Purification of TET Proteins

Huang, Zhijun; Yu, Jeesuk; Johnson, Jennifer; Jin, Seung-Gi; Pfeifer, Gerd P.

doi:10.1007/978-1-0716-1294-1_13

Cited by 2 publications

(2 citation statements)

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“…The International Agency for Research on Cancer (IARC) of the World Health Organization has listed BaP as a class I human carcinogen [ 92 , 143 ], Goedtke, Sprenger et al [ 46 ], and accumulated studies have revealed a close relationship between BaP and cancers in respiratory system [ 120 ], digestive system [ 44 ], reproductive system [ 121 ] etc. The toxicity of BaP to cells is mainly causing DNA damage [ 84 ] and oxidative stress [ 64 , 65 ] by an increase in reactive oxygen species (ROS) production. BaP requires metabolic activation before reaction with DNA, and its metabolite benzo(a)pyrene-trans-7,8-dihydrodiol-9,10-epoxide (BPDE) induced genotoxicity by forming BPDE-DNA adduct [ 80 , 106 ], which showing mutagenic and carcinogenic potential in cells [ 85 ].…”

Section: Introductionmentioning

confidence: 99%

“…TET proteins belong to α-ketoglutarate- and Fe 2+ -dependent dioxygenases, and Tet1, Tet2 and Tet3 involved in this family [ 26 ]. Tet1 and Tet2 mainly regulates the level of 5-hydroxymethylcytosine (5hmC) in the promoter region of primordial germ cell (PGCs) genes or 5hmC inside PGCs gene, while Tet3-mediates paternal active DNA demethylation [ 7 , 64 , 65 , 89 , 94 , 134 , 161 ]. Active DNA demethylation occurs under the catalysis by TET proteins, which sequentially oxidize 5-methylcytosine (5mC) to 5hmC, then to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) [ 38 , 104 , 126 , 147 ].…”

Section: Introductionmentioning

confidence: 99%

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Dynamic changes of DNA methylation induced by benzo(a)pyrene in cancer

et al. 2023

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Benzo(a)pyrene (BaP), the earliest and most significant carcinogen among polycyclic aromatic hydrocarbons (PAHs), has been found in foods, tobacco smoke, and automobiles exhaust, etc. Exposure to BaP induced DNA damage directly, or oxidative stress-related damage, resulting in cell apoptosis and carcinogenesis in human respiratory system, digestive system, reproductive system, etc. Moreover, BaP triggered genome-wide epigenetic alterations by methylation, which might cause disturbances in regulation of gene expression, and thereby induced cancer. It has been proved that BaP reduced genome-wide DNA methylation, and activated proto-oncogene by hypomethylation in the promoter region, but silenced tumor suppressor genes by promoter hypermethylation, resulting in cancer initiation and progression. Here we summarized the changes in DNA methylation in BaP exposure, and revealed the methylation of DNA plays a role in cancer development. Graphical Abstract

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