Retraction Note to: miR-484 suppresses proliferation and epithelial–mesenchymal transition by targeting ZEB1 and SMAD2 in cervical cancer cells

Hu, Yang; Xie, Hong; Liu, Yankun; Liu, Weiying; Liu, Min; Tang, Hua

doi:10.1186/s12935-021-01958-0

Cited by 3 publications

(3 citation statements)

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“…Zhou et al found that miR-200b-3p can target SMAD2 to promote the progression of melanoma ( Zhou et al,2020 ). Hu et al found that miR-484 inhibits the proliferation and epithelial-mesenchymal transition of cervical cancer cells by targeting ZEB1 and SMAD2 ( Hu et al,2021 ).…”

Section: Discussionmentioning

confidence: 99%

Use of miRNA Sequencing to Reveal Hub miRNAs and the Effect of miR-582-3p/SMAD2 in the Progression of Hepatocellular Carcinoma

Zhao

Miao

et al. 2022

Front. Genet.

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Hepatocellular carcinoma is a common tumor with a high fatality rate worldwide, and exploring its pathogenesis and deterioration mechanism is a focus for many researchers. Increasing evidence has shown that miRNAs are involved in the occurrence and progression of a variety of cancers, including hepatocellular carcinoma. Therefore, this study mainly aimed identify key miRNAs related to hepatocellular carcinoma and explore their potential functions and clinical significance. In this study, we performed miRNA sequencing on three pairs of hepatocellular carcinoma tissue samples and screened 26 differentially expressed miRNAs. Then 2 key miRNAs (miR-139-5p and miR-582-3p) were screened by Kaplan-Meier curve analysis, Cox multivariate analysis and qPCR methods. The expression of miR-582-3p was positively correlated with clinicopathological parameters in patients with hepatocellular carcinoma. Subsequently, miRwalk and starbase were used to predict the target genes of key miRNAs, and then the key pairs miR-582-3p/SMAD2 identified by WGCNA, PPI, qPCR and Pearson correlation analysis. Finally, a dual luciferase experiment, the rescue-of-function experiment and qPCR confirmed that miR-582-3p directly targets SMAD2 and regulates the proliferation, migration and invasion of HepG2 cells by targeting SMAD2. At the same time, interference with SMAD2 can influence the effect of miR-582-3p on HepG2 cells. In conclusion, our findings confirm that miR-582-3p is an independent factor for the prognosis of hepatocellular carcinoma patients, and can regulate the progression of hepatocellular carcinoma cells by targeting SMAD2.

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Section: Discussionmentioning

confidence: 99%

Use of miRNA Sequencing to Reveal Hub miRNAs and the Effect of miR-582-3p/SMAD2 in the Progression of Hepatocellular Carcinoma

Zhao

Miao

et al. 2022

Front. Genet.

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“…[44] Herein, based on bioinformatic analyses, RNA pull-down, and luciferase reporter assays, we identi ed that circFOXK2 contained miR-484 response elements and could speci cally sponge to miR-484 that has been found to act as a tumor suppressor in various cancers, including cervical cancer and colorectal cancer. [45,46] In the pancreatic ductal adenocarcinoma (PDCA), Li et al reported that miR-484 suppresses PDCA growth via reducing Yes-associated protein (YAP) expression. [47] In HCC, we showed that Fis1 and its downstream mitochondrial ssion as a target of miR-484.…”

Section: Discussionmentioning

confidence: 99%

CircFOXK2 Functions as a Protein Encode and a microRNA Sponge to Promote Hepatocellular Carcinoma Progression and Lead a Poor Clinical Prognosis via Regulating the Warburg Effect

