Integrative Proteomic and Metabolomic Analysis Reveals Metabolic Phenotype in Mice With Cardiac-Specific Deletion of Natriuretic Peptide Receptor A

Pan, Chaoyun; Niu, Yan; Zhang, Xiaomeng; Zhang, Jing; Wang, Xihui; Shen, Xiaoye; Chen, Baoying; Yu, Jun

doi:10.1016/j.mcpro.2021.100072

Cited by 7 publications

(11 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, 9 of the top 10 terms are associated with the metabolic process which is in good agreement with our past findings that NPs may play a pivotal role in metabolic functions. [27][28][29] Bubble plots show these metabolic processes in BP bars, the bubble size shows the counts of genes, the number of enriched genes are listed in brackets on the left, and the color of the bubble represents the P-value.…”

Section: Resultsmentioning

confidence: 99%

“…Our previous result showed that there are greatly altered expression profiles in the myocardium tested by RNA-seq 27 and greatly altered metabolic patterns in mice with cardiac-specific deletion of NPRA such as COX, ATP5J2 (mitochondrial membrane ATP synthase), PCr, 2-keto-glutaramic acid (a-KG), etc. 28 Besides, distinguished altered metabolites were also found in HF patients with a remarkably lower cGMP/BNP ratio. 29 However, the molecular mechanism that could account for this observation induced by disabled natriuretic peptide signalling remains to be investigated.…”

Section: Introductionmentioning

confidence: 94%

See 1 more Smart Citation

The landscape of N6-methyladenosine modification patterns and altered transcript profiles in the cardiac-specific deletion of natriuretic peptide receptor A

Shen¹,

Pan

Zhang

et al. 2023

Mol. Omics

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

The landscape of N6-methyladenosine modification patterns and altered transcript profiles in the cardiac-specific deletion of natriuretic peptide receptor A

Shen¹,

Pan

Zhang

et al. 2023

Mol. Omics

Self Cite

View full text Add to dashboard Cite

show abstract

“…The importance of metabolic reprogramming in the onset and progression of cardiovascular disease has been well characterized (Chang et al, 2021). Since correlation plays an integral part in disease progression, we assume it may be a signi cant cause.…”

Section: Discussionmentioning

confidence: 99%

Integrated proteomics and metabolomics analysis of cardiac-tissue in mice with sepsis

Xiao

Li³

et al. 2022

Preprint

View full text Add to dashboard Cite

Introduction: Sepsis remains a devastating disease with a high fatality rate in most nations. Heart failure accounts for around half of all deaths in sepsis patients. Because of the peculiarities of cardiac tissue, the specific mechanism of sepsis-induced cardiac dysfunction remains largely unknown. Objectives: The goal of this study was to develop a more complete understanding of the molecular pathways of septic cardiomyopathy by doing an integrated proteome and metabolomics analysis on a sepsis-infected mouse model. Methods: The CLP mice were utilized as a model in this study to investigate the impact of septic cardiomyopathy on the molecular changes in heart tissues through combining metabolomics and proteomics studies. Results: The present findings suggest that 118 proteins (75-upregulated and 43-downregulated) and 138 metabolites (124-upregulated and 14-downregulated) were significantly altered. Additionally, these compounds play a significant role in biological processes, such as platelet activation, mineral absorption, drug metabolism, terpenoid backbone biosynthesis, and butanoate metabolism. The comprehensive analysis discovered that prostaglandin H2, prostaglandin I2, thromboxane A2, and L-Glutamine might play essential roles in combating sepsis-induced heart dysfunction. Conclusions: Overall, this work adds to our understanding of the implications of sepsis-induced cardiac dysfunction and may lead to identifying novel potential biomarkers and a better understanding of sepsis pathophysiology.

show abstract

“…UQCR10 has been involved in the mitochondrial oxidative phosphorylation and myocardial contraction in cardiomyocytes. 37 MrpS28 encodes a mitochondrial ribosomal protein, which is essential for mitochondrial ribosomal assembly, mitochondrial translation and oxidative phosphorylation. 38 NDUFB6 , a mitochondrial gene, plays a major role in reactive oxygen species (ROS) production.…”

Section: Discussionmentioning

confidence: 99%

Integrative Bioinformatics Analysis Revealed Mitochondrial Defects Underlying Hypoplastic Left Heart Syndrome

Zhao¹,

Liu²,

Xu³

et al. 2021

IJGM

View full text Add to dashboard Cite

Background Hypoplastic left heart syndrome (HLHS) is one of the most complex congenital cardiac malformations, and the molecular mechanism of heart failure (HF) in HLHS is still elusive. Methods Integrative bioinformatics analysis was performed to unravel the underlying genes and mechanisms involved in HF in HLHS. Microarray dataset GSE23959 was screened out for the differentially expressed genes (DEGs), after which the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses were carried out using the Metascape. The protein–protein interaction (PPI) network was generated, and the modules and hub genes were identified with the Cytoscape-plugin. And the integrated network of transcription factor (TF)-DEGs and miRNA-DEGs was constructed, respectively. Results A total of 210 DEGs were identified, including 135 up-regulated and 75 down-regulated genes. The functional enrichment analysis of DEGs pointed towards the mitochondrial-related biological processes, cellular components, molecular functions and signaling pathways. A PPI network was constructed including 155 nodes as well as 363 edges. And 15 hub genes, such as NDUFB6, UQCRQ, SDHD, ATP5H , were identified based on three topological analysis methods and mitochondrial components and functions were the most relevant. Furthermore, by integrating network interaction construction, 23 TFs (NFKB1, RELA, HIF1A, VHL, GATA1, PPAR-γ, etc.) as well as several miRNAs (hsa-miR-155-5p, hsa-miR-191-5p, hsa-mir-124-3p, hsa-miR-1-3p, etc.) were detected and indicated the possible involvement of NF-κB signaling pathways in mitochondrial dysfunction in HLHS. Conclusion The present study applied the integrative bioinformatics analysis and revealed the mitochondrial-related key genes, regulatory pathways, TFs and miRNAs underlying the HF in HLHS, which improved the understanding of disease mechanisms and the development of novel therapeutic targets.

show abstract

Integrative Proteomic and Metabolomic Analysis Reveals Metabolic Phenotype in Mice With Cardiac-Specific Deletion of Natriuretic Peptide Receptor A

Cited by 7 publications

References 43 publications

The landscape of N6-methyladenosine modification patterns and altered transcript profiles in the cardiac-specific deletion of natriuretic peptide receptor A

The landscape of N6-methyladenosine modification patterns and altered transcript profiles in the cardiac-specific deletion of natriuretic peptide receptor A

Integrated proteomics and metabolomics analysis of cardiac-tissue in mice with sepsis

Integrative Bioinformatics Analysis Revealed Mitochondrial Defects Underlying Hypoplastic Left Heart Syndrome

Contact Info

Product

Resources

About