Gene expression profile and long noncoding RNA analysis in Candida albicans insoluble β-glucan-stimulated CD14+ monocytes and THP-1 cells

Hou, Jun-sheng; He, Yayi; Du, Leilei; Duan, Zhimin; Xu, Chen; Li, Min

doi:10.1016/j.micpath.2021.104963

Cited by 3 publications

(3 citation statements)

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“…P65 and p50 translocate into the nucleus and bind to specific binding sites to initiate the transcriptional process of inflammatory genes [ 134 ]. New evidence found 808 mRNAs and 51 lncRNAs differently expressed both in β-glucan-stimulated CD14 + monocytes and THP-1 cells, and they are involved in the NF-κB-signaling pathway [ 135 ]. We concluded that lncRNAs are likely to suppress the inflammatory response by interfering with the NF-κB signal pathways.…”

Section: Lncrnas As Potential Therapeutic Targets For Hdts Against ...mentioning

confidence: 99%

LncRNA: A Potential Target for Host-Directed Therapy of Candida Infection

Wang

Chen

et al. 2022

Pharmaceutics

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Despite various drugs work against Candida, candidiasis represents clinical management challenges worldwide due to the rising incidence and recurrence rate, as well as epidemics, of new drug-resistant pathogens. Recent insights into interactions between Candida and hosts contribute to exploring novel therapeutic strategies, termed host-directed therapies (HDTs). HDTs are viable adjuncts with good efficacy for the existing standard antifungal regimens. However, HDTs induce other response unintendedly, thus requiring molecular targets with highly specificity. Long noncoding RNAs (lncRNAs) with highly specific expression patterns could affect biological processes, including the immune response. Herein, this review will summarize recent advances of HDTs based on the Candida–host interaction. Especially, the findings and application strategies of lncRNAs related to the host response are emphasized. We propose it is feasible to target lncRNAs to modulate the host defense during Candida infection, which provides a new perspective in identifying options of HDTs for candidiasis.

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Section: Lncrnas As Potential Therapeutic Targets For Hdts Against ...mentioning

confidence: 99%

LncRNA: A Potential Target for Host-Directed Therapy of Candida Infection

Wang

Chen

et al. 2022

Pharmaceutics

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“…Human lncRNAs RP11-588G21.2 and RP11-394I13.1 have been identified and proposed as potential biomarkers for C. albicans infection [12]. Hou et al [13] recently reported the lncRNA lnc-CCL3L3-1:1 as potentially related to the human inflammatory response to β-glucan, while analyzing gene expression of CD14+ monocytes stimulated by C. albicans cell wall polysaccharide.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis of Long Non-Coding RNA during Candida albicans Infection

Gonçalves

Poloni

Dorn

2023

Genes

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Candida albicans is one of the most commonly found species in fungal infections. Due to its clinical importance, molecular aspects of the host immune defense against the fungus are of interest to biomedical sciences. Long non-coding RNAs (lncRNAs) have been investigated in different pathologies and gained widespread attention regarding their role as gene regulators. However, the biological processes in which most lncRNAs perform their function are still unclear. This study investigates the association between lncRNAs with host response to C. albicans using a public RNA-Seq dataset from lung samples of female C57BL/6J wild-type Mus musculus with induced C. albicans infection. The animals were exposed to the fungus for 24 h before sample collection. We selected lncRNAs and protein-coding genes related to the host immune response by combining the results from different computational approaches used for gene selection: differential expression gene analysis, co-expression genes network analysis, and machine learning-based gene selection. Using a guilt by association strategy, we inferred connections between 41 lncRNAs and 25 biological processes. Our results indicated that nine up-regulated lncRNAs were associated with biological processes derived from the response to wounding: 1200007C13Rik, 4833418N02Rik, Gm12840, Gm15832, Gm20186, Gm38037, Gm45774, Gm4610, Mir22hg, and Mirt1. Additionally, 29 lncRNAs were related to genes involved in immune response, while 22 lncRNAs were associated with processes related to reactive species production. These results support the participation of lncRNAs during C. albicans infection, and may contribute to new studies investigating lncRNA functions in the immune response.

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“…Immune cell gene expression changes in response to different conditions or stimuli such as infection, ageing, and diseases, including cancer, have revealed relevant new pathways and co-expression networks [1][2][3][4]. Efficient next-generation RNA sequencing platforms have facilitated the ability to perform whole genome expression profiling of individual populations of immune cells [5][6][7][8] as a novel means of searching for patterns of gene expression to aid in the identification of meaningful signals of various disease states.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of an RNAseq-Based Immunogenomic Liquid Biopsy Approach in Early-Stage Prostate Cancer

Neste

Wojno

Henao

et al. 2021

Cells

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The primary objective of this study is to detect biomarkers and develop models that enable the identification of clinically significant prostate cancer and to understand the biologic implications of the genes involved. Peripheral blood samples (1018 patients) were split chronologically into independent training (n = 713) and validation (n = 305) sets. Whole transcriptome RNA sequencing was performed on isolated phagocytic CD14+ and non-phagocytic CD2+ cells and their gene expression levels were used to develop predictive models that correlate to adverse pathologic features. The immune-transcriptomic model with the highest performance for predicting adverse pathology, based on a subtraction of the log-transformed expression signals of the two cell types, displayed an area under the curve (AUC) of the receiver operating characteristic of 0.70. The addition of biomarkers in combination with traditional clinical risk factors (age, serum prostate-specific antigen (PSA), PSA density, race, digital rectal examination (DRE), and family history) enhanced the AUC to 0.91 and 0.83 for the training and validation sets, respectively. The markers identified by this approach uncovered specific pathway associations relevant to (prostate) cancer biology. Increased phagocytic activity in conjunction with cancer-associated (mis-)regulation is also represented by these markers. Differential gene expression of circulating immune cells gives insight into the cellular immune response to early tumor development and immune surveillance.

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Gene expression profile and long noncoding RNA analysis in Candida albicans insoluble β-glucan-stimulated CD14+ monocytes and THP-1 cells

Cited by 3 publications

References 50 publications

LncRNA: A Potential Target for Host-Directed Therapy of Candida Infection

LncRNA: A Potential Target for Host-Directed Therapy of Candida Infection

Transcriptomic Analysis of Long Non-Coding RNA during Candida albicans Infection

Evaluation of an RNAseq-Based Immunogenomic Liquid Biopsy Approach in Early-Stage Prostate Cancer

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