Apelin-13 prevents apoptosis in the cochlear tissue of noise-exposed rat via Sirt-1 regulation

Khoshsirat, Shahrokh; Abbaszadeh, Hojjat-Allah; Peyvandi, Ali Asghar; Heidari, Fatemeh; Peyvandi, Maryam; Simani, Leila; Niknazar, Somayeh

doi:10.1016/j.jchemneu.2021.101956

Cited by 22 publications

(11 citation statements)

References 64 publications

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“…In addition, cellular oxidative stress might contribute to dysregulation of normal SIRT1 functioning ( Singh et al, 2018 ). The cochleae ( Xiong et al, 2017 ; Khoshsirat et al, 2021 ) and auditory cortex ( Chen et al, 2020 ) of the model of NIHL exhibited a decrease in SIRT1 expression after noise exposure, and the structure and function were aberrant. Treatment with the SIRT1 activator enhanced SIRT1 activity, reduced reactive species, promoted recovery of auditory function, and protected auditory cortex neuron cells and hair cells.…”

Section: Discussionmentioning

confidence: 99%

Involvement of the SIRT1/PGC-1α Signaling Pathway in Noise-Induced Hidden Hearing Loss

Liu

Jiang

et al. 2022

Front. Physiol.

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Objective: To establish an animal model of noise-induced hidden hearing loss (NIHHL), evaluate the dynamic changes in cochlear ribbon synapses and cochlear hair cell morphology, and observe the involvement of the SIRT1/PGC-1α signaling pathway in NIHHL.Methods: Male guinea pigs were randomly divided into three groups: control group, noise exposure group, and resveratrol treatment group. Each group was divided into five subgroups: the control group and 1 day, 1 week, 2 weeks, and 1 month post noise exposure groups. The experimental groups received noise stimulation at 105 dB SPL for 2 h. Hearing levels were examined by auditory brainstem response (ABR). Ribbon synapses were evaluated by inner ear basilar membrane preparation and immunofluorescence. The cochlear morphology was observed using scanning electron microscopy. Western blotting analysis and immunofluorescence was performed to assess the change of SIRT1/PGC-1α signaling. Levels of superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), ATP and SIRT1 activity were measured using commercial testing kits.Results: In the noise exposure group, hearing threshold exhibited a temporary threshold shift (TTS), and amplitude of ABR wave I decreased irreversibly. Ribbon synapse density decreased after noise exposure, and the stereocilia were chaotic and then returned to normal. The expression and activity of SIRT1 and PGC-1α protein was lower than that in the control group. SOD, CAT and ATP were also influenced by noise exposure and were lower than those in the control group, but MDA showed no statistical differences compared with the control group. After resveratrol treatment, SIRT1 expression and activity showed a significant increase after noise exposure, compared with the noise exposure group. In parallel, the PGC-1α and antioxidant proteins were also significantly altered after noise exposure, compared with the noise exposure group. The damage to the ribbon synapses and the stereocilia were attenuated by resveratrol as well. More importantly, the auditory function, especially ABR wave I amplitudes, was also promoted in the resveratrol treatment group.Conclusion: The SIRT1/PGC-1α signaling pathway and oxidative stress are involved in the pathogenesis of NIHHL and could be potential therapeutical targets in the future.

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Section: Discussionmentioning

confidence: 99%

Involvement of the SIRT1/PGC-1α Signaling Pathway in Noise-Induced Hidden Hearing Loss

Liu

Jiang

et al. 2022

Front. Physiol.

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“…4G), which was consistent with previous studies. 53,54 Intriguingly, histology and the corresponding quantitative data revealed that SGN density was significantly enhanced in the low-frequency area (4-8 kHz) after EGCG@TDNs treatment, indicating the prevention of SGN apoptosis by EGCG@TDNs (Fig. 4H).…”

Section: Characterization Of Egcg@tdnsmentioning

confidence: 86%

Epigallocatechin gallate-loaded tetrahedral DNA nanostructures as a novel inner ear drug delivery system

Chen

Liu

et al. 2022

Nanoscale

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“…For example, apelin could be a potential therapeutic factor for noise-induced hearing loss. Khoshirat et al demonstrated that apelin inhibits intrinsic apoptosis through the growth of Sirt-1 in cochlear tissue, and, as a result, it decreases caspase-3 expression and the Bax/Bcl-2 ratio, which leads to an improvement in hearing function in rats [102]. Yin et al observed the anti-apoptotic effect of apelin in mouse HC explants and the mouse auditory cell line HEI-OC1.…”

Section: Other Tissues and Cellsmentioning

confidence: 99%

“…In contrast, after 48 h of incubation, apelin had a stimulating effect on the gene expression of caspase-3 in Caco-2 cells. On the basis of many scientific studies, it has been suggested that apelin impacts the Bax/Bcl-2 ratio [96,102,103]. However, in rat stomach and jejunum cells, apelin increases the expression of caspase-3, which is associated with DNA damage [43].…”

Section: Pro-apoptotic Effect Of Apelinergic Systemmentioning

confidence: 99%

The Apelinergic System: Apelin, ELABELA, and APJ Action on Cell Apoptosis: Anti-Apoptotic or Pro-Apoptotic Effect?

Respekta

Pich

Dawid

et al. 2022

Cells

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The apelinergic system comprises two peptide ligands, apelin and ELABELA, and their cognate G-protein-coupled receptor, the apelin receptor APJ. Apelin is a peptide that was isolated from bovine stomach extracts; the distribution of the four main active forms, apelin-36, -17, -13, and pyr-apelin-13 differs between tissues. The mature form of ELABELA-32 can be transformed into forms called ELABELA-11 or -21. The biological function of the apelinergic system is multifaceted, and includes the regulation of angiogenesis, body fluid homeostasis, energy metabolism, and functioning of the cardiovascular, nervous, respiratory, digestive, and reproductive systems. This review summarises the mechanism of the apelinergic system in cell apoptosis. Depending on the cell/tissue, the apelinergic system modulates cell apoptosis by activating various signalling pathways, including phosphoinositide 3-kinase (PI3K), extracellular signal-regulated protein kinase (ERK1/2), protein kinase B (AKT), 5’AMP-activated protein kinase(AMPK), and protein kinase A (PKA). Apoptosis is critically important during various developmental processes, and any dysfunction leads to pathological conditions such as cancer, autoimmune diseases, and developmental defects. The purpose of this review is to present data that suggest a significant role of the apelinergic system as a potential agent in various therapies.

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Apelin-13 prevents apoptosis in the cochlear tissue of noise-exposed rat via Sirt-1 regulation

Cited by 22 publications

References 64 publications

Involvement of the SIRT1/PGC-1α Signaling Pathway in Noise-Induced Hidden Hearing Loss

Involvement of the SIRT1/PGC-1α Signaling Pathway in Noise-Induced Hidden Hearing Loss

Epigallocatechin gallate-loaded tetrahedral DNA nanostructures as a novel inner ear drug delivery system

The Apelinergic System: Apelin, ELABELA, and APJ Action on Cell Apoptosis: Anti-Apoptotic or Pro-Apoptotic Effect?

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