Analyses of viral genomes for G-quadruplex forming sequences reveal their correlation with the type of infection

Bohálová, Natália; Cantara, Alessio; Bartas, Martin; Kaura, Patrik; Šťastný, Jiří; Pečínka, Petr; Fojta, Miroslav; Mergny, Jean‐Louis; Brázda, Václav

doi:10.1016/j.biochi.2021.03.017

Cited by 35 publications

(26 citation statements)

References 77 publications

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“…This suggests that controlling their own rate of translation might be a common strategy whereby, at least latent, viral messages evade the immune system. In agreement with this hypothesis is the observation that viruses causing persistent infections in Metazoa hosts are significantly enriched with putative G-quadruplex forming sequences (PQS), whereas viruses causing acute infections are significantly depleted in PQS ( Bohálová et al, 2021 ).…”

Section: Discussionmentioning

confidence: 61%

The different activities of RNA G-quadruplex structures are controlled by flanking sequences

et al. 2021

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The role of G-quadruplex (G4) RNA structures is multifaceted and controversial. Here, we have used as a model the EBV-encoded EBNA1 and the Kaposi’s sarcoma-associated herpesvirus (KSHV)-encoded LANA1 mRNAs. We have compared the G4s in these two messages in terms of nucleolin binding, nuclear mRNA retention, and mRNA translation inhibition and their effects on immune evasion. The G4s in the EBNA1 message are clustered in one repeat sequence and the G4 ligand PhenDH2 prevents all G4-associated activities. The RNA G4s in the LANA1 message take part in similar multiple mRNA functions but are spread throughout the message. The different G4 activities depend on flanking coding and non-coding sequences and, interestingly, can be separated individually. Together, the results illustrate the multifunctional, dynamic and context-dependent nature of G4 RNAs and highlight the possibility to develop ligands targeting specific RNA G4 functions. The data also suggest a common multifunctional repertoire of viral G4 RNA activities for immune evasion.

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Section: Discussionmentioning

confidence: 61%

The different activities of RNA G-quadruplex structures are controlled by flanking sequences

et al. 2021

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“…The bioinformation analysis reveal that the PQS frequency, motif size, loop length, and nucleotide compositions show a correlation between the genome of virus and host, suggesting a coevolution of virus and host. 79 , 130 , 131 …”

Section: The Role Of G-quadruplex In Virologymentioning

confidence: 99%

G-Quadruplexes in Neurobiology and Virology: Functional Roles and Potential Therapeutic Approaches

Huang

Zhou

2021

JACS Au

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A G-quadruplex (G4) is a four-stranded nucleic acid secondary structure maintained by Hoogsteen hydrogen bonds established between four guanines. Experimental studies and bioinformatics predictions support the hypothesis that these structures are involved in different cellular functions associated with both DNA and RNA processes. An increasing number of diseases have been shown to be associated with abnormal G4 regulation. Here, we describe the existence of G4 and then discuss G4-related pathogenic mechanisms in neurodegenerative diseases and the viral life cycle. Furthermore, we focus on the role of G4s in the design of antiviral therapy and neuropharmacology, including G4 ligands, G4-based aptamers, G4-related proteins, and CRISPR-based sequence editing, along with a discussion of limitations and insights into the prospects of this unusual nucleic acid secondary structure in therapeutics. Finally, we highlight progress and challenges in this field and the potential G4-related research fields.

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“…Viruses belonging to almost all Baltimore classes have been found to be enriched in highly conserved and putative G4-forming sequences (PQSs) located in significant position within the viral genome [14,15]. The development and optimization of bioinformatics tools to detect G4 motifs have highly accelerated the identification of viral PQSs; interestingly, most of the identified sequences were proved to actually fold into G4s in vitro [16].…”

Section: Introductionmentioning

confidence: 99%

G-Quadruplex Targeting in the Fight against Viruses: An Update

Ruggiero

Zanin

Terreri

et al. 2021

IJMS

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G-quadruplexes (G4s) are noncanonical nucleic acid structures involved in the regulation of key cellular processes, such as transcription and replication. Since their discovery, G4s have been mainly investigated for their role in cancer and as targets in anticancer therapy. More recently, exploration of the presence and role of G4s in viral genomes has led to the discovery of G4-regulated key viral pathways. In this context, employment of selective G4 ligands has helped to understand the complexity of G4-mediated mechanisms in the viral life cycle, and highlighted the possibility to target viral G4s as an emerging antiviral approach. Research in this field is growing at a fast pace, providing increasing evidence of the antiviral activity of old and new G4 ligands. This review aims to provide a punctual update on the literature on G4 ligands exploited in virology. Different classes of G4 binders are described, with emphasis on possible antiviral applications in emerging diseases, such as the current COVID-19 pandemic. Strengths and weaknesses of G4 targeting in viruses are discussed.

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Analyses of viral genomes for G-quadruplex forming sequences reveal their correlation with the type of infection

Cited by 35 publications

References 77 publications

The different activities of RNA G-quadruplex structures are controlled by flanking sequences

The different activities of RNA G-quadruplex structures are controlled by flanking sequences

G-Quadruplexes in Neurobiology and Virology: Functional Roles and Potential Therapeutic Approaches

G-Quadruplex Targeting in the Fight against Viruses: An Update

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