Cytoskeletal players in single-cell branching morphogenesis

Ricolo, Delia; Castro-Ribera, Judith; Araújo, Sofia J.

doi:10.1016/j.ydbio.2021.05.001

Cited by 7 publications

(5 citation statements)

References 113 publications

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“…By focusing on the following four structural features, we obtained 11 distinct motor domain structures from phi and open dynein: (1) the pocket conformations of AAA1, (2) and of AAA3, (3) the linker position, and (4) the buttress-stalk conformations (Figure S1, S3) . By primarily focusing on AAA1 and the overall AAA+ ring arrangement, these 11 structures were further categorized into 9 major states (state-0 to state-8) (Figure 2A) .…”

Section: Resultsmentioning

confidence: 99%

“…Dyneins are large cytoskeletal motor complexes that move toward the minus ends of microtubules (MTs) (1) . Cytoplasmic dynein-1 is the sole cytoplasmic isoform that drives retrograde intracellular cargo transport (2) , organelle positioning (3) , and cellular morphogenesis (4) , while dynein-2 and axonemal dyneins mediate intraflagellar transport (5) and ciliary motility (6) . Unlike the kinesin family which encompasses 45 different types of plus end-directed motors in humans (7) , a single cytoplasmic dynein-1 (dynein) is responsible for transporting diverse cellular cargos including membrane-bound organelles, RNAs, proteins, and viruses (2) .…”

Section: Introductionmentioning

confidence: 99%

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Cryo-EM Reveals the Mechanochemical Cycle of Reactive Full-length Human Dynein-1

Chai,

Yang,

Geohring

et al. 2024

Preprint

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Dynein-mediated cargo transport plays pivotal roles in a variety of essential cellular activities, including vesicle trafficking, organelle and nucleus positioning, mitosis and morphogenesis. Central to all these cellular processes is dynein's ATP-dependent mechanochemical cycle. Here, we perform a systematic cryo-electron microscopic investigation of the conformational landscape of full-length human dynein-1 actively undergoing nucleotide-induced conformational changes, both on and off microtubules. Our approach reveals over 30 high-resolution structures, categorized into nine distinct states of the AAA+ motor ring. This provides a dynamic and comprehensive view of dynein throughout its mechanochemical cycle. The novel intermediate states unveil a series of important mechanistic insights into dynein function, including a 'backdoor' phosphate release mechanism that coordinates linker straightening, a stepwise structural basis for the bidirectional communications between ATP binding/hydrolysis and microtubule-binding affinity, and elucidate the mechanism by which microtubule binding increases ATPase activity. Our findings also lead to a substantially revised model for the force-generating power stroke, clarifying the means by which dynein achieves its unidirectional stepping on microtubules. These results substantially enhance our understanding of dynein function and provide a more complete model of its mechanochemical cycle.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cryo-EM Reveals the Mechanochemical Cycle of Reactive Full-length Human Dynein-1

Chai,

Yang,

Geohring

et al. 2024

Preprint

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“…At the beginning of neuronal morphogenesis, before polarization, microtubules emanate through the soma from the active centrosome. In the early stages of neuronal polarization, the axon is specified from the branch closest to the active centrosome but at later stages, the centrosome is inactive [51]. The centrosome's position in the cell varies during neuron development.…”

Section: Constancy Of Architecture and Tissue-specific Functioning Fe...mentioning

confidence: 99%

“…Centrosome activity is critical during the first steps of subcellular lumen formation. But later, when the lumen is already formed, centrosomes become inactive and non-centrosomal microtubule polymerization sites are switched on [51].…”

Section: Constancy Of Architecture and Tissue-specific Functioning Fe...mentioning

confidence: 99%

The Endothelial Centrosome: Specific Features and Functional Significance for Endothelial Cell Activity and Barrier Maintenance

