Construction and immune efficacy of recombinant Lactobacillus casei expressing OmpAI of Aeromonas veronii C5–I as molecular adjuvant

Lin-Zhao, Ze; Tong-Yang, Bin; Yi-Xuan, Yang; Ning-Guo, Song; Xing-Zhang, Dong; Nan-Ji, Sheng; Li, Bing; Huan-Kang, Yuan; Feng-Shan, Xiao; Mei-Shi, Qiu; Wen-Sun, Wu; Dong-Qian, Ai

doi:10.1016/j.micpath.2021.104827

Cited by 11 publications

(11 citation statements)

References 54 publications

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“…The accessibility of recombination Lactobacillus casei to the intestinal tracts in the immunized common carps was examined. Plate counts and colony-direct PCRs showed that approximately equal amounts of Lc-pPG1-Aha1 and Lc-pPG2-Aha1 survived in intestine of fish in both experimental groups, suggesting an survivability of the recombinant L. casei , which was similar to our previous study [ 32 ].…”

Section: Discussionsupporting

confidence: 89%

“…All intestines were harvested and emulsified with PBS, and then serial-diluted (10 3 , 10 4 , 10 5 , 10 6 -fold). To determine the survival of recombination Lactobacillus casei in the carp’s intestines [ 32 , 33 ], the diluted mixtures were plated. Onto MRS agar plates supplemented with Cm, PCRs, and sanger sequencing were performed for verification of selected single colonies.…”

Section: Methodsmentioning

confidence: 99%

“…Each gene expression level was standardized with the expression of β-actin via the 2 −ΔΔCt approach [ 32 ]. The specificity of reactions was verified by analyzing melting curves.…”

Section: Methodsmentioning

confidence: 99%

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Oral vaccination with recombinant Lactobacillus casei expressing Aeromonas hydrophila Aha1 against A. hydrophila infections in common carps

et al. 2022

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The immunogenicity of Aha1, an OMP of Aeromonas hydrophila mediating the adhesion of bacteria onto the mucosal surface of hosts has been established. In this study, recombinant vectors, pPG1 and pPG2, carrying a 1366 bp DNA fragment that was responsible for encoding the 49 kDa Aha1 from A. hydrophila were constructed, respectively, then electroporated into a probiotic strain Lactobacillus casei CC16 separately to generate two types of recombinants, L. casei- pPG1-Aha1 (Lc-pPG1-Aha1) and L. casei -pPG2-Aha1 (Lc-pPG2-Aha1). Subsequently, these were orally administered into common carps to examine their immunogenicity. The expression and localization of the expressed Aha1 protein relative to the carrier L. casei was validated via Western blotting, flow cytometry, and immune fluorescence separately. The recombinant vaccines produced were shown high efficacies, stimulated higher level of antibodies and AKP, ACP, SOD, LZM, C3, C4 in serum in hosts. Immune-related gene expressions of cytokines including IL-10, IL-1β, TNF-α, IFN-γ in the livers, spleens, HK, and intestines were up-regulated significantly. Besides, a more potent phagocytosis response was observed in immunized fish, and higher survival rates were presented in common carps immunized with Lc-pPG1-Aha1 (60%) and Lc-pPG2-Aha1 (50%) after re-infection with virulent strain A. hydrophila . Moreover, the recombinant L. casei were shown a stronger propensity for survivability in the intestine in immunized fish. Taken together, the recombinant L. casei strains might be promising candidates for oral vaccination against A. hydrophila infections in common carps.

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Section: Discussionsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

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Oral vaccination with recombinant Lactobacillus casei expressing Aeromonas hydrophila Aha1 against A. hydrophila infections in common carps

et al. 2022

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“…There, the immune cells could stimulate other cells either by endogenous release of interferon or by the release of interferon produced by MT006, in a mechanism similar to that observed when dendritic cells interact with commensal bacteria in the mammalian intestine and transport them to the lymph nodes (89). This mechanism could explain the immunizing properties of orally administered recombinant Lactic Acid Bacteria that express epitopes from microbial pathogens of fish (33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44). The dosages used in these experiments are between 10 7 to 2x10 8 CFU per fish gram, around 30 to 600 times the used dosage of MT006.…”

