Functional characterization of Kid-Kis and MazF-MazE in Sf9 cells and Mythimna separata embryos

Zhang, Qiuyuan; Yicheng, Zhou; Li, Yunfei; Ali, Bahar; Zhu, Zhihui

doi:10.1016/j.pestbp.2021.104814

Cited by 1 publication

(1 citation statement)

References 29 publications

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“…The Kid toxin acts as a sequence-specific ribonuclease and bacterial DNA gyrase inhibitor, leading to the SOS response induction, cell cycle arrest, and growth inhibition in bacteria [ 177 ]. This toxin was also shown to inhibit cell proliferation in a wide range of eukaryotic cells, including yeast, embryonic cells of Xenopus laevis amphibia [ 166 ], Mythimna separata insect cells [ 178 ], rabbit reticulocytes [ 179 ], Spodoptera frugiperda insect cells (Sf9) [ 178 ], and human HeLa and SW480 cells [ 166 ]. In the kis-kid -based anticancer strategy, again, the differences in gene expression between healthy and malignant cells were employed.…”

Section: Toxin-antitoxin Systems—practical Application In Medicinementioning

confidence: 99%

Bacterial Toxin-Antitoxin Systems’ Cross-Interactions—Implications for Practical Use in Medicine and Biotechnology

Boss

Kędzierska

2023

Toxins

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Toxin-antitoxin (TA) systems are widely present in bacterial genomes. They consist of stable toxins and unstable antitoxins that are classified into distinct groups based on their structure and biological activity. TA systems are mostly related to mobile genetic elements and can be easily acquired through horizontal gene transfer. The ubiquity of different homologous and non-homologous TA systems within a single bacterial genome raises questions about their potential cross-interactions. Unspecific cross-talk between toxins and antitoxins of non-cognate modules may unbalance the ratio of the interacting partners and cause an increase in the free toxin level, which can be deleterious to the cell. Moreover, TA systems can be involved in broadly understood molecular networks as transcriptional regulators of other genes’ expression or modulators of cellular mRNA stability. In nature, multiple copies of highly similar or identical TA systems are rather infrequent and probably represent a transition stage during evolution to complete insulation or decay of one of them. Nevertheless, several types of cross-interactions have been described in the literature to date. This implies a question of the possibility and consequences of the TA system cross-interactions, especially in the context of the practical application of the TA-based biotechnological and medical strategies, in which such TAs will be used outside their natural context, will be artificially introduced and induced in the new hosts. Thus, in this review, we discuss the prospective challenges of system cross-talks in the safety and effectiveness of TA system usage.

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Section: Toxin-antitoxin Systems—practical Application In Medicinementioning

confidence: 99%

Bacterial Toxin-Antitoxin Systems’ Cross-Interactions—Implications for Practical Use in Medicine and Biotechnology

Boss

Kędzierska

2023

Toxins

View full text Add to dashboard Cite

show abstract

Functional characterization of Kid-Kis and MazF-MazE in Sf9 cells and Mythimna separata embryos

Cited by 1 publication

References 29 publications

Bacterial Toxin-Antitoxin Systems’ Cross-Interactions—Implications for Practical Use in Medicine and Biotechnology

Bacterial Toxin-Antitoxin Systems’ Cross-Interactions—Implications for Practical Use in Medicine and Biotechnology

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