 , 28 , 29 ], and it is a unique material, formed by the mixture on a nanometric scale of organic (or biological) and inorganic components, synergistically combining the physicochemical characteristics of their components. This allows for the achievement of unique properties, making this class of materials excellent for the development of new multifunctional systems with broad applications, such as membrane separation processes, drug delivery systems, implants, and others [ 27 , 30 , 31 , 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

“…For example, in vitro results revealed that the mixture, in the proportions of 80% ureasil-POE and 20% ureasil-POP, released 21% of the osteogenic growth peptide after 48 h and exhibited zero order release mechanisms. This is ideal for drug release since it allows for sustained active release in the same amount per unit time [ 31 ]. The flexibility of membranes can also be improved with the use of the polymeric blends to become more easily manageable during the implant process [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Hybrid Membranes of the Ureasil-Polyether Containing Glucose for Future Application in Bone Regeneration

et al. 2023

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The application of mesenchymal stem cells (MSC) in bone tissue regeneration can have unpredictable results due to the low survival of cells in the process since the lack of oxygen and nutrients promotes metabolic stress. Therefore, in this work, polymeric membranes formed by organic–inorganic hybrid materials called ureasil-polyether for modified glucose release were developed in order to overcome the problems posed by a of lack of this nutrient. Thus, membranes formed by polymeric blend of polypropylene oxide (PPO4000) and polyethylene oxide (PEO500) with 6% glucose incorporation were developed. Physical–chemical characterization techniques were performed, as well as tests that evaluated thermal properties, bioactivity, swelling, and release in SBF solution. The results of the swelling test showed an increase in membrane mass correlated with an increase in the concentration of ureasil-PEO500 in the polymeric blends. The membranes showed adequate resistance when subjected to the application of a high compression force (15 N). X-ray diffraction (XRD) evidenced peaks corresponding to orthorhombic crystalline organization, but the absence of glucose-related peaks showed characteristics of the amorphous regions of hybrid materials, likely due to solubilization. Thermogravimetry (TG) and differential scanning calorimetry (DSC) analyses showed that the thermal events attributed to glucose and hybrid materials were similar to that seen in the literature, however when glucose was incorporated into the PEO500, an increase in rigidity occurs. In PPO400, and in the blends of both materials, there was a slight decrease in Tg values. The smaller contact angle for the ureasil-PEO500 membrane revealed the more hydrophilic character of the material compared to other membranes. The membranes showed bioactivity and hemocompatibility in vitro. The in vitro release test revealed that it is possible to control the release rate of glucose and the kinetic analysis revealed a release mechanism characteristic of anomalous transport kinetics. Thus, we can conclude that ureasil-polyether membranes have great potential to be used as a glucose release system, and their future application has the potential to optimize the bone regeneration process.

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In vivo effectiveness of hybrid membranes with osteogenic growth peptide for bone regeneration

Cited by 5 publications

References 36 publications

Dexamethasone-Loaded Ureasil Hydrophobic Membrane for Bone Guided Regeneration

Dexamethasone-Loaded Ureasil Hydrophobic Membrane for Bone Guided Regeneration

The Efficacy of Hybrid Vaginal Ovules for Co-Delivery of Curcumin and Miconazole against Candida albicans

Hybrid Membranes of the Ureasil-Polyether Containing Glucose for Future Application in Bone Regeneration

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