An integrated self-powered 3D printed sample concentrator for highly sensitive molecular detection of HIV in whole blood at the point of care

Kadimisetty, Karteek; Yin, Kun; Roche, Aoife M.; Yi, Yanjie; Bushman, Frederic D.; Collman, Ronald G.; Gross, Robert; Feng, Liang; Liu, Changchun

doi:10.1039/d0an02482a

Cited by 7 publications

(13 citation statements)

References 26 publications

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“…Plasma is the gold-standard specimen for HIV VL quantification. Many efforts have been made to develop methods for plasma separation at the POC. ,− However, many of these methods are complex, have limited plasma separation efficiencies, and/or can only process small blood volumes. In response, we herein report a simple, power-free plasma separation method utilizing a Vivid plasma separation membrane and absorbent material.…”

Section: Resultsmentioning

confidence: 99%

“…Several efforts have been made to develop such a POC HIV VL test. ,− For example, Kadimisetty et al reported a three-dimensional (3D)-printed sample concentrator for plasma separation, HIV virus lysis, and HIV nucleic acid enrichment at the POC . However, the samples had to be transported to centralized laboratories for manual washing and VL quantification using benchtop PCR machines.…”

Section: Introductionmentioning

confidence: 99%

“…4,34−40 For example, Kadimisetty et al reported a threedimensional (3D)-printed sample concentrator for plasma separation, HIV virus lysis, and HIV nucleic acid enrichment at the POC. 35 However, the samples had to be transported to centralized laboratories for manual washing and VL quantification using benchtop PCR machines. Phillips et al reported a POC HIV nucleic acid testing (NAT) device called microRAAD to detect HIV from whole blood using RT-LAMP isothermal amplification.…”

Section: ■ Introductionmentioning

confidence: 99%

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Sensitive and Quantitative Point-of-Care HIV Viral Load Quantification from Blood Using a Power-Free Plasma Separation and Portable Magnetofluidic Polymerase Chain Reaction Instrument

et al. 2022

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Point-of-care (POC) HIV viral load (VL) tests are needed to enhance access to HIV VL testing in low-and middleincome countries (LMICs) and to enable HIV VL self-testing at home, which in turn have the potential to enhance the global management of the disease. While methods based on real-time reverse transcription-polymerase chain reaction (RT-PCR) are highly sensitive and quantitatively accurate, they often require bulky and expensive instruments, making applications at the POC challenging. On the other hand, although methods based on isothermal amplification techniques could be performed using lowcost instruments, they have shown limited quantitative accuracies, i.e., being only semiquantitative. Herein, we present a sensitive and quantitative POC HIV VL quantification method from blood that can be performed using a small power-free three-dimensionalprinted plasma separation device and a portable, low-cost magnetofluidic real-time RT-PCR instrument. The plasma separation device, which is composed of a plasma separation membrane and an absorbent material, demonstrated 96% plasma separation efficiency per 100 μL of whole blood. The plasma solution was then processed in a magnetofluidic cartridge for automated HIV RNA extraction and quantification using the portable instrument, which completed 50 cycles of PCR in 15 min. Using the method, we achieved a limit of detection of 500 HIV RNA copies/mL, which is below the World Health Organization's virological failure threshold, and a good quantitative accuracy. The method has the potential for sensitive and quantitative HIV VL testing at the POC and at home self-testing.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Sensitive and Quantitative Point-of-Care HIV Viral Load Quantification from Blood Using a Power-Free Plasma Separation and Portable Magnetofluidic Polymerase Chain Reaction Instrument

et al. 2022

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“…More recently, impregnated cellulose paper has been developed for molecular testing of various pathogens, including SARS-CoV-2, leishmaniasis, and malaria [47][48][49]. The HIV in separated plasma is directly lysed, and viral nucleic acids are enriched by an integrated, flow-through FTA ® membrane in the concentrator, which enables nucleic acid preparation with high efficiency [50] (Fig. 2c).…”

Section: Porous Materials-based Strategiesmentioning

confidence: 99%

“…b Centrifugal microdevice for dSPE of nucleic acids from buccal swabs (adapted from [39]). c Self-powered integrated sample concentrator using FTA ® card (adapted from [50]). d Chitosan-modified Fusion 5 filter paper (adapted from [51]).…”

Section: Pcrmentioning

confidence: 99%

Microfluidics-based strategies for molecular diagnostics of infectious diseases

Wang

Hong

et al. 2022

Military Med Res

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Traditional diagnostic strategies for infectious disease detection require benchtop instruments that are inappropriate for point-of-care testing (POCT). Emerging microfluidics, a highly miniaturized, automatic, and integrated technology, are a potential substitute for traditional methods in performing rapid, low-cost, accurate, and on-site diagnoses. Molecular diagnostics are widely used in microfluidic devices as the most effective approaches for pathogen detection. This review summarizes the latest advances in microfluidics-based molecular diagnostics for infectious diseases from academic perspectives and industrial outlooks. First, we introduce the typical on-chip nucleic acid processes, including sample preprocessing, amplification, and signal read-out. Then, four categories of microfluidic platforms are compared with respect to features, merits, and demerits. We further discuss application of the digital assay in absolute nucleic acid quantification. Both the classic and recent microfluidics-based commercial molecular diagnostic devices are summarized as proof of the current market status. Finally, we propose future directions for microfluidics-based infectious disease diagnosis.

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Rapid and portable quantification of HIV RNA via a smartphone-enabled digital CRISPR device and deep learning

Ngo,

Akarapipad,

Lee

et al. 2024

Sensors and Actuators Reports

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An integrated self-powered 3D printed sample concentrator for highly sensitive molecular detection of HIV in whole blood at the point of care

Abstract: Rapid and efficient biological sample preparation and pretreatment are crucial for highly sensitive, reliable and reproducible molecular detection of infectious diseases. Herein, we report a self-powered, integrated sample concentrator (SPISC)...

Cited by 7 publications

References 26 publications

Sensitive and Quantitative Point-of-Care HIV Viral Load Quantification from Blood Using a Power-Free Plasma Separation and Portable Magnetofluidic Polymerase Chain Reaction Instrument

Sensitive and Quantitative Point-of-Care HIV Viral Load Quantification from Blood Using a Power-Free Plasma Separation and Portable Magnetofluidic Polymerase Chain Reaction Instrument

Microfluidics-based strategies for molecular diagnostics of infectious diseases

Rapid and portable quantification of HIV RNA via a smartphone-enabled digital CRISPR device and deep learning

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