Synbiotic sheep milk ice cream reduces chemically induced mouse colon carcinogenesis

Abstract

Sheep dairy products containing prebiotic and probiotic ingredients may have health-promoting properties. Thus, this study evaluated the effects of sheep milk ice cream [conventional full-fat (CONV), full-fat enriched with probiotic (PROB, 100 mg % wt/wt of Lacticaseibacillus casei 01), or nonfat synbiotic (SYNB, Lacticaseibacillus casei 01 and inulin, 10% wt/wt)] on carcinogen-induced colonic crypt cytotoxicity and premalignant lesion development. Male Swiss mice received 2 doses of colon carcinogen azoxymethane (AOM, 15 mg/kg of body weight) at wk 3 and 4. Two weeks before and during AOM administrations (4 wk) mice were treated with CONV, PROB, or SYNB by gavage (10 mL/kg). Mice were euthanized at wk 4 or 25 (n = 5 or 10 mice/group, respectively). At wk 4, a significant reduction in micronucleated colonocytes was observed in PROB and SYNB groups, and a significant decrease in both p53 expression and apoptosis indexes in colonic crypts was observed in SYNB group. At wk 25, both PROB and SYNB interventions reduced the mean number of colonic premalignant lesions. However, only SYNB group showed lower incidence and number of high-grade premalignant lesions in the colonic mucosa. These findings indicate that PROB or SYNB sheep milk ice cream, especially SYNB intervention, can reduce chemically induced mouse colon carcinogenesis.

Mesenchymal stem cell conditioned medium azacytidine, panobinostat and GSK126 alleviate TGF-β-induced EMT in lung cancer

“…In addition, panobinostat increases nuclear histone and non-histone acetylation, thus regulating gene expression and protein activity associated with cancer cell growth and survival pathway (Crisanti et al, 2009). GSK126, as an EZH2 selective inhibitor, is closely related to the occurrence and development of tumors (Zeng et al, 2017;Balthazar et al, 2021). Our results showed that azacytidine, panobinostat and GSK126 respectively inhibited cell viability and invasion in a dose-dependent manner.…”

CLDN18-ARHGAP26 function in gastric cancer and be a new therapeutic target by ABCG2 and ABCB1 pathway

“…The majority of family members harbor various biological functions, including promoting cell proliferation and differentiation, inhibiting cell apoptosis and intercellular interactions, which exert regulatory effects on critical biological and pathological process (Hashimoto et al, 2019;Ismail et al, 2020;Li et al, 2020;Negrão et al, 2021). The migration, proliferation and differentiation of lumen morphology would lead to the angiogenesis of tumor (Balthazar et al, 2021;Hu et al, 2021;Khan et al, 2021). Recent studies have demonstrated that CLDN18, a member of CLDN family, is involved in regulation of angiogenesis of tumor, playing a vital role in the tumorigenesis and metastasis (Coati et al, 2019).…”

Probiotic lactobacilli in faeces of breastfed babies

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