Zheng

Pan

et al. 2022

Preprint

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Background The Warburg effect is essential for tumor progression, metastasis, and drug resistance, causing poor clinical outcomes in patients with hepatocellular carcinoma (HCC). Circular RNAs (circRNAs) have emerged as important regulators in HCC development and progression. However, limited circRNAs involved in the Warburg effect of HCC have been investigated. Herein, we aimed to explore the contribution of a novel circRNA that derived from the FOXK2 gene locus, circFOXK2, to the reprogramming of glucose metabolism of HCC. Methods Firstly, we constructed divergent primers to identify 14 circRNAs originated from FOXK2 gene followed by investigating their differential expression between HCC specimens and the adjacent normal tissues (ANTs), and circFOXK2 (has_circ_0000817) was screened for further research. Next, the clinical significance of circFOXK2 for the patients with HCC was evaluated, coupled with evaluating its onco-promoting activity and the affection to the Warburg effect in both HCC cell lines and animal xenograft. Finally, the molecular mechanisms underlying circFOXK2 regulating the Warburg effect of HCC were explored. Results We showed that circFOXK2 was aberrantly upregulation in HCC tissues, and its expression was positively correlated with poor clinical outcomes in patients receiving radical hepatectomy. And silenced circFOXK2 remarkably suppressed the progression of HCC both in vitro and in vivo. Mechanistically, we unveiled that circFOXK2 not only encoded a novel protein, FOXK2-142aa, to promote LDHA phosphorylation but also inactivated miR-484 and subsequently promoted Fis1 expression leading to mitochondrial fission, which activate the Warburg effect in HCC. Conclusions CircFOXK2, a prognostic biomarker of the disease, exerts critical roles in promoting the Warburg effect through its roles in protein encode and miRNA sponge so that leading tumor progression, indicating that circFOXK2 may serve as a potential therapeutic target for patients with HCC.

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Section: Discussionmentioning

confidence: 99%

CircFOXK2 Promotes Hepatocellular Carcinoma Progression and Leads a Poor Clinical Prognosis via Regulating the Warburg Effect

Zheng

Pan

et al. 2022

Preprint

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Background: The Warburg effect is essential for tumor progression causing poor clinical outcomes in patients with hepatocellular carcinoma (HCC). Circular RNAs (circRNAs) have emerged as important regulators for HCC. However, limited circRNAs involved in the Warburg effect of HCC have been investigated. Herein, we aimed to explore the contribution of circFOXK2 to the reprogramming of glucose metabolism of HCC. Methods: Divergent primers were constructed to identify 14 circRNAs originating from FOXK2 gene followed by investigating their differential expression between HCC and the adjacent normal tissues (ANTs), and circFOXK2 (hsa_circ_0000817) was screened for further research. Next, the clinical significance of circFOXK2 was evaluated, coupled with evaluating its onco-promoting activity and the affection to the Warburg effect in both HCC cell lines and animal xenografts. Finally, the molecular mechanisms underlying circFOXK2 regulating the Warburg effect of HCC were explored. Results: CircFOXK2 was aberrantly upregulation in HCC tissues, and its expression was positively correlated with poor clinical outcomes in patients receiving radical hepatectomy. Silenced circFOXK2 remarkably suppressed the progression of HCC both in vitro and in vivo. Mechanistically, circFOXK2 could not only encode a novel protein, FOXK2-142aa, to promote LDHA phosphorylation but also regulate miR-484/Fis1 pathway to lead to mitochondrial fission, which activates the Warburg effect in HCC. Conclusions: CircFOXK2, a prognostic biomarker of the disease, exerts critical roles in promoting the Warburg effect through its roles in protein-encoding and miRNA sponges that lead to tumor progression, indicating that circFOXK2 may serve as a potential therapeutic target for patients with HCC.

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Retraction Note to: miR-484 suppresses proliferation and epithelial–mesenchymal transition by targeting ZEB1 and SMAD2 in cervical cancer cells

Abstract: This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1186/s12935-021-01958-0

Cited by 3 publications

References 1 publication

Use of miRNA Sequencing to Reveal Hub miRNAs and the Effect of miR-582-3p/SMAD2 in the Progression of Hepatocellular Carcinoma

Use of miRNA Sequencing to Reveal Hub miRNAs and the Effect of miR-582-3p/SMAD2 in the Progression of Hepatocellular Carcinoma

CircFOXK2 Functions as a Protein Encode and a microRNA Sponge to Promote Hepatocellular Carcinoma Progression and Lead a Poor Clinical Prognosis via Regulating the Warburg Effect

CircFOXK2 Promotes Hepatocellular Carcinoma Progression and Leads a Poor Clinical Prognosis via Regulating the Warburg Effect

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