A multi-herb-combined remedy to overcome hyper-inflammatory response by reprogramming transcription factor profile and shaping monocyte subsets

De, Gejing; Chen, Apeng; Zhao, Qinghe; Xie, Ran; Wang, Chaoxi; Li, Meng; Zhao, Haiyu; Gu, Xinru; McCarl, Lauren; Zhang, Fangbo; Cai, Wei; Yang, Miyi; Lin, Pei‐Hui; Liu, Shaorong; Bian, Baolin

doi:10.1016/j.phrs.2021.105617

Cited by 9 publications

(3 citation statements)

References 61 publications

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“…Traditional Chinese medicine and its extracts have potential in treating periodontitis ( 21 ). Oroxylin A is the primary active ingredient in S. baicalensis , with anti-inflammatory, antitumor, antioxidant, vascular protection and other pharmacological effects ( 22 , 23 ).…”

Section: Discussionmentioning

confidence: 99%

Oroxylin A inhibits inflammatory cytokines in periodontitis via HO‑1

Wang,

Jiang

et al. 2024

Mol Med Rep

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Periodontal disease is a common infectious disease that can lead to the loss of teeth. Hower how to effectively suppress the inflammation with medication is unclear. The aim of the present study was to investigate the anti-inflammatory effect of Oroxylin A in periodontitis and its potential role through heme oxygenase-1 (HO-1). Primary rat gingival fibroblasts (RGFs) were cultured using the tissue block method and identified by immunofluorescence. Following lipopolysaccharide (LPS) stimulation of RGFs, Oroxylin A was administered at 50, 100, 200 or 400 µg/ml. Reverse transcription-quantitative PCR was used to assess mRNA expression of cyclooxygenase (COX)-2, TNF-α, RANKL and osteoprotegerin (OPG). Western blotting was used to detect protein expression levels of COX −2, TNF-α, RANKL and OPG. Following HO-1 knockdown, the same treatment was performed. The expression of COX-2 in rat gingival tissue was observed by immunohistochemistry. One-way analysis of variance and Student's t test were used for statistical analysis. Oroxylin A downregulated mRNA expression of COX-2, TNF-α, RANKL and OPG in LPS-induced RGFs. With increase of Oroxylin A dose, the expression of HO-1 was gradually upregulated. When HO-1 was knocked down, Oroxylin A did not downregulate the expression of COX-2, TNF-α, RANKL and OPG in LPS-induced RGFs. Immunohistochemical results showed that expression of COX-2 was downregulated by Oroxylin A, and the expression of TNF-α, RANKL and OPG were also downregulated. Oroxylin A decreased expression of inflammatory cytokines in LPS-induced RGFs and had a good inhibitory effect on periodontitis in rats.

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Section: Discussionmentioning

confidence: 99%

Oroxylin A inhibits inflammatory cytokines in periodontitis via HO‑1

Wang,

Jiang

et al. 2024

Mol Med Rep

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“…Among these TCMs, several classical tonifying medicines, including Eucommia ulmoides Oliver bark (EUOB), Eucommia ulmoides Oliver male flower (EUOF), Panax notoginseng (PN), and Syzygium aromaticum (SA), have attracted our research interests and attention (29)(30)(31)(32). The traditional medicine theory postulates that the herbal combination has better therapeutic efficacies than single herbs by rational design (33)(34)(35). This theory is widely accepted and has become an important research tool for designing novel therapeutics for multiple diseases, such as digestive, neurological, and respiratory diseases (15,33).…”

Section: Introductionmentioning

confidence: 99%

Feasibility study on the use of “Qi-tonifying medicine compound” as an anti-fatigue functional food ingredient based on network pharmacology and molecular docking

Cao

et al. 2023

Front. Nutr.

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IntroductionFatigue has attracted broad attention in recent years due to its high morbidity rates. The use of functional foods to relieve fatigue-associated symptoms is becoming increasingly popular and has achieved relatively good results. In this study, network pharmacology and molecular docking strategies were used to establish the material basis and mechanisms of Chinese herbal compounds in fatigue treatment. According to traditional medicine theories and relevant guidance documents published by the Chinese Ministry of Health, four herbal medicines, including Eucommia ulmoides Oliver bark, Eucommia ulmoides Oliver male flower, Panax notoginseng, and Syzygium aromaticum (EEPS), were selected to constitute the anti-fatigue herbal compound that may be suitable as functional food ingredients.MethodsThe major active ingredients in EEPS were identified via comprehensive literature search and Traditional Chinese Medicine Systems Pharmacology database search. Corresponding targets for these ingredients were predicted using SwissTargetPrediction. The network was constructed using Cytoscape 3.9.1 to obtain key ingredients. Prediction of absorption, distribution, metabolism, excretion and toxicity properties was performed using the ADMETIab 2.0 database. The anti-fatigue targets were retrieved from GeneCards v5.13, OMIM, TTD and DisGeNET 7.0 databases. Then, the potential targets of EEPS in fatigue treatment were screened through a Venn diagram. A protein–protein interaction (PPI) network of these overlapping targets was constructed, and the hub targets in the network selected through topological screening. Gene Ontology and KEGG pathway enrichment analyses were performed using the DAVID database and the bioinformatics online platform. Finally, AutoDock tools were used to verify the binding capacity between the key active ingredients and the core targets.Results and DiscussionThis study identified the active ingredients and potential molecular mechanisms of EEPS in fatigue treatment, which will provide a foundation for future research on applications of herbal medicines in the functional food industry.

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“…Thus, the need for alternative therapeutic approaches for treating breast inflammation is indicated. Herbs from traditional Chinese medicine (TCM), both in formulations and as a single active ingredient, have shown low resistance to the dual regulation of a bacterium and a host by altering the permeability of biological membranes and ion channels and inhibiting bacterial enzyme activities [ [20] , [21] , [22] ]. For a more effective and early prevention and treatment of LM and to reduce its incidence and the progression of breast abscesses, the use of herbal medicines from TCM can be a potential treatment approach [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%