Molecular Characterization of All-Stage and Adult-Plant Resistance Loci Against Powdery Mildew in Winter Wheat Cultivar Liangxing 99 Using BSR-Seq Technology

Shi, Xiaohan; Wu, Peipei; Hu, Jinghuang; Qiu, Dan; Qu, Yunfeng; Li, Yahui; Liu, Yi; Gebremariam, Tesfay Gebrekirstos; Xie, Jingzhong; Wu, Qiuhong; Zhang, Hongjun; Liu, Hongwei; Yang, Li; Sun, Guo-Zhong; Zhou, Yang; Liu, Zhiyong; Li, Hongjie

doi:10.1094/pdis-03-21-0664-re

Cited by 11 publications

(3 citation statements)

References 50 publications

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“…Our field resistance screening results showed that LX99 was highly resistant (immune) to S. mosellana, which was consistent with the findings in previous studies. − LX99 has been demonstrated to be highly resistant to powdery mildew at both the seedling and adult plant stages. − As LX99 is a high-quality wheat variety with disease and insect resistance, the understanding of its resistance mechanism to S. mosellana will contribute to the breeding of excellent wheat varieties.…”

Section: Discussionsupporting

confidence: 89%

Transcriptomic and Metabolomic Analysis of Wheat Kernels in Response to the Feeding of Orange Wheat Blossom Midges (Sitodiplosis mosellana) in the Field

Wang

Liu

et al. 2022

J. Agric. Food Chem.

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The orange wheat blossom midge (Sitodiplosis mosellana Gehin) is an insect pest that feeds on wheat (Triticum aestivum L.). The resistance mechanisms of wheat to S. mosellana infestation are largely unknown. In this study, the wheat varieties LX99 and 6218 were identified as highly resistant and susceptible, respectively, via field investigations conducted over two consecutive years. Morphological and microstructural observations of mature wheat kernels following S. mosellana infestation revealed that the degree of cell structure damage in resistant LX99 grains was less than that in susceptible 6218 grains. Transcriptomic and metabolomic analyses of seeds following S. mosellana feeding showed that the differentially expressed genes and differentially accumulated metabolites from LX99 were mostly enriched in several primary and secondary metabolic pathways, including phenylpropanoid biosynthesis, flavonoid biosynthesis, and phenylalanine biosynthesis. Additionally, phenylpropanoid-and flavonoid-related gene expression was significantly upregulated following S. mosellana infestation in LX99 relative to that in 6218. Some metabolites involved in phenylpropanoid/flavonoid pathways, such as cinnamic acid, coumarin, epigallocatechin, and naringenin, were only induced in infested LX99 kernels. These results suggest that phenylpropanoid/flavonoid pathways play important roles in wheat kernel resistance to S. mosellana attack and provide useful insights for the breeding and utilization of resistant varieties.

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Section: Discussionsupporting

confidence: 89%

Transcriptomic and Metabolomic Analysis of Wheat Kernels in Response to the Feeding of Orange Wheat Blossom Midges (Sitodiplosis mosellana) in the Field

Wang

Liu

et al. 2022

J. Agric. Food Chem.

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“…Four QTL were responsible for the slow-mildewing resistance. Cultivars such as Liangxing 99, often have both ASR and APR (Zhao et al 2013;Shi et al 2021). The high-level resistance conferred by the ASR genes plays a signi cant extension role in the early stage of cultivar use.…”

Section: Application Of Slow-mildewing Resistance In Breedingmentioning

confidence: 99%

Molecular identification and validation of four stable QTL for adult-plant resistance to powdery mildew in Chinese wheat cultivar Bainong 64

