Investigation of a method for long-term preservation of the vascular allograft

Abstract

Background/objective During multiple organ procurement, blood vessels are routinely retrieved and stored in University of Wisconsin solution and then discarded after two weeks, if not used at organ transplantation owing to lack of a method for long-term preservation. Therefore, the aim of this study is to investigate a method for long-term preservation of vascular allografts in ethanol. Methods Aorta and vena cava allografts were retrieved and stored in 75% ethanol solution for 12 months at 4°C. Four pigs were divided into two groups. A segment of aorta was excised and replaced by insertion of preserved aorta graft (Group A) or vena cava graft (Group V). The pigs were observed for six weeks. A laparotomy was performed and the vascular graft was harvested for histopathology followed by euthanasia at the end of study. Results Three pigs recovered uneventfully, while one pig died from venous graft rupture in the third week after surgery. There was no aneurysmal formation or thrombosis in the grafts. Some calcification was seen over aorta allograft. On histopathology, the elastic pattern was almost normal, although the endothelial cells degenerated after preservation. After implantation, the formation of the endothelium cell-like layer was seen in both aorta and vena cava allografts. Conclusion Vascular allografts were functional after preservation for 12 months. The vena cava grafts had much less wall calcification than the aorta grafts. Further studies are necessary to investigate vascular graft remodelling with a longer observation period after implantation.

Remote Outcomes with Poly-ε-Caprolactone Aortic Grafts in Rats

“…There is plenty of research considering synthetic materials for vascular grafting [1,2,[27][28][29][30][31][32]. However, opinions on the importance of complete resemblance of native vessel biomechanics before the graft vitalization were divided.…”

“…Calcification is one of the most frequent complications of vascular grafting. This problem is not specific to artificial scaffolds but is also relevant to xenogeneic and allogeneic materials [26][27][28][29][30][31]. Scientists have been facing implant mineralization in different animal models, with devices made of different materials [31][32][33].…”