Transcriptome profiling analysis of the response to walnut polyphenol extract in <i>Helicobacter pylori</i>-infected cells

Abstract

Dietary intervention to prevent Helicobacter pylori (H. pylori) associated gastric diseases seems to be ideal with no risk of bacterial resistance, safe long term intervention, and correcting pathogenic mechanisms including rejuvenation of precancerous atrophic gastritis and anti mutagenesis. A transcriptome as set of all RNAs transcribed by certain tissues or cells demonstrates gene functions and reveals the molecular mechanism of specific biological processes against diseases. Here, we have performed RNAseq and bioinformatic analysis to explain proof of concept that walnut intake can rescue from H. pylori infection and explore unidentified mode of actions of walnut polyphenol extract (WPE).

Investigating the induction of polyphenol biosynthesis in the cultured Cycolocarya paliurus cells and the stimulatory mechanism of co-induction with 5-aminolevulinic acid and salicylic acid

“…All the assembled unigenes were searched against public protein databases, such as NCBI non-redundant protein sequences (NR) and Swiss-Prot for homology comparison. Only those matches with E-value below 10–5 were deemed significant ( Park et al, 2021 ; Jonathan et al, 2022 ). Furthermore, gene function prediction and classification were assigned based on the database of the Gene Ontology (GO) and Eukaryotic Orthologous Groups of proteins (KOG).…”

Antioxidant and Anti-Inflammatory Properties of Walnut Constituents: Focus on Personalized Cancer Prevention and the Microbiome

“…Radical scavenging activities were determined for these phenolic compounds. Additional transcriptomic profiling further ascertained how WPE can rescue gastric cells from H. pylori infection [78]. Multiple chemokinerelated pathways were altered by WPEs, including an array of anti-inflammatory and anti-oxidative genes, providing further evidence for the potential impact of walnut extracts on modulating key signaling pathways that may ultimately contribute to the carcinogenic process present in H. pylori-infected gastric cells [78].…”

“…Additional transcriptomic profiling further ascertained how WPE can rescue gastric cells from H. pylori infection [78]. Multiple chemokinerelated pathways were altered by WPEs, including an array of anti-inflammatory and anti-oxidative genes, providing further evidence for the potential impact of walnut extracts on modulating key signaling pathways that may ultimately contribute to the carcinogenic process present in H. pylori-infected gastric cells [78]. Nunez-Sanchez et al [79] tested EA and its microbially-derived metabolites, the urolithins, at concentrations predicted in colon tissue following ingestion of EA-rich foods, based upon the established urolithin 'metabotypes' [52].…”

Polyphenol Mechanisms against Gastric Cancer and Their Interactions with Gut Microbiota: A Review

“…The inhibitory function of resveratrol and epicatechin on IL-8 is probably related to their inhibitory activity on the NF-κB pathway, which downregulates the expression level of IL-8. Research has found that Walnut polyphenol extracts prevent Hp -related tumor growth by inhibiting STAT3 phosphorylation and nuclear translocation in gastric mucosal cells [ 63 ], and reversing precancerous atrophic gastritis [ 64 ]. Nobiletin has been confirmed to inhibit Hp infection and prevent Hp -mediated GC [ 65 ].…”