Overview of the Microbiome Among Nurses study (Micro-N) as an example of prospective characterization of the microbiome within cohort studies

Everett, Christine; Li, Chengchen; Wilkinson, Jeremy E.; Nguyen, Long H.; McIver, Lauren J.; Ivey, Kerry L.; Izard, Jacques; Palacios, Natalia; Eliassen, A. Heather; Willett, Walter C.; Ascherio, Alberto; Sun, Qi; Tworoger, Shelley S.; Chan, Andrew T.; Garrett, Wendy S.; Huttenhower, Curtis; Rimm, Eric B.; Song, Mingyang

doi:10.1038/s41596-021-00519-z

Cited by 8 publications

(5 citation statements)

References 39 publications

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“…A total of 837 NHS and 880 HPFS participants were selected and invited into the study. The stool collection protocol in our study followed that of the Micro‐N study, designed and implemented by the Harvard Chan Microbiome Collection Core (HCMCC), as previously described 46 . Briefly, an invitation letter was mailed to eligible NHS and HPFS participants identified as PPS, PD, constipated controls, and healthy controls.…”

Section: Methodsmentioning

confidence: 99%

“…At the time of stool collection, participants completed a 2‐page supplemental stool questionnaire that included questions regarding recent diet, major lifestyle factors, medication (including laxative and stool softener use), and medical history, as well as stool consistency and bowel movement pattern. The supplemental questionnaire used in this study was developed for the Micro‐N study and has been described in detail and published previously 46 …”

Section: Methodsmentioning

confidence: 99%

“…The stool collection protocol in our study followed that of the Micro-N study, designed and implemented by the Harvard Chan Microbiome Collection Core (HCMCC), as previously described. 46 Briefly, an invitation letter was mailed to eligible NHS and HPFS participants identified as PPS, PD, constipated controls, and healthy controls. Participants were asked to review and return a consent form for the stool collection and study.…”

Section: Healthy Controls and Controls With Constipationmentioning

confidence: 99%

“…The supplemental questionnaire used in this study was developed for the Micro-N study and has been described in detail and published previously. 46…”

Section: Assessment Of Covariatesmentioning

confidence: 99%

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Metagenomics of the Gut Microbiome in Parkinson's Disease: Prodromal Changes

Palacios

Wilkinson²,

Bjørnevik

et al. 2023

Annals of Neurology

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ObjectivePrior studies on the gut microbiome in Parkinson's disease (PD) have yielded conflicting results, and few studies have focused on prodromal (premotor) PD or used shotgun metagenomic profiling to assess microbial functional potential. We conducted a nested case–control study within 2 large epidemiological cohorts to examine the role of the gut microbiome in PD.MethodsWe profiled the fecal metagenomes of 420 participants in the Nurses' Health Study and the Health Professionals Follow‐up Study with recent onset PD (N = 75), with features of prodromal PD (N = 101), controls with constipation (N = 113), and healthy controls (N = 131) to identify microbial taxonomic and functional features associated with PD and features suggestive of prodromal PD. Omnibus and feature‐wise analyses identified bacterial species and pathways associated with prodromal and recently onset PD.ResultsWe observed depletion of several strict anaerobes associated with reduced inflammation among participants with PD or features of prodromal PD. A microbiome‐based classifier had moderate accuracy (area under the curve [AUC] = 0.76 for species and 0.74 for pathways) to discriminate between recently onset PD cases and controls. These taxonomic shifts corresponded with functional shifts indicative of carbohydrate source preference. Similar, but less marked, changes were observed in participants with features of prodromal PD, in both microbial features and functions.InterpretationPD and features of prodromal PD were associated with similar changes in the gut microbiome. These findings suggest that changes in the microbiome could represent novel biomarkers for the earliest phases of PD. ANN NEUROL 2023

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Healthy Controls and Controls With Constipationmentioning

confidence: 99%

“…The supplemental questionnaire used in this study was developed for the Micro-N study and has been described in detail and published previously. 46…”

Section: Assessment Of Covariatesmentioning

confidence: 99%

See 2 more Smart Citations

Metagenomics of the Gut Microbiome in Parkinson's Disease: Prodromal Changes

Palacios

Wilkinson²,

Bjørnevik

et al. 2023

Annals of Neurology

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show abstract

“…The field is currently lacking large, accessible datasets focused on in vivo drug-microbiome interactions. At present, high throughput ex vivo studies (Javdan et al, 2020;Zimmermann et al, 2019a) or general observation microbiome studies (Everett et al, 2021;Huttenhower et al, 2012;Proctor et al, 2019) are the best sources of data for ML. A few databases have also been built to collect disease-microbiome or drug-microbiome interactions in a single place (Janssens et al, 2018;Sun et al, 2018).…”

Section: The Power Of Predictionmentioning

confidence: 99%