SNHG9, a Papillary Thyroid Cancer Cell Exosome-Enriched lncRNA, Inhibits Cell Autophagy and Promotes Cell Apoptosis of Normal Thyroid Epithelial Cell Nthy-ori-3 Through YBOX3/P21 Pathway

Abstract

Thyroid cancer is the most common type of endocrine malignancy. Although the general prognosis is good, the treatment of advanced disease is still challenging. Exosomes are vesicle units containing specific components that transmit information between cells. In order to explore its role in papillary thyroid cancer (PTC), our study screened exosome enriched lncRNA SNHG9 by lncRNA chip and explored its biological function. We used lncRNA chips combined with bioinformatics analysis to screen lncRNA SNHG9 enriched in exosomes. GO analysis suggested its relationship with autophagy and apoptosis. Quantitative PCR showed SNHG9 was highly expressed in PTC cells and exosomes and its correlation with PTC tumor size was analyzed by clinical characteristics. SNHG9 could inhibit the protective cell autophagy induced by starvation of human normal thyroid epithelial cell line Nthy-ori-3 and promote its apoptosis through PTC cell exosomes. RNA-pull down combined with protein spectrum showed that SNHG9 could interact with YBOX3. Western blot and RNA immunoprecipitation further confirmed their interaction. Western blot showed that SNHG9 could induce degradation of YBOX3, thus interfering with the stability of P21 mRNA and inducing cell apoptosis. In conclusion, our study identified SNHG9 as a PTC cell exosome-enriched lncRNA. SNHG9 could inhibit cell autophagy and promote apoptosis of Nthy-ori-3 cell through YBOX3/P21 pathway.

Exosome-mediated lncRNA SND1-IT1 from gastric cancer cells enhances malignant transformation of gastric mucosa cells via up-regulating SNAIL1

“…1C). It has been reported that cancer cells can regulate the malignant progression of normal epithelial cells through secreting exosomes [16]. Hence we speculated that GC cells can influence GES-1 cells via secreting exosomes.…”

Long noncoding RNA NONMMUT015745 inhibits doxorubicin-mediated cardiomyocyte apoptosis by regulating Rab2A-p53 axis

“…Moreover, RNA pulldown and MS analyses in NMVCs identified Rab2A as a protein that combined with lnc5745. Previous studies have demonstrated that lncRNAs may participate in the stabilization and degradation of targeted proteins, thereby regulating their expression level and functions [ 51 , 52 ]. We found that lnc5745 functions as an upstream regulator of Rab2A and negatively regulates Rab2A expression.…”

Role of Extracellular Vesicles in Thyroid Physiology and Diseases: Implications for Diagnosis and Treatment

“…SNHG9, a lncRNA overexpressed in PTC tissues compared to adjacent thyroid specimens, is also overexpressed in the PTC cell lines, TPC-1 and K1, as compared to Nthy-ori 3-1. It was shown that SNHG9 is enriched in EVs from PTC cells and that the EV-mediated transfer of SNHG9 inhibited autophagy and promoted apoptosis of Nthy-ori 3-1 through interaction and negative regulation of YBOX3 and P21 [ 32 ].…”