Rational design of a HA-AuNPs@AIED nanoassembly for activatable fluorescence detection of HAase and imaging in tumor cells

Abstract

Aggregation induced emission (AIE) dots have gained broad attention in fluorescence bioimaging and biosensor in virtue of their distinctive optical properties of splendid biocompatibility, high brightness and good photostability. However,...

Biosensors for the Detection of Enzymes Based on Aggregation-Induced Emission

“…The enzymatic digestion of HA was limited the assembly of TPE-4N + , causing the fluorescence quenching. Moreover, based on the quenching ability of AuNPs, Wang et al reported the detection of HAase with AIE-dot-based nanoprobes (AIEDs) [ 115 ]. In this method, the negatively charged HA stabilized AuNPs and promoted their assembly on AIEDs to form HA-AuNPs@AIEDs, thus quenching the fluorescence through fluorescence resonance energy transfer.…”

An AIEgen-based “turn-on” probe for sensing cancer cells and tiny tumors with high furin expression

“…25 Based on the FRET principle, introducing a quencher to fluorophores is a common way to suppress the background noise, 26 Except that, positively charged AIE probes have the risk of inducing hemolysis. 27 Hyaluronic acid, 28 PEG 29 and γ-PGA 30 have been reported to improve the biocompatibility of positively charged probes.…”

Zinc (II) and AIEgens: The “Clip Approach” for a Novel Fluorophore Family. A Review

“…In 2005, Tang and coworkers produced chemosensors based on silole as sensors for the regioisomers of nitroanilines [ 57 ] and in 2010, Park and coworkers promoted AIEgens as stimuli–responsive materials. To date, several AIE-based chemosensors were reported for the detection of ions, metals, small organic molecules, and biological targets [ 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 ].…”

“…Porous crystalline solids such as inorganic nanoparticles (NPs), metal–organic frameworks (MOFs), covalent organic frameworks (COFs), and carbon nanotubes (CNTs) are materials where the choice of suitable AIE active fragments can produce highly engineered composite materials with unique properties [ 59 , 60 , 61 ]. In the composite material, the AIE moieties interact with a regular structure resulting in tunable electronic and optical properties.…”

“…Low self-quenching and a highly modulable inner chemical environment can be achieved. Thanks to the porous architecture, such nanostructured AIEgens are promising candidates for fluorescence sensing with high sensitivity and selectivity toward specific analytes [ 61 , 62 , 63 , 64 , 65 , 66 ].…”