Shakhov,

Churkina,

Kotlobay

et al. 2023

IJMS

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This review summarizes information about the specific features that are characteristic of the centrosome and its relationship with the cell function of highly specialized cells, such as endotheliocytes. It is based on data from other researchers and our own long-term experience. The participation of the centrosome in the functional activity of these cells, including its involvement in the performance of the main barrier function of the endothelium, is discussed. According to modern concepts, the centrosome is a multifunctional complex and an integral element of a living cell; the functions of which are not limited only to the ability to polymerize microtubules. The location of the centrosome near the center of the interphase cell, the concentration of various regulatory proteins in it, the organization of the centrosome radial system of microtubules through which intracellular transport is carried out by motor proteins and the involvement of the centrosome in the process of the perception of the external signals and their transmission make this cellular structure a universal regulatory and distribution center, controlling the entire dynamic morphology of an animal cell. Drawing from modern data on the tissue-specific features of the centrosome’s structure, we discuss the direct involvement of the centrosome in the performance of functions by specialized cells.

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“…Finally, we point readers to other reviews that elaborate on specific aspects related to this work (Table 2). Chaudhuri et al, 2020;Khalilgharibi and Mao, 2021;Walma and Yamada, 2020;Fidler et al, 2018;Pastor-Pareja, 2020 Cell-cell andcell-ECM interactions Bachir et al, 2017;Dzamba and DeSimone, 2018;Klapholz and Brown, 2017;Moreno-Layseca et al, 2019;Perez-Vale andPeifer, 2020 Actin-MT interactions Dogterom andKoenderink, 2019;Voelzmann et al, 2017Biomechanics in morphogenesis Gilmour et al, 2017Kindberg et al, 2020;Kirby and Lammerding, 2018;Molnar and Labouesse, 2021;Tsata andBeis, 2020 Microtubules Muroyama andLechler, 2017;Röper, 2020Actin cytoskeleton Alekhina et al, 2017Boiero Sanders et al, 2020;Miao and Blankenship, 2020;Rottner et al, 2017 Tracheal development andbranching Hayashi andKondo, 2018;Öztürk-Çolak et al, 2016a;Ricolo et al, 2021 Dorsal closure Hayes and Solon, 2017;Kiehart et al, 2017…”

Section: Concluding Remarks and Open Questionsmentioning

confidence: 99%

Crosstalk between basal extracellular matrix adhesion and building of apical architecture during morphogenesis

Barrera-Velázquez

Ríos-Barrera

2021

Biology Open

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Tissues build complex structures like lumens and microvilli to carry out their functions. Most of the mechanisms used to build these structures rely on cells remodelling their apical plasma membranes, which ultimately constitute the specialised compartments. In addition to apical remodelling, these shape changes also depend on the proper attachment of the basal plasma membrane to the extracellular matrix (ECM). The ECM provides cues to establish apicobasal polarity, and it also transduces forces that allow apical remodelling. However, physical crosstalk mechanisms between basal ECM attachment and the apical plasma membrane remain understudied, and the ones described so far are very diverse, which highlights the importance of identifying the general principles. Here, we review apicobasal crosstalk of two well-established models of membrane remodelling taking place during Drosophila melanogaster embryogenesis: amnioserosa cell shape oscillations during dorsal closure and subcellular tube formation in tracheal cells. We discuss how anchoring to the basal ECM affects apical architecture and the mechanisms that mediate these interactions. We analyse this knowledge under the scope of other morphogenetic processes and discuss what aspects of apicobasal crosstalk may represent widespread phenomena and which ones are used to build subsets of specialised compartments.

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Cytoskeletal players in single-cell branching morphogenesis

Cited by 7 publications

References 113 publications

Cryo-EM Reveals the Mechanochemical Cycle of Reactive Full-length Human Dynein-1

Cryo-EM Reveals the Mechanochemical Cycle of Reactive Full-length Human Dynein-1

The Endothelial Centrosome: Specific Features and Functional Significance for Endothelial Cell Activity and Barrier Maintenance

Crosstalk between basal extracellular matrix adhesion and building of apical architecture during morphogenesis

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