Sclerotia formed by citric acid producing strains of Aspergillus niger: Induction and morphological analysis

Ellena, Valeria; Bucchieri, Daniela; Arcalís, Elsa; Sauer, Michael; Steiger, Matthias G.

doi:10.1016/j.funbio.2021.01.008

Cited by 8 publications

(7 citation statements)

References 35 publications

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“…S3), which are an important prerequisite for sexual development in Aspergillus ( 42 , 43 ). The observed putative sclerotia were highly similar in size, structure, and color to those recently characterized from A. niger ( 44 ). However, A. niger sensu stricto has not been reported to have a sexual cycle.…”

Section: Resultssupporting

confidence: 77%

Beyond the Biosynthetic Gene Cluster Paradigm: Genome-Wide Coexpression Networks Connect Clustered and Unclustered Transcription Factors to Secondary Metabolic Pathways

et al. 2021

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Section: Resultssupporting

confidence: 77%

Beyond the Biosynthetic Gene Cluster Paradigm: Genome-Wide Coexpression Networks Connect Clustered and Unclustered Transcription Factors to Secondary Metabolic Pathways

et al. 2021

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“…Despite showing this unusual orientation, the presence of a 1:1 MAT1-1:MAT1-2 ratio among 24 randomly selected natural A. niger isolates is an important observation, which suggests that sexual reproduction is occurring in this species. Moreover, A. niger was previously shown to be able to form sclerotia [51][52][53][54], important prerequisite for sexual development in closely related species. Therefore, further research should focus on the possibility to e ciently induce a sexual cycle in A. niger.…”

Section: Genetic Comparison Of Mat Loci In Different Aspergilli and Amentioning

confidence: 99%

Genome sequencing of the neotype strain CBS 554.65 reveals the MAT1-2 locus of Aspergillus niger

Ellena

Seekles

Steiger

2021

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Background Aspergillus niger is a ubiquitous filamentous fungus widely employed as a cell factory thanks to its abilities to produce a wide range of organic acids and enzymes. Due to its economic importance and its role as model organism to study fungal fermentation, its genome was one of the first Aspergillus genomes to be sequenced in 2007. Nowadays, the genome sequences of at least five other A. niger strains are available. These, however, do not include the neotype strain CBS 554.65. Results In this study, the genome of CBS 554.65 was sequenced with PacBio. A high-quality nuclear genome sequence consisting of 17 contigs with a N50 value of 4.07 Mbp was obtained. The sequencing covered all the 8 centromeric regions of the chromosomes. In addition, a complete circular mitochondrial DNA assembly was obtained. In silico analyses revealed the presence of a MAT1-2-1 gene in this genome, contrary to the so far sequenced A. niger strains, which all contain a MAT1-1-1 gene. An alignment at the MAT locus showed a different position of the MAT1-1-1 gene of ATCC 1015 compared to the MAT1-2-1 gene of CBS 554.65, relative to the surrounding genes. In addition, 24 other sequenced isolates of A. niger showed a 1:1 ratio of MAT1-1 and MAT1-2 loci. While the genetic organization of the MAT1-2 locus of CBS 554.65 is similar to what is found in other aspergilli, the genetic organization of the MAT1-1 locus is flipped in all sequenced strains. Conclusions This study, besides providing a high-quality genome sequence of an important A. niger strain, suggests the occurrence of genetic flipping or switching events at the MAT1-1 locus of A. niger. These results provide new insights in the mating system of A. niger and could contribute to the investigation and potential discovery of sexuality of this so far asexual fungal species.

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“…Most species in section Nigri are asexual, producing a large number of asexual (mitotic) spores (conidia), however, a few species have been shown to have a sexual state. 7–11 Interestingly, the two mating types needed for sexual reproduction have been found in different Aspergillus section Nigri species. 11,12 A large number of species can produce sclerotia (large, firm, frequently rounded masses of hyphae serving as propagules of new growth or as long-lasting resting structures) under certain conditions, 13–19 and sclerotium production is important as certain indoloterpenes are only produced in the sclerotia, and indoloterpenes may go unnoticed if sclerotia are not formed.…”

Section: Introductionmentioning

confidence: 99%

“…7–11 Interestingly, the two mating types needed for sexual reproduction have been found in different Aspergillus section Nigri species. 11,12 A large number of species can produce sclerotia (large, firm, frequently rounded masses of hyphae serving as propagules of new growth or as long-lasting resting structures) under certain conditions, 13–19 and sclerotium production is important as certain indoloterpenes are only produced in the sclerotia, and indoloterpenes may go unnoticed if sclerotia are not formed. 8 Section Nigri is most closely related to sections Flavi , Candidi , and Circumdati in subgenus Circumdati of Aspergillus according to phylogeny, 19 morphology and secondary metabolite production.…”

Section: Introductionmentioning

confidence: 99%

Current status of secondary metabolite pathways linked to their related biosynthetic gene clusters in Aspergillus section Nigri

et al. 2023

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Sclerotia formed by citric acid producing strains of Aspergillus niger: Induction and morphological analysis

Cited by 8 publications

References 35 publications

Beyond the Biosynthetic Gene Cluster Paradigm: Genome-Wide Coexpression Networks Connect Clustered and Unclustered Transcription Factors to Secondary Metabolic Pathways

Beyond the Biosynthetic Gene Cluster Paradigm: Genome-Wide Coexpression Networks Connect Clustered and Unclustered Transcription Factors to Secondary Metabolic Pathways

Genome sequencing of the neotype strain CBS 554.65 reveals the MAT1-2 locus of Aspergillus niger

Current status of secondary metabolite pathways linked to their related biosynthetic gene clusters in Aspergillus section Nigri

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