Campylobacter vulpis sp. nov. isolated from wild red foxes

Parisi, Antonio; Chiara, Matteo; Caffara, Monica; Mion, Domenico; Miller, William G.; Caruso, Marta; Manzari, Caterina; Florio, Daniela; Capozzi, Loredana; D’Erchia, Anna Maria; Manzulli, Viviana; Zanoni, Renato Giulio

doi:10.1016/j.syapm.2021.126204

“…We note that Campylobacter ureolyticus DSMZ 20703 was previously reported to encode homologues of T6SS components [ 40 ], but it was not present in the assembly data downloaded from NCBI and thus not investigated. We also note that several recently identified novel species of the genus Campylobacter were also absent from the NCBI RefSeq release 205 database, including Campylobacter aviculae, Campylobacter estrildidarum, Campylobacter taeniopygiae [ 70 ] , Campylobacter portucalensis [ 71 ] , Campylobacter novaezeelandiae [ 72 ] , Campylobacter massiliensis [ 73 ] and Campylobacter vulpis [ 74 ], of which two were discovered after the database was downloaded. As such, our database does not encompass all Campylobacter species to date, and therefore we stress that the results of our study are exclusive to species only included in the local database.…”

Section: Discussionmentioning

confidence: 99%

In silico investigation of the genus Campylobacter type VI secretion system reveals genetic diversity in organization and putative effectors

Robinson

¹

,

Liaw

²

,

Omole

³

et al. 2022

View full text Add to dashboard Cite

Bacterial type VI secretion systems (T6SSs) are contractile nanomachines that deliver proteinic substrates into target prokaryotic or eukaryotic cells and the surrounding milieu. The genus Campylobacter encompasses 39 recognized species and 13 subspecies, with many belonging to a group known as ‘emerging Campylobacter pathogens’. Within Campylobacter , seven species have been identified to harbour a complete T6SS cluster but have yet to be comparatively assessed. In this study, using systematic bioinformatics approaches and the T6SS-positive Campylobacter jejuni 488 strain as a reference, we explored the genus-wide prevalence, similarity and make-up of the T6SS amongst 372 publicly available ‘complete’ Campylobacter genomes. Our analyses predict that approximately one-third of Campylobacter species possess a T6SS. We also putatively report the first identification of a T6SS in four species: Campylobacter cuniculorum, Campylobacter helveticus, Campylobacter armoricus and Campylobacter ornithocola . The Campylobacter T6SSs cluster into three distinct organizations (I–III), of which two break down into further variants. Thirty T6SS-containing genomes were found to harbour more than one vgrG gene, with Campylobacter lari strain NCTC 11845 possessing five. Analysis of the C. jejuni Pathogenicity Island-1 confirmed its conservation amongst T6SS-positive C. jejuni strains, as well as highlighting its diverse genetic composition, including additional putative effector–immunity pairs (e.g. PoNe and DUF1911 domains). Effector–immunity pairs were also observed neighbouring vgrGs in several other Campylobacter species, in addition to putative genes encoding nucleases, lysozymes, ATPases and a ferric ATP-binding cassette uptake system. These observations highlight the diverse genetic make-up of the T6SS within Campylobacter and provide further evidence of its role in pathogenesis.

show abstract

“…However, the other emerging Campylobacter pathogens are gaining increasing recognition as important pathogens in humans and animals [6]. In addition, with the continuous progress of isolation and culture technology, more and more novel Campylobacter species have been identified in recent years [7][8][9][10][11][12][13][14]. Domestic animals, especially pets, have close contact with humans.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Genomic Characteristics of Cat-Borne Campylobacter felis sp. nov. and Sheep-Borne Campylobacter ovis sp. nov