Xu,

Sun,

et al. 2023

Theor Appl Genet

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Wheat powdery mildew, caused by Blumeria graminis f. sp tritici (Bgt), is a devastating fungal disease. Seedlings of Chinese winter wheat cultivar Bainong 64 are susceptible to Bgt but adult plants have maintained slow-mildewing resistance since it was released in 1996. A population of 171 recombinant inbred lines (RILs) developed from cross Jingshuang 16/Bainong 64 (JS16/BN64) was used to dissect genetic components of powdery mildew resistance. A genetic map comprising 5,383 polymorphic markers was constructed using the 15K SNP chip and kompetitive allele-speci c PCR (KASP) markers.Composite interval mapping identi ed four stable QTL on chromosome arms 1DL, 2BS, 2DL, and 6BL in all ve environments and their favorable alleles were inherited from BN64. The QTL accounted for 8.3%, 13.8%, 14.4%, and 9.0% of the total phenotypic variances in maximum, respectively. QPmjbr.caas-1DL,situated about 22 Mb from centromere, is probably a new QTL. QPmjbr.caas-2DL located near the end of arm 2DL. Using genetic maps populated with KASP markers, QPmjbr.caas-2BS and QPmjbr.caas-6BL were ne mapped to a 1.8 cM genetic intervals spanning 13.6 Mb (76.0-89.6 Mb) and 1.7 cM and 4.9 Mb (659.9-664.8 Mb), respectively. The four QTL were all independent of stripe rust and leaf rust resistance and were validated for slow-mildewing in another RIL population related to BN64 and a cultivar panel using representative KASP markers spanning each QTL. Since BN64 has been a leading cultivar and an important breeding parent, the QTL and markers reported in this study will be useful for markerassisted selection of slow-mildewing resistance. Key MessageFour stable QTL for slow-mildewing were identi ed on chromosome arms 1DL, 2BS, 2DL and 6BL in the widely grown Chinese wheat cultivar Bainong 64. These QTL had no effect on response to stripe rust or leaf rust.

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“…BSA has been widely used in wheat genetic mapping, and it is one of the effective methods to rapidly obtain target genes [18,49,50]. For species with large and complex genomes like wheat, it is a huge and laborious project to complete genome sequencing.…”

Section: The Feasibility Of Qtl Mapping Of Quantitative Traits Using ...mentioning

confidence: 99%

Identification and validation of a major QTL for kernel length in bread wheat based on two F3 biparental populations

Xie

Liu

et al. 2022

BMC Genomics

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Background High yield and quality are essential goals of wheat (Triticum aestivum L.) breeding. Kernel length (KL), as a main component of kernel size, can indirectly change kernel weight and then affects yield. Identification and utilization of excellent loci in wheat genetic resources is of great significance for cultivating high yield and quality wheat. Genetic identification of loci for KL has been performed mainly through genome-wide association study in natural populations or QTL mapping based on genetic linkage map in high generation populations. Results In this study, an F3 biparental population derived from the cross between an EMS mutant BLS1 selected from an EMS-induced wheat genotype LJ2135 (derived from the hybrid progeny of a spelt wheat (T. spelta L.) and a common wheat) mutant bank and a local breeding line 99E18 was used to rapidly identify loci controlling KL based on Bulked Segregant Analysis (BSA) and the wheat 660 K single-nucleotide polymorphism (SNP) array. The highest ratio of polymorphic SNPs was located on chromosome 4A. Linkage map analysis showed that 33 Kompetitive Allele Specific PCR markers were linked to the QTL for KL (Qkl.sicau-BLE18-4A) identified in three environments as well as the best linear unbiased prediction (BLUP) dataset. This QTL explained 10.87—19.30% of the phenotypic variation. Its effect was successfully confirmed in another F3 population with the two flanking markers KASP-AX-111536305 and KASP-AX-110174441. Compared with previous studies and given that the of BLS1 has the genetic background of spelt wheat, the major QTL was likely a new one. A few of predicted genes related to regulation of kernel development were identified in the interval of the detected QTL. Conclusion A major, novel and stable QTL (Qkl.sicau-BLE18-4A) for KL was identified and verified in two F3 biparental populations across three environments. Significant relationships among KL, kernel width (KW) and thousand kernel weight (TKW) were identified. Four predicted genes related to kernel growth regulation were detected in the interval of Qkl.sicau-BLE18-4A. Furthermore, this study laid foundation on subsequent fine mapping work and provided a possibility for breeding of elite wheat varieties.

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Molecular Characterization of All-Stage and Adult-Plant Resistance Loci Against Powdery Mildew in Winter Wheat Cultivar Liangxing 99 Using BSR-Seq Technology

Cited by 11 publications

References 50 publications

Transcriptomic and Metabolomic Analysis of Wheat Kernels in Response to the Feeding of Orange Wheat Blossom Midges (Sitodiplosis mosellana) in the Field

Transcriptomic and Metabolomic Analysis of Wheat Kernels in Response to the Feeding of Orange Wheat Blossom Midges (Sitodiplosis mosellana) in the Field

Molecular identification and validation of four stable QTL for adult-plant resistance to powdery mildew in Chinese wheat cultivar Bainong 64

Identification and validation of a major QTL for kernel length in bread wheat based on two F3 biparental populations

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