The transcriptome landscapes of citrus leaf in different developmental stages

Ribeiro, Cauê; Xu, Jin; Teper, Doron; Lee, Donghwan; Wang, Nian

doi:10.1007/s11103-021-01154-8

Cited by 9 publications

(5 citation statements)

References 104 publications

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“…The transcriptome landscapes of Citrus sinensis leaf indicated that DEGs related to PS I and II, ferredoxin, ATP synthase, cytb 6 f, Rubisco, and GAPDH were predominantly upregulated in both transition and mature leaves compared with immature leaves. The same trend was found with glucose levels [16]. The photosynthetic capacity decreases with leaf senescence mainly because of the loss of Rubisco [7].…”

Section: Introductionsupporting

confidence: 77%

Transcription Profiles Reveal Age-Dependent Variations of Photosynthetic Properties and Sugar Metabolism in Grape Leaves (Vitis vinifera L.)

You

Nie

et al. 2022

IJMS

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Leaves, considered as the ‘source’ organs, depend on the development stages because of the age-dependent photosynthesis and assimilation of leaves. However, the molecular mechanisms of age-dependent limitations on the function of leaves are seldom reported. In the present study, the photosynthesis-related characteristics and photoassimilates were investigated in grape leaves at six different age groups (Ll to L6) at micro-morphological, biochemical, and molecular levels. These results showed lower expression levels of genes associated with stomatal development, and chl biosynthesis resulted in fewer stomata and lowered chlorophyll a/b contents in L1 when compared to L3 and L5. The DEGs between L5 and L3/L1 were largely distributed at stomatal movement, carbon fixation, and sucrose and starch metabolism pathways, such as STOMATAL ANION CHANNEL PROTEIN 1 (SLAC1), FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE (FBA1), SUCROSE-PHOSPHATE SYNTHASE (SPP1), and SUCROSE-PHOSPHATE PHOSPHATASE (SPS2, 4). These genes could be major candidate genes leading to increased photosynthesis capacity and sugar content in L5. The accumulation of starch grains in the chloroplast and palisade tissue of L5 and higher transcription levels of genes related to starch biosynthesis in L5 further supported the high ability of L5 to produce photoassimilates. Hence, our results provide insights for understanding different photosynthetic functions in age-dependent leaves in grape plants at the molecular level.

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Section: Introductionsupporting

confidence: 77%

Transcription Profiles Reveal Age-Dependent Variations of Photosynthetic Properties and Sugar Metabolism in Grape Leaves (Vitis vinifera L.)

You

Nie

et al. 2022

IJMS

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“…The 320 bp core region of the 35S promoter in the CRISPR/Cas9 construct was replaced with either the Citrus sinensis ubiquitin (CsUbi) or Cestrum yellow leaf curling virus (CmYLCV) promoter ( Supplementary Data 1 ). The CsUbi promoter (Cs4g11190) was selected through mining of citrus RNAseq data ( Ribeiro et al, 2021 ). Cs4g11190 is highly expressed in various stages of leaf development ( Supplementary Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

Highly Efficient Generation of Canker-Resistant Sweet Orange Enabled by an Improved CRISPR/Cas9 System

Huang

Wang

2022

Front. Plant Sci.

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Sweet orange (Citrus sinensis) is the most economically important species for the citrus industry. However, it is susceptible to many diseases including citrus bacterial canker caused by Xanthomonas citri subsp. citri (Xcc) that triggers devastating effects on citrus production. Conventional breeding has not met the challenge to improve disease resistance of sweet orange due to the long juvenility and other limitations. CRISPR-mediated genome editing has shown promising potentials for genetic improvements of plants. Generation of biallelic/homozygous mutants remains difficult for sweet orange due to low transformation rate, existence of heterozygous alleles for target genes, and low biallelic editing efficacy using the CRISPR technology. Here, we report improvements in the CRISPR/Cas9 system for citrus gene editing. Based on the improvements we made previously [dicot codon optimized Cas9, tRNA for multiplexing, a modified sgRNA scaffold with high efficiency, citrus U6 (CsU6) to drive sgRNA expression], we further improved our CRISPR/Cas9 system by choosing superior promoters [Cestrum yellow leaf curling virus (CmYLCV) or Citrus sinensis ubiquitin (CsUbi) promoter] to drive Cas9 and optimizing culture temperature. This system was able to generate a biallelic mutation rate of up to 89% for Carrizo citrange and 79% for Hamlin sweet orange. Consequently, this system was used to generate canker-resistant Hamlin sweet orange by mutating the effector binding element (EBE) of canker susceptibility gene CsLOB1, which is required for causing canker symptoms by Xcc. Six biallelic Hamlin sweet orange mutant lines in the EBE were generated. The biallelic mutants are resistant to Xcc. Biallelic mutation of the EBE region abolishes the induction of CsLOB1 by Xcc. This study represents a significant improvement in sweet orange gene editing efficacy and generating disease-resistant varieties via CRISPR-mediated genome editing. This improvement in citrus genome editing makes genetic studies and manipulations of sweet orange more feasible.

