340. Cellular Uptake and Subsequent Intracellular of R8-Liposomes Introduced at Low Temperature

DNA–Lipid Amphiphiles for Drug and Gene Therapy

“…92 Figure 14.9 shows an example of intracellular traffi cking of lipoplexes being visualized using LCSM; the plasma membrane of target cell was stained with PKH67 (green fl uorescent dye), and the lipoplexes were labeled with red fl uorescent dye, so the cellular uptake and nuclear entry of lipoplex can be observed. 93 For DNA delivery, it is useful to employ a plasmid that encodes reporter genes, such as green fl uorescence protein ( GFP ) or luciferase, which allows the transfection effi ciency to be measured using simple assays. For siRNA delivery, it may be useful to employ cell lines that stably express reporter gene GFP and luciferase, so that the transfection effi ciency and the degree of gene knockdown can be measured easily.…”