Strain and host-cell dependent role of type-1 fimbriae in the adherence phenotype of super-shed Escherichia coli O157:H7

Katani, Robab; Kudva, Indira T.; Srinivasan, Sreenidhi; Stasko, Judith B.; Schilling, Megan A.; Cote, Rebecca; DebRoy, Chitrita; Arthur, Terrance M.; Sokurenko, Evgeni V.; Kapur, Vivek

doi:10.1016/j.ijmm.2021.151511

Cited by 11 publications

(12 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PCR assay was performed with 30 cycles at 95°C for 40 s, 54°C for 30 s, and 72°C for 30 s. The sequences of the primers used in the experiment are listed in Table 1 . The genes investigated included fim H, 47 a gene related with biofilm formation in E. coli , rh II and las I genes related with biofilm formation in P. aeruginosa . 48 16S rRNA was used as control.…”

Section: Methodsmentioning

confidence: 99%

Enhanced Antibacterial and Anti-Biofilm Activities of Antimicrobial Peptides Modified Silver Nanoparticles

Xu¹,

Li²,

Wang³

et al. 2021

IJN

View full text Add to dashboard Cite

Background:The biofilms could protect bacteria from antibiotics and promote the production of drug-resistant strains, making the bacteria more difficult to be eradicated. Thus, we developed an AMP@PDA@AgNPs nanocomposite, which is formed by modifying silver nanoparticles (AgNPs) with antimicrobial peptides (AMP) modified nanocomposite to destroy biofilm in this study. Methods: The AMP@PDA@AgNPs nanocomposite was prepared with polymerization method and characterized by using ultraviolet-visible (UV-vis) spectroscopy, dynamic light scattering (DLS), Fourier transform-infrared spectroscopy (FT-IR), and transmission electron microscope (TEM). The antibacterial effects of the nanocomposite were investigated by using agar diffusion method and minimum inhibitory concentration (MIC) test. The quantitative analysis of the biofilm formation by the nanocomposite was conducted using crystal violet staining and confocal laser scanning microscope (CLSM). Results: The DLS and TEM analysis showed it was a spherical nanocomposite with 200 nm size and well dispersed . The results of UV-vis and FT-IR confirmed the presence of AMP and AgNPs. The nanocomposite had an excellent biocompatibility at 100 μg/mL. And the AMP@PDA@AgNPs nanocomposite showed superior antimicrobial activity against both Gram-negative (E. coli, P. aeruginosa) and Gram-positive (S. aureus) bacteria than AgNPs or AMP. Importantly, the mRNA expression of biofilm-related genes were decreased under the action of the nanocomposites. Conclusion: An AMP@PDA@AgNPs nanocomposite with good biocompatibility was successfully prepared. The nanocomposite could destruct bacterial biofilms by inhibiting the expression of biofilm-related genes. The synergistic strategy of AMPs and AgNPs could provide a new perspective for the treatment of bacterial infection.

show abstract

Section: Methodsmentioning

confidence: 99%

Enhanced Antibacterial and Anti-Biofilm Activities of Antimicrobial Peptides Modified Silver Nanoparticles

Xu¹,

Li²,

Wang³

et al. 2021

IJN

View full text Add to dashboard Cite

show abstract

“…Expression of nearly all LEE genes is induced by rectal digesta and intimin-Tir interactions promotes E. coli O157:H7 colonization in cattle [155,158,165]. LEE-independent adherence factors include genes located on plasmid pO157, [166], the O-antigen, the flagellar regulatory system (not functional flagella), fimbriae, and pili [48,139 ▪ ,166–171].…”

Section: Ruminant Reservoirmentioning

confidence: 99%

Escherichia coli 0157:H7 virulence factors and the ruminant reservoir

Kolodziejek

Minnich

Hovde

2022

Current Opinion in Infectious Diseases

View full text Add to dashboard Cite

Purpose of reviewThis review updates recent findings about Escherichia coli O157:H7 virulence factors and its bovine reservoir. This Shiga toxin (Stx)-producing E. coli belongs to the Enterohemorrhagic E. coli (EHEC) pathotype causing hemorrhagic colitis. Its low infectious dose makes it an efficient, severe, foodborne pathogen. Although EHEC remains in the intestine, Stx can translocate systemically and is cytotoxic to microvascular endothelial cells, especially in the kidney and brain. Disease can progress to life-threatening hemolytic uremic syndrome (HUS) with hemolytic anemia, acute kidney failure, and thrombocytopenia. Young children, the immunocompromised, and the elderly are at the highest risk for HUS. Healthy ruminants are the major reservoir of EHEC and cattle are the primary source of human exposure.