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“…Persian lime (Citrus latifolia) leaf samples were obtained from an experimental orchard established at the Campo Experimental Ixtacuaco belonging to the Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), located at the coordinates 20 • 2 35.48" N and 97 • 5 52.60" W. Persian lime trees grafted on Citrumelo Swingle rootstock were 5 years old at the time of sampling. A total of three visually healthy and physiologically mature (V6 according to the [71] scale) leaves from three asymptomatic trees (absence of visible symptoms of HLB) and three leaves from three different HLB-symptomatic trees (spotted leaves, starch accumulation in veins, etc.) were collected (Figure 1).…”

Section: Plant Materials and Experimental Designmentioning

confidence: 99%

Insights into the Molecular Basis of Huanglongbing Tolerance in Persian Lime (Citrus latifolia Tan.) through a Transcriptomic Approach

Estrella‐Maldonado

González-Cruz

Matilde-Hernández

et al. 2023

IJMS

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Huanglongbing (HLB) is a vascular disease of Citrus caused by three species of the α-proteobacteria “Candidatus Liberibacter”, with “Candidatus Liberibacter asiaticus” (CLas) being the most widespread and the one causing significant economic losses in citrus-producing regions worldwide. However, Persian lime (Citrus latifolia Tanaka) has shown tolerance to the disease. To understand the molecular mechanisms of this tolerance, transcriptomic analysis of HLB was performed using asymptomatic and symptomatic leaves. RNA-Seq analysis revealed 652 differentially expressed genes (DEGs) in response to CLas infection, of which 457 were upregulated and 195 were downregulated. KEGG analysis revealed that after CLas infection, some DEGs were present in the plant–pathogen interaction and in the starch and sucrose metabolism pathways. DEGs present in the plant–pathogen interaction pathway suggests that tolerance against HLB in Persian lime could be mediated, at least partly, by the ClRSP2 and ClHSP90 genes. Previous reports documented that RSP2 and HSP90 showed low expression in susceptible citrus genotypes. Regarding the starch and sucrose metabolism pathways, some genes were identified as being related to the imbalance of starch accumulation. On the other hand, eight biotic stress-related genes were selected for further RT-qPCR analysis to validate our results. RT-qPCR results confirmed that symptomatic HLB leaves had high relative expression levels of the ClPR1, ClNFP, ClDR27, and ClSRK genes, whereas the ClHSL1, ClRPP13, ClPDR1, and ClNAC genes were expressed at lower levels than those from HLB asymptomatic leaves. Taken together, the present transcriptomic analysis contributes to the understanding of the CLas-Persian lime interaction in its natural environment and may set the basis for developing strategies for the integrated management of this important Citrus disease through the identification of blanks for genetic improvement.

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The transcriptome landscapes of citrus leaf in different developmental stages

Cited by 9 publications

References 104 publications

Transcription Profiles Reveal Age-Dependent Variations of Photosynthetic Properties and Sugar Metabolism in Grape Leaves (Vitis vinifera L.)

Transcription Profiles Reveal Age-Dependent Variations of Photosynthetic Properties and Sugar Metabolism in Grape Leaves (Vitis vinifera L.)

Highly Efficient Generation of Canker-Resistant Sweet Orange Enabled by an Improved CRISPR/Cas9 System

Insights into the Molecular Basis of Huanglongbing Tolerance in Persian Lime (Citrus latifolia Tan.) through a Transcriptomic Approach

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