Plastid Transformation of Sporelings from the Liverwort Marchantia polymorpha L.

Chiyoda, Shota; Yamato, Katsuyuki T.; Kohchi, Takayuki

doi:10.1007/978-1-0716-1472-3_20

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“…Plastid transformation in M. polymorpha has been established with a combination of rapidly proliferating sporelings and particle bombardment ( Chiyoda et al, 2007 ; Chiyoda et al, 2021 ). In general, multiple rounds of selective steps are required to obtain homoplasmic plastid transformants in other plant species, but only a primary selection is sufficient for M. polymorpha , providing opportunities for functional analyses of plastid-encoded genes ( Ueda et al, 2012 ; Ueda et al, 2014 ) including the development of a plastid reporter system ( Boehm et al, 2016 ).…”

Section: Geneticsmentioning

confidence: 99%

The renaissance and enlightenment ofMarchantiaas a model system

et al. 2022

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The liverwort Marchantia polymorpha has been utilized as a model for biological studies since the 18th century. In the past few decades there has been a Renaissance in its utilization in genomic and genetic approaches to investigating physiological, developmental, and evolutionary aspects of land plant biology. The reasons for its adoption are similar to those of other genetic models, e.g. simple cultivation, ready access via its worldwide distribution, ease of crossing, facile genetics, and more recently, efficient transformation, genome editing, and genomic resources. The haploid gametophyte dominant life cycle of M. polymorpha is conducive to forward genetic approaches. The lack of ancient whole-genome duplications within liverworts facilitates reverse genetic approaches, and possibly related to this genomic stability, liverworts possess sex chromosomes that evolved in the ancestral liverwort. As a representative of one of the three bryophyte lineages, its phylogenetic position allows comparative approaches to provide insights into ancestral land plants. Given the karyotype and genome stability within liverworts, the resources developed for M. polymorpha have facilitated the development of related species as models for biological processes lacking in M. polymorpha.

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