show abstract

“…The observed upregulation likely enhances the bacterium's capacity to bind to bovine cells, thereby increasing its likelihood of successful colonization. In fact, E. coli O157:H7 type-1 fimbrial genes have been reported to play a role in the attachment of superseded coli O157:H7 to bovine RAJ squamous epithelial cells in a strain-dependent manner [59]. Further studies could elucidate whether this enhanced adhesion contributes to increased colonization in bovines, potentially exploiting the new knowledge to mitigate STEC colonization of the bovine RAJ, thus reducing human foodborne STEC disease.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis of Shiga Toxin-Producing Escherichia coli O157:H7 on Bovine Rectoanal Junction Cells and Human Colonic Epithelial Cells during Initial Adherence

Edison,

Kudva,

Kariyawasam

2023

Microorganisms

Self Cite

View full text Add to dashboard Cite

Shiga toxin-producing Escherichia coli (STEC) are notorious foodborne pathogens, capable of causing severe diarrhea and life-threatening complications in humans. Cattle, acting as both primary reservoirs and asymptomatic carriers of STEC, predominantly harbor the pathogen in their rectoanal junction (RAJ), facilitating its transmission to humans through contaminated food sources. Despite the central role of cattle in STEC transmission, the molecular mechanisms governing STEC’s adaptation in the RAJ of the asymptomatic reservoir host and its subsequent infection of human colonic epithelial cells, resulting in diarrhea, remain largely unexplored. This study aims to uncover these complicated dynamics by focusing on the STEC O157:H7 serotype within two distinct host environments, bovine RAJ cells and human colonic epithelial cells, during initial colonization. We employed comparative transcriptomics analysis to investigate differential gene expression profiles of STEC O157:H7 during interactions with these cell types. STEC O157:H7 was cultured either with bovine RAJ cells or the human colonic epithelial cell line CCD CoN 841 to simulate STEC-epithelial cell interactions within these two host species. High-throughput RNA sequencing revealed 829 and 1939 bacterial genes expressed in RAJ and CCD CoN 841, respectively. After gene filtering, 221 E. coli O157:H7 genes were upregulated during initial adherence to CCD CoN cells and 436 with RAJ cells. Furthermore, 22 genes were uniquely expressed with human cells and 155 genes with bovine cells. Our findings revealed distinct expression patterns of STEC O157:H7 genes involved in virulence, including adherence, metal iron homeostasis, and stress response during its initial adherence (i.e., six hours post-infection) to bovine RAJ cells, as opposed to human colonic epithelial cells. Additionally, the comparative analysis highlighted the potential role of some genes in host adaptation and tissue-specific pathogenicity. These findings shed new light on the potential mechanisms of STEC O157:H7 contributing to colonize the intestinal epithelium during the first six hours of infection, leading to survival and persistence in the bovine reservoir and causing disease in humans.

show abstract

Strain and host-cell dependent role of type-1 fimbriae in the adherence phenotype of super-shed Escherichia coli O157:H7

Cited by 11 publications

References 55 publications

Enhanced Antibacterial and Anti-Biofilm Activities of Antimicrobial Peptides Modified Silver Nanoparticles

Enhanced Antibacterial and Anti-Biofilm Activities of Antimicrobial Peptides Modified Silver Nanoparticles

Escherichia coli 0157:H7 virulence factors and the ruminant reservoir

Comparative Transcriptome Analysis of Shiga Toxin-Producing Escherichia coli O157:H7 on Bovine Rectoanal Junction Cells and Human Colonic Epithelial Cells during Initial Adherence

Contact Info

Product

Resources

About