Selection of ESBL-producing Escherichia coli in the gut of calves experimentally fed with milk containing antibiotic residues

Dupouy, Véronique; Madec, Jean‐Yves; Wucher, Jessica; Arpaillange, Nathalie; Métayer, Véronique; Roques, Béatrice; Bousquet‐mélou, Alain; Haenni, Marisa

doi:10.1016/j.vetmic.2021.109049

Cited by 7 publications

(8 citation statements)

References 20 publications

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“…Penati et al [46] observed that calves fed with milk containing residual cefalexin (first GC) differed for GIM composition, with a final higher abundance of Chlamydiae phylum compared to an untreated group until 6 weeks after cefalexin residual fed withdrawal. Dupouy et al [47] investigated the selective power of cefquinome (fourth GC) administered to calves colonized by different amounts of Extended-Spectrum-Beta-Lactamase (ESBL) producing E. coli. The administration mimicked residual concentration found in waste milk (2 mg/L) and that of udder milk of treated cows (20 mg/L).…”

Section: Effect Of Waste Milk Feeding On Calves' Gimmentioning

confidence: 99%

Impact of Antibiotic Therapies on Resistance Genes Dynamic and Composition of the Animal Gut Microbiota

Rochegüe

Haenni

Mondot

et al. 2021

Animals

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Antibiotics are major disruptors of the gastrointestinal microbiota, depleting bacterial species beneficial for the host health and favoring the emergence of potential pathogens. Furthermore, the intestine is a reactor of antibiotic resistance emergence, and the presence of antibiotics exacerbates the selection of resistant bacteria that can disseminate in the environment and propagate to further hosts. We reviewed studies analyzing the effect of antibiotics on the intestinal microbiota and antibiotic resistance conducted on animals, focusing on the main food-producing and companion animals. Irrespective of antibiotic classes and animal hosts, therapeutic dosage decreased species diversity and richness favoring the bloom of potential enteropathogens and the selection of antibiotic resistance. These negative effects of antibiotic therapies seem ineluctable but often were mitigated when an antibiotic was administered by parenteral route. Sub-therapeutic dosages caused the augmentation of taxa involved in sugar metabolism, suggesting a link with weight gain. This result should not be interpreted positively, considering that parallel information on antibiotic resistance selection was rarely reported and selection of antibiotic resistance is known to occur also at low antibiotic concentration. However, studies on the effect of antibiotics as growth promoters put the basis for understanding the gut microbiota composition and function in this situation. This knowledge could inspire alternative strategies to antibiotics, such as probiotics, for improving animal performance. This review encompasses the analysis of the main animal hosts and all antibiotic classes, and highlights the future challenges and gaps of knowledge that should be filled. Further studies are necessary for elucidating pharmacodynamics in animals in order to improve therapy duration, antibiotic dosages, and administration routes for mitigating negative effects of antibiotic therapies. Furthermore, this review highlights that studies on aminoglycosides are almost inexistent, and they should be increased, considering that aminoglycosides are the first most commonly used antibiotic family in companion animals. Harmonization of experimental procedures is necessary in this research field. In fact, current studies are based on different experimental set-up varying for antibiotic dosage, regimen, administration, and downstream microbiota analysis. In the future, shotgun metagenomics coupled with long-reads sequencing should become a standard experimental approach enabling to gather comprehensive knowledge on GIM in terms of composition and taxonomic functions, and of ARGs. Decorticating GIM in animals will unveil revolutionary strategies for medication and improvement of animals’ health status, with positive consequences on global health.

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Section: Effect Of Waste Milk Feeding On Calves' Gimmentioning

confidence: 99%

Impact of Antibiotic Therapies on Resistance Genes Dynamic and Composition of the Animal Gut Microbiota

Rochegüe

Haenni

Mondot

et al. 2021

Animals

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“…In the study, calves were fed with milk containing 727 ng/ml of antibiotics, an amount that is higher than the MRL determined for this antibiotic. In the experimental study by Dupouy et al (2021), calves were fed with milk containing residual antibiotic cefquinome with concentrations of 2,000, 20,000 µg/L. This treatment continued for 3 days.…”

Section: Discussionmentioning

confidence: 99%

“…By examining the bacterial feces of calves, it was observed that the number of Escherichia bacteria increased. The observed changes were not dose-dependent (Dupouy et al, 2021). A fecal medium was used in a study to evaluate the residual effect of the ciprofloxacin antibiotic on the intestinal microbiota.…”

Section: Discussionmentioning

confidence: 99%

The effect of residual antibiotics in food on intestinal microbiota: a systematic review

Sadighara

Rostami

Shafaroodi

et al. 2023

Front. Sustain. Food Syst.

