Thermotolerance and Cellulolytic Activity of Fungi Isolated from Soils/Waste Materials in the Industrial Region of Nigeria

Akpomie, Olubunmi O.; Okonkwo, Kosisochukwu E; Gbemre, Aghogho C; Akpomie, Kovo G.; Ghosh, Soumya; Ahmadi, Shahin; Banach, Artur

doi:10.1007/s00284-021-02528-3

Cited by 3 publications

(1 citation statement)

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“…Thermophilic fungi are ubiquitous, widespread over 5,000 identified species that are isolated from various types of substrates and live in several environments such as aquatic sediments, waterlogged grasslands, dungs, industrial waste, mushroom compost, vegetable compost and municipal garbage (de Oliveira et al, 2015;Akpomie et al, 2021). The abundance of carbon rich microhabitats such as lignocellulosic wastes in temperate, dry, and tropical ecosystems, in daily temperature and moisture enhance thermophiles growth and dispersion (Maheshwari et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Cellulolytic activities of thermophilic Thermoascus aurantiacus MW559792 and Mycothermus thermophilus MZ723073.

EL-Morsy,

. El-Metwally,

Mohesien

et al. 2023

Scientific Journal for Damietta Faculty of Science

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Seven thermophilic fungal species were isolated from various thermogenic habitats on modified Czapek's Dox agar medium with CMC replacing glucose and identified based on their morphological characters and sequence homology of their rRNA gene sequences. Congo red test was used to display their cellulolytic activity. The cellulolytic activities (FPase, CMCase and βglucosidase) of five selected species were performed on solid state fermentation (SSF). Thermoascus aurantiacus MW559792 and Mycothermus thermophilus MZ723073 were selected for optimization experiments based on their maximum productivity of cellulases. Culture conditions were optimized under SSF experiment. Of the five lignocellulosic wastes used as carbon sources, rice straw was the most favorable for maximum production of FPase (1.78, 1.45 U/g), CMCase (6.18, 4.09 U/g) and β-glucosidase (31.93, 16.23 U/g) from T. aurantiacus and My. thermopilus, respectively. The suitability of the lignocellulosic substrates was in the following order: rice straw> maize straw > wheat straw>corn cob> rice hulls in case of T. aurantiaccus, and rice straw> wheat straw>maize straw>corn cob>rice hulls in case of My. thermophilus. Peptone was the most suitable nitrogen source for maximum FPase (1.88, 1.46 U/g), CMCase (7.02, 4.89 U/g) and β-glucosidase (28.60, 19.91 U/g) activities by T. aurantiacus and My. thermophilus respectively. Nitrogen sources availability was in the following order: Peptone > Yeast Extract>(NH₄) ₂SO₄> Beef Extract> NH4NO3> NaNO3> KNO3. The maximum cellulolytic activities of T. aurantiacus and My. thermophilus were also achieved at pH 5, 50°C and 45°C for 4 to 6 days of incubation respectively. On that bases T. aurantiacus MW559792 and My. thermophilus MZ723073 were suggested to use industrially for bioconversion of lignocellulosic wastes at the saccharification stage.

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Section: Introductionmentioning

confidence: 99%