Molecular Imaging of Apoptosis: The Case of Caspase-3 Radiotracers

Beroske, Lucas; Wyngaert, Tim Van den; Stroobants, Sigrid; Veken, Pieter Van der; Elvas, Filipe

doi:10.3390/ijms22083948

Cited by 60 publications

(38 citation statements)

References 73 publications

(62 reference statements)

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“…However, the activation of the cysteine-oriented protease family, which activates caspase-3, is also considered a specific apoptotic marker (Beroske et al, 2021). In addition, the B-cell lymphoma 2 (Bcl-2) family of proteins promotes apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Artemisia capillaris Thunb. water extract attenuates adriamycin‐induced renal injury by regulating apoptosis through the ROS/MAPK axis

Huang

Zhu

Chen

et al. 2022

Journal of Food Biochemistry

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Artemisia capillaris Thunb. is widely used in the treatment of kidney diseases, but the underlying mechanism remain elusive. Therefore, this study aimed to elucidate the mechanism of Artemisia capillaris Thunb. in alleviating renal injury. And renoprotective effects of freeze-dried powder of Artemisia capillaris Thunb. water extract (WAC) were assessed using adriamycin (ADR)-induced renal injury to the NRK-52E cells and ADR-induced renal injury Sprague-Dawley rats (SD rats) models. The results show that WAC could alleviate ADR-induced renal injury in SD rats and the NRK-52E cell line,

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Section: Discussionmentioning

confidence: 99%

Artemisia capillaris Thunb. water extract attenuates adriamycin‐induced renal injury by regulating apoptosis through the ROS/MAPK axis

Huang

Zhu

Chen

et al. 2022

Journal of Food Biochemistry

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“…Therefore, apoptosis was first selected to be discussed in this study. In terms of HaCaT cells, high doses of MCLR, GO and GO-MCLR, significantly activated apoptosis-related protein caspase-3/7 in HaCaT cells [ 39 ], but flow cytometry results did not show strong apoptosis except for high dose of GO. The possible reason was that due to its indigestion characteristics, the long digestion time of HaCaT cells during the flow cytometry assay of apoptosis affected the effective binding of fluorescent probes.…”

Section: Discussionmentioning

confidence: 99%

Safety assessment of graphene oxide and microcystin-LR complex: a toxicological scenario beyond physical mixture

Ding

Liu

et al. 2022

Part Fibre Toxicol

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Background Nanomaterials have been widely used in electrochemistry, sensors, medicine among others applications, causing its inevitable environmental exposure. A raising question is the “carrier” effect due to unique surface properties of nanomaterials, which may collectively impact the bioavailability, toxicokinetic, distribution and biological effects of classic toxicants. Noteworthy, this aspect of information remains largely unexplored. Methods Here, we deliberately selected two entities to mimic this scenario. One is graphene oxide (GO), which is made in ton quantity with huge surface-area that provides hydrophilicity and π–π interaction to certain chemicals of unique structures. The other is Microcystin-LR (MCLR), a representative double-bond rich liver-toxic endotoxin widely distributed in aquatic-system. Firstly, the adsorption of GO and MCLR after meeting under environmental conditions was explored, and then we focused on the toxicological effect and related mechanism of GO-MCLR complex on human skin cutin forming cells (HaCaT cells) and normal liver cells (L02 cells). Results Abiotically, our study demonstrated that GO could effectively adsorb MCLR through hydrogen bonding and π–π interaction, the oxidation degree of GO-MCLR decreased significantly and surface defect level raised. Compared to GO or MCLR, GO-MCLR was found to induce more remarkable apoptosis and ferroptosis in both HaCaT and L02 cells. The underlying mechanism was that GO-MCLR induced stronger intracellular reactive oxygen species (ROS) and mtROS generation, followed by Fe2+ accumulation, mitochondrial dysfunction and cytoskeletal damage. Conclusions These results suggest that the GO-MCLR complex formed by GO adsorption of MCLR may exhibit more toxic effects than the single material, which demonstrates the necessity for assessing nano-toxicant complexity. Our discovery may serve as a new toxicological paradigm in which nanomaterial mediated surface adsorption effects could impact the degree of cytotoxicity and toxicological mechanisms of classic toxins. Graphical Abstract

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“…For instance, imaging agents [ 18 F]ICMT-11 or Annexin V were tested clinically, targeting activated caspase-3 or externalized phosphoserines, respectively. However, these tracers suffered from low specificity and high abdominal uptake [ 1 , 36 ]. Therefore, many efforts are made to generate better contrast agents for imaging cell death.…”

Section: Discussionmentioning

confidence: 99%

Improved Multimodal Tumor Necrosis Imaging with IRDye800CW-DOTA Conjugated to an Albumin-Binding Domain

Stroet

Blois

Jong

et al. 2022

Cancers

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Purpose: To assess our improved NACA for the detection of tumor necrosis. Methods: We increased the blood circulation time of our NACA by adding an albumin-binding domain to the molecular structure. We tested the necrosis avidity on dead or alive cultured cells and performed SPECT and fluorescence imaging of both spontaneous and treatment-induced necrosis in murine breast cancer models. We simultaneously recorded [18F]FDG-PET and bioluminescence images for complementary detection of tumor viability. Results: We generated two albumin-binding IRDye800CW derivatives which were labeled with indium-111 with high radiochemical purity. Surprisingly, both albumin-binding NACAs had >10x higher in vitro binding towards dead cells. We selected [111In]3 for in vivo experiments which showed higher dead cell binding in vitro and in vivo stability. The doxorubicin-treated tumors showed increased [111In]3-uptake (1.74 ± 0.08%ID/g after saline treatment, 2.25 ± 0.16%ID/g after doxorubicin treatment, p = 0.044) and decreased [18F]FDG-uptake (3.02 ± 0.51%ID/g after saline treatment, 1.79 ± 0.11%ID/g after doxorubicin treatment, p = 0.040), indicating therapy efficacy. Moreover, we detected increased [111In]3-uptake and tumor necrosis in more rapidly growing EMT6 tumors. Conclusions: Our albumin-binding NACA based on IRDye800CW facilitates tumor-necrosis imaging for assessment of therapy efficacy and aggressiveness in solid tumors using both fluorescence and SPECT imaging.

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Molecular Imaging of Apoptosis: The Case of Caspase-3 Radiotracers

Cited by 60 publications

References 73 publications

Artemisia capillaris Thunb. water extract attenuates adriamycin‐induced renal injury by regulating apoptosis through the ROS/MAPK axis

Artemisia capillaris Thunb. water extract attenuates adriamycin‐induced renal injury by regulating apoptosis through the ROS/MAPK axis

Safety assessment of graphene oxide and microcystin-LR complex: a toxicological scenario beyond physical mixture

Improved Multimodal Tumor Necrosis Imaging with IRDye800CW-DOTA Conjugated to an Albumin-Binding Domain

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