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IntroductionThe gut microbiota may be altered following changes in diet or exposure to drugs. Humans can be exposed to antibiotic residue in food. People may be exposed to these compounds for years. But in determining the maximum residue level (MRL), the effects of antibiotic residue on the intestinal microbiota are not investigated. Some evidence suggests that antibiotics in small amounts also lead to changes in the intestinal microbiota. Therefore, a systematic study was conducted with the aim of investigating the effect of antibiotic residues in food on the intestinal microbiota.MethodThe main criterion of this research was to investigate the effects of antibiotics at low doses. For this purpose, a search was made in the databases with keywords antibiotic, veterinary antibiotic, food, residue, microbiome, and microbiota. The investigated doses of each of the antibiotics in the studies were compared with their MRL in food.ResultsThe most significant change in the structure and function of the microbiota was made by tetracycline, sulfamethoxazole, cefquinome, florfenicol and tylosin. The lowest observed effect was related to the antibiotics fosfomycin and amoxicillin.DiscussionExposure to antibiotic residues through food is usually a long-term exposure. In vivo studies, changes in the intestinal microbiota were observed. Therefore, it is necessary to inform the breeders and competent authorities in order to comply with the principles of treatment. The gut microbiota may be altered following changes in diet or exposure to drugs.

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“…Milk from dairy cows under antibiotic treatment for mastitis must be retained, following the recommended withholding period. However, this milk is frequently fed to calves, before the due time, posing a risk for AMR selection on calves’ gut, even at very low residue concentrations 41 , 42 . Additionally, colostrum of freshly calved cows may contain CP or CL residues (used for dry cow therapy), which may affect the newborn calf intestinal flora by imposing a selective pressure for resistant bacteria.…”

Section: Discussionmentioning

confidence: 99%

Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments

Speksnijder

Hopman

Kusters

et al. 2022

Sci Rep

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Selection and spread of Extended Spectrum Beta-Lactamase (ESBL) -producing Enterobacteriaceae within animal production systems and potential spillover to humans is a major concern. Intramammary treatment of dairy cows with first-generation cephalosporins is a common practice and potentially selects for ESBL-producing Enterobacteriaceae, although it is unknown whether this really occurs in the bovine fecal environment. We aimed to study the potential effects of intramammary application of cephapirin (CP) and cefalonium (CL) to select for ESBL-producing Escherichia coli in the intestinal content of treated dairy cows and in manure slurry, using in vitro competition experiments with ESBL and non-ESBL E. coli isolates. No selection of ESBL-producing E. coli was observed at or below concentrations of 0.8 µg/ml and 4.0 µg/ml in bovine feces for CP and CL, respectively, and at or below 8.0 µg/ml and 4.0 µg/ml, respectively, in manure slurry. We calculated that the maximum concentration of CP and CL after intramammary treatment with commercial products will not exceed 0.29 µg/ml in feces and 0.03 µg/ml in manure slurry. Therefore, the results of this study did not find evidence supporting the selection of ESBL-producing E. coli in bovine feces or in manure slurry after intramammary use of commercial CP or CL-containing products.

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Selection of ESBL-producing Escherichia coli in the gut of calves experimentally fed with milk containing antibiotic residues

Cited by 7 publications

References 20 publications

Impact of Antibiotic Therapies on Resistance Genes Dynamic and Composition of the Animal Gut Microbiota

Impact of Antibiotic Therapies on Resistance Genes Dynamic and Composition of the Animal Gut Microbiota

The effect of residual antibiotics in food on intestinal microbiota: a systematic review

Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments

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