Section: Discussionmentioning

confidence: 91%

Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro

et al. 2021

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In salmon farming, viruses are responsible for outbreaks that produce significant economic losses for which there is a lack of control tools other than vaccines. Type I interferon has been successfully used for treating some chronic viral infections in humans. However, its application in salmonids depends on the proper design of a vehicle that allows its massive administration, ideally orally. In mammals, administration of recombinant probiotics capable of expressing cytokines has shown local and systemic therapeutic effects. In this work, we evaluate the use of Lactococcus lactis as a type I Interferon expression system in Atlantic salmon, and we analyze its ability to stimulate the antiviral immune response against IPNV, in vivo and in vitro. The interferon expressed in L. lactis, even though it was located mainly in the bacterial cytoplasm, was functional, stimulating Mx and PKR expression in CHSE-214 cells, and reducing the IPNV viral load in SHK-1 cells. In vivo, the oral administration of this L. lactis producer of Interferon I increases Mx and PKR expression, mainly in the spleen, and to a lesser extent, in the head kidney. The oral administration of this strain also reduces the IPNV viral load in Atlantic salmon specimens challenged with this pathogen. Our results show that oral administration of L. lactis producing Interferon I induces systemic effects in Atlantic salmon, allowing to stimulate the antiviral immune response. This probiotic could have effects against a wide variety of viruses that infect Atlantic salmon and also be effective in other salmonids due to the high identity among their type I interferons.

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“…Immunization of crucian carp ( Carassius carassius ) with recombinant Lactobacillus casei that expresses a fusion protein encoded by of OmpAI gene from Aeromonas veronii and chemokine c5 – i gene (served as a molecular adjuvant) from common carp resulted in enhanced alkaline phosphatase, superoxide dismutase and acid phosphatase in the serum and higher expression of il-10 , il-1β , tnf-α , and ifn-γ in the heart, liver, spleen, head kidney, and intestinal tract. More importantly, recombinant Lactobacillus casei provided strong protection (survival rate at least 60% versus 0% for the unimmunized control group) against A. veronii infection ( 157 ).…”

Section: Orally Administered Immunoprophylactics and The Implication In The Gut Immune Systemmentioning

confidence: 99%

Gut Immune System and the Implications of Oral-Administered Immunoprophylaxis in Finfish Aquaculture

et al. 2021

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The gastrointestinal immune system plays an important role in immune homeostasis regulation. It regulates the symbiotic host-microbiome interactions by training and developing the host’s innate and adaptive immunity. This interaction plays a vital role in host defence mechanisms and at the same time, balancing the endogenous perturbations of the host immune homeostasis. The fish gastrointestinal immune system is armed with intricate diffused gut-associated lymphoid tissues (GALTs) that establish tolerance toward the enormous commensal gut microbiome while preserving immune responses against the intrusion of enteric pathogens. A comprehensive understanding of the intestinal immune system is a prerequisite for developing an oral vaccine and immunostimulants in aquaculture, particularly in cultured fish species. In this review, we outline the remarkable features of gut immunity and the essential components of gut-associated lymphoid tissue. The mechanistic principles underlying the antigen absorption and uptake through the intestinal epithelial, and the subsequent immune activation through a series of molecular events are reviewed. The emphasis is on the significance of gut immunity in oral administration of immunoprophylactics, and the different potential adjuvants that circumvent intestinal immune tolerance. Comprehension of the intestinal immune system is pivotal for developing effective fish vaccines that can be delivered orally, which is less labour-intensive and could improve fish health and facilitate disease management in the aquaculture industry.

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Construction and immune efficacy of recombinant Lactobacillus casei expressing OmpAI of Aeromonas veronii C5–I as molecular adjuvant

Cited by 11 publications

References 54 publications

Oral vaccination with recombinant Lactobacillus casei expressing Aeromonas hydrophila Aha1 against A. hydrophila infections in common carps

Oral vaccination with recombinant Lactobacillus casei expressing Aeromonas hydrophila Aha1 against A. hydrophila infections in common carps

Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro

Gut Immune System and the Implications of Oral-Administered Immunoprophylaxis in Finfish Aquaculture

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