Involvement of glycolysis activation in flatfish sexual size dimorphism: Insights from transcriptomic analyses of Platichthys stellatus and Cynoglossus semilaevis

Wang, Na; Tian, Yongsheng; Zhang, Jingjing; Li, Zhentong; Cheng, Mei‐Ling; Wu, Yuping

doi:10.1016/j.cbd.2021.100832

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…The activation of glycolysis/gluconeogenesis in female muscle tissues contributes to the sexual size in flatfish. Several important genes involved in glycolysis (e.g., gpi, aldo, tpi, gapdh, bpgm, pgk, eno2, pk, and ldh) were expressed more by several degrees in the female muscle tissues than in the male muscle tissues [27]. Furthermore, the enzyme pyruvate kinase M2 (PKM2) of glycolysis is an important metabolic means of mediating the effects of growth signals to promote cell proliferation; growth stimulations also lead to PKM2 phosphorylation which regulates the conversion of protein kinase and pyruvate kinase activities [28].…”

Section: Differences In the Metabolism Of F1 Hybrid Gymnocypris And I...mentioning

confidence: 97%

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Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Growth Superiority in a Novel Gymnocypris Hybrid, Gymnocypris przewalskii ♀ × Gymnocypris eckloni ♂

Zhao,

Zhou,

Dong

et al. 2024

Genes

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Artificial hybrid breeding can optimize parental traits to cultivate excellent hybrids with enhanced economic value. In this study, we investigated the growth performance and transcriptomes of Gymnocypris przewalskii (♀) and Gymnocypris eckloni (♂) and their F1 hybrid fishes. Hatched individuals of G. przewalskii (GP) and G. eckloni (GE) of the same size and their F1 hybrids (GH) were separately cultured for eight months in three cement tanks (n = 3). The growth indexes were measured, which showed that the growth rate of the groups was GE > GH > GP, while the survival rate was GH > GE > GP. The RNA-Seq data analysis of the muscles from the three Gymnocypris fish strains revealed that gene transcription has a significant impact on F1 hybrid fish and its parents. The differentially expressed genes (DEGs) in GH show less differences with GP, but more with GE. qRT-PCR was used to confirm the expression profiles of the chosen DEGs, and the results showed positive correlations with the RNA-seq data. KEGG enrichment results indicated that the DEGs were related to a variety of molecular functions, such as glycolysis/gluconeogenesis, arachidonic acid formation, citrate cycle, and the MAPK, PI3K-Akt, or mTOR signal pathways. Subsequent analysis indicated that there may be a significant correlation between the differential expression of IGF2 and a difference in the growth of GE and GP.

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Section: Differences In the Metabolism Of F1 Hybrid Gymnocypris And I...mentioning

confidence: 97%

“…Previous studies have revealed the role of the glycolysis/gluconeogenesis pathway (ko00010) in promoting muscle growth, which largely involves glycolytic genes [26,27]. The activation of glycolysis/gluconeogenesis in female muscle tissues contributes to the sexual size in flatfish.…”

Section: Differences In the Metabolism Of F1 Hybrid Gymnocypris And I...mentioning

confidence: 99%

Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Growth Superiority in a Novel Gymnocypris Hybrid, Gymnocypris przewalskii ♀ × Gymnocypris eckloni ♂

Zhao,

Zhou,

Dong

et al. 2024

Genes

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Section: Differences In the Metabolism Pathwaysmentioning

confidence: 99%

“…The glycolysis/gluconeogenesis pathway, primarily regulated by glycolytic genes, has been demonstrated in earlier research to contribute to muscle growth stimulation [30,31]. Studies indicate that the activation of glycolysis/gluconeogenesis in female muscle tissues contributes to sexual size dimorphism in flatfish as key genes involved in glycolysis (e.g., gpi, aldo, tpi, gapdh, bpgm, pgk, eno2, pk and ldh) were expressed to a greater extent in female muscle tissues than in male muscle tissues [31]. In our study, most genes related to glycolysis/gluconeogenesis were downregulated in G. przewalskii, including ENO3 (beta-enolase), PKM (pyruvate kinase), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), PGM-1 (phosphoglucomutase-1), and FBP2 (fructose-1,6-bisphosphatase isozyme 2).…”

Section: Differences In the Metabolism Pathwaysmentioning

confidence: 99%

Molecular Mechanisms of Growth Differences in Gymnocypris przewalskii and Gymnocypris eckloni through a Comparative Transcriptome Perspective

Zhao,

Zhou

2024

Fishes

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Genetic composition plays a crucial role in the growth rate of species, and transcriptomics provides a potent tool for studying genetic aspects of growth. We explored the growth rates and transcriptomes of the Cyprinids G. przewalskii (GP) and G. eckloni (GE). A total of 500 individuals of G. przewalskii and G. eckloni, matched in size, were separately cultured for 9 months in six cement tanks (each group with three replicates). Growth indices were measured, revealing that the growth rate of GE was greater than that of GP (p < 0.05), while there was no significant difference in survival rates (p > 0.05). Simultaneously, we conducted RNA-Seq on the muscles of both GP and GE. The results indicated a significant difference of gene expression between GP and GE, identifying 5574 differentially expressed genes (DEGs). Quantitative real-time reverse transcription–polymerase chain reaction of 10 DEGs demonstrated consistency in expression profiles with the results from the RNA-Seq analysis. The DEGs were significantly enriched in glycolysis/gluconeogenesis (ko00010), arachidonic acid formation (ko00061), arginine biosynthesis (ko00220), and the MAPK (ko04013), PI3K-Akt (ko04151), mTOR (ko04150), and TGF-β (ko04350) signal pathways, as revealed by Gene Ontology (GO) enrichment and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. This study also identified some growth-related DEGs, such as IGF2, Noggin, Decorin and others. Notably, the low expression of IGF2 may be a factor contributing to the slower growth of GP than GE.

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Decoding the fish genome opens a new era in important trait research and molecular breeding in China

Zhou,

Wang,

et al. 2024

Sci. China Life Sci.

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Involvement of glycolysis activation in flatfish sexual size dimorphism: Insights from transcriptomic analyses of Platichthys stellatus and Cynoglossus semilaevis

Cited by 6 publications

References 29 publications

Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Growth Superiority in a Novel Gymnocypris Hybrid, Gymnocypris przewalskii ♀ × Gymnocypris eckloni ♂

Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Growth Superiority in a Novel Gymnocypris Hybrid, Gymnocypris przewalskii ♀ × Gymnocypris eckloni ♂

Molecular Mechanisms of Growth Differences in Gymnocypris przewalskii and Gymnocypris eckloni through a Comparative Transcriptome Perspective

Decoding the fish genome opens a new era in important trait research and molecular breeding in China

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