Light and CO2 Modulate the Accumulation and Localization of Phenolic Compounds in Barley Leaves

Hunt, Lena; Klem, Karel; Lhotáková, Zuzana; Vosolsobě﻿﻿﻿, Stanislav; Oravec, Michal; Urban, Otmar; Špunda, Vladimı́r; Albrechtová, Jana

doi:10.3390/antiox10030385

Cited by 15 publications

(17 citation statements)

References 71 publications

(66 reference statements)

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“…Hexoses were negatively correlated with ABA in the RDA graph (Figure 5). Moreover, the genotypes Barke and Bojos were previously characterized in our recent work on the accumulation of phenolic compounds in barley [20]. We found that Barke, considered an oxidative stress sensitive genotype, accumulated more hydroxybenzoic acids, while Bojos, an oxidative stress resistant genotype, accumulated more hydroxycinnamic acids.…”

Section: Discussionmentioning

confidence: 59%

“…HXK is also involved in the downregulation of photosynthesis under elevated [CO 2 ] when plants lack sufficient sinks for the products of photosynthesis [15]. This study looks at how experimentally manipulated [CO 2 ] and light conditions modulate both the stomatal density and stomatal conductance alongside physiological processes in two barley varieties differing in sensitivity to oxidative stress and phenolic compound profiles [20]-oxidative stress sensitive Barke and oxidative stress resistant Bojos. To measure stomatal density, we used a novel automated approach-a convolution neural network (CNN).…”

Section: Introductionmentioning

confidence: 99%

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Barley Genotypes Vary in Stomatal Responsiveness to Light and CO2 Conditions

Hunt

Fuksa

Klem

et al. 2021

Plants

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Changes in stomatal conductance and density allow plants to acclimate to changing environmental conditions. In the present paper, the influence of atmospheric CO2 concentration and light intensity on stomata were investigated for two barley genotypes—Barke and Bojos, differing in their sensitivity to oxidative stress and phenolic acid profiles. A novel approach for stomatal density analysis was used—a pair of convolution neural networks were developed to automatically identify and count stomata on epidermal micrographs. Stomatal density in barley was influenced by genotype, as well as by light and CO2 conditions. Low CO2 conditions resulted in increased stomatal density, although differences between ambient and elevated CO2 were not significant. High light intensity increased stomatal density compared to low light intensity in both barley varieties and all CO2 treatments. Changes in stomatal conductance were also measured alongside the accumulation of pentoses, hexoses, disaccharides, and abscisic acid detected by liquid chromatography coupled with mass spectrometry. High light increased the accumulation of all sugars and reduced abscisic acid levels. Abscisic acid was influenced by all factors—light, CO2, and genotype—in combination. Differences were discovered between the two barley varieties: oxidative stress sensitive Barke demonstrated higher stomatal density, but lower conductance and better water use efficiency (WUE) than oxidative stress resistant Bojos at saturating light intensity. Barke also showed greater variability between treatments in measurements of stomatal density, sugar accumulation, and abscisic levels, implying that it may be more responsive to environmental drivers influencing water relations in the plant.

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Section: Discussionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Barley Genotypes Vary in Stomatal Responsiveness to Light and CO2 Conditions

Hunt

Fuksa

Klem

et al. 2021

Plants

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“…However, we presume that the observed increase in AOX is at least partially caused by the significantly higher proportion of B-hydroxylated flavonoids (such as LUT, HFG, HSG) in extracts ( Figure 5 H) (AOX shows a higher correlation and dependency on the content of homoorientin derivatives, Supplementary Figure S2 vs. Figure S3 ) and hypothetically also due to higher content of FQA. The dominant contribution of blue light to the AOX capacity of PheCs may also be related to their localization since high PAR selectively accumulates PheCs in mesophyll cells, where they contribute to AOX rather than to UV-A shielding [ 18 ]. Nevertheless, the possible presence of other LMWA with strong antioxidant activity in extracts cannot be excluded.…”

Section: Discussionmentioning

confidence: 99%

“…PheCs play a role in plant defense against a wide range of stressors [ 16 ]. This large group of secondary metabolites includes photoprotective compounds, such as flavonoids and anthocyanins (important subclasses of PheCs), which absorb strong UV and PAR when localized in the epidermis [ 17 ] and parenchyma [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Regulation of Phenolic Compound Production by Light Varying in Spectral Quality and Total Irradiance

Pech

Volná

Hunt

et al. 2022

IJMS

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Photosynthetically active radiation (PAR) is an important environmental cue inducing the production of many secondary metabolites involved in plant oxidative stress avoidance and tolerance. To examine the complex role of PAR irradiance and specific spectral components on the accumulation of phenolic compounds (PheCs), we acclimated spring barley (Hordeum vulgare) to different spectral qualities (white, blue, green, red) at three irradiances (100, 200, 400 µmol m−2 s−1). We confirmed that blue light irradiance is essential for the accumulation of PheCs in secondary barley leaves (in UV-lacking conditions), which underpins the importance of photoreceptor signals (especially cryptochrome). Increasing blue light irradiance most effectively induced the accumulation of B-dihydroxylated flavonoids, probably due to the significantly enhanced expression of the F3′H gene. These changes in PheC metabolism led to a steeper increase in antioxidant activity than epidermal UV-A shielding in leaf extracts containing PheCs. In addition, we examined the possible role of miRNAs in the complex regulation of gene expression related to PheC biosynthesis.

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“…Ferulic and sinapic acid derivatives, acylated flavone glycosides, and hordatines, were found as drought‐responsive in leaves from young barley seedlings (Piasecka et al, 2017). Phenylpropanoid accumulation upon increasing CO 2 concentrations has also been observed (Hunt et al, 2021). Induction of specific phenylpropanoid branches during cold acclimation has been reported for winter cereals (NDong et al, 2003) and other plant species (Petridis et al, 2016; Solecka & Kacperska, 2003).…”

Section: Introductionmentioning

confidence: 91%

Untargeted metabotyping to study phenylpropanoid diversity in crop plants

Garibay-Hernández

Kessler

Józefowicz

et al. 2021

Physiologia Plantarum

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Plant genebanks constitute a key resource for breeding to ensure crop yield under changing environmental conditions. Because of their roles in a range of stress responses, phenylpropanoids are promising targets. Phenylpropanoids comprise a wide array of metabolites; however, studies regarding their diversity and the underlying genes are still limited for cereals. The assessment of barley diversity via genotyping-by-sequencing is in rapid progress. Exploring these resources by integrating genetic association studies to in-depth metabolomic profiling provides a valuable opportunity to study barley phenylpropanoid metabolism; but poses a challenge by demanding large-scale approaches. Here, we report an LC-PDA-MS workflow for barley high-throughput metabotyping. Without prior construction of a speciesspecific library, this method produced phenylpropanoid-enriched metabotypes with which the abundance of putative metabolic features was assessed across hundreds of samples in a single-processed data matrix. The robustness of the analytical performance was tested