Wang

¹

,

Li

²

,

Gu

³

et al. 2023

View full text Add to dashboard Cite

Nine novel bacterial strains were isolated from the feces of cats and sheep in 2019 and 2020 in Beijing, China. Cells were 1–3 μm long and ≤0.5 μm wide, Gram-stain negative, microaerobic, motile, oxidase positive, and urease negative. Phylogenetic analyses based on 16S rRNA gene sequences indicated that these nine isolates belong to the genus Campylobacter but formed two robust clades that were clearly separate from the currently recognized species and, respectively, isolated from the cat and sheep. Both these strains shared low 16S rRNA gene sequence similarity, dDDH relatedness, and ANI values with their closest species C. upsaliensis CCUG 14913T and C. lanienae NCTC 13004T, and against each other, which are below the cut-off values generally recognized for isolates of the same species. The genomic DNA G + C contents of type strains XJK22-1T and SYS25-1T were 34.99 mol% and 32.43 mol%, respectively. Electron microscopy showed that these cells were spiral shaped, with bipolar single flagella. Based on results from genotypic, phenotypic, phylogenetic, and phylogenomic analyses, these nine strains represent two novel species within the genus Campylobacter, for which the names Campylobacter felis sp. nov. (Type strain XJK22-1T = GDMCC 1.3684T = JCM 35847T) and Campylobacter ovis sp. nov. (Type strain SYS25-1T = GDMCC 1.3685T) are proposed.

show abstract

“…The type strain of this novel species also stands out due to its inability to hydrolyze hippurate, while it exhibits the capability to hydrolyze indoxyl acetate and reduce nitrate, setting it apart from C. suis . Finally, PS10 T could be unequivocally differentiated from C. mucosalis due to its lack of oxidase activity and inability to generate H 2 S ( Table 1 ) [ 57 , 58 , 59 , 60 ]. In the realm of biochemical phenotype, isolate PS10 T showcases distinctive attributes within the Campylobacter genus, allowing for its precise differentiation from other Campylobacter species through its unique biochemical profiles.…”

Section: Resultsmentioning

confidence: 99%

Phenotypic and Genomic Characteristics of Campylobacter gastrosuis sp. nov. Isolated from the Stomachs of Pigs in Beijing

Wang,

Gu,

He

et al. 2023

Microorganisms

0

View full text Add to dashboard Cite

Campylobacter is among the four main causes of gastroenteritis worldwide. Most reported Campylobacter infections are caused by C. jejuni and C. coli. However, other emerging Campylobacter pathogens have been recognized as important pathogens in humans and animals. A novel bacterial strain, PS10T, was isolated from the gastric mucous of pigs in 2022 in Beijing, China. The cell was Gram-negative, microaerobic, motile, and negative for catalase, oxidase, and urease. Phylogenetic and phylogenomic analyses based on the 16S rRNA gene and core genome indicated that this isolate belongs to the genus Campylobacter. There were low dDDH relatedness and ANI values shared within this strain and its closest species C. mucosalis below the cut-off values generally recognized for isolates of the same species. The draft genome size of PS10T is 2,240,910 bp in length with a percentage of DNA G+C contents of 37.72%. Comparing the phenotypic and phylogenetic features among this isolate and its related organisms, strain PS10T represents a novel species within the genus Campylobacter, for which the name Campylobacter gastrosuis sp. nov. (Type strain PS10T = GDMCC 1.3686T = JCM 35849T) is proposed.

show abstract

Campylobacter vulpis sp. nov. isolated from wild red foxes

Cited by 13 publications

References 45 publications

In silico investigation of the genus Campylobacter type VI secretion system reveals genetic diversity in organization and putative effectors

In silico investigation of the genus Campylobacter type VI secretion system reveals genetic diversity in organization and putative effectors

Isolation and Genomic Characteristics of Cat-Borne Campylobacter felis sp. nov. and Sheep-Borne Campylobacter ovis sp. nov

Phenotypic and Genomic Characteristics of Campylobacter gastrosuis sp. nov. Isolated from the Stomachs of Pigs in Beijing

Contact Info

Product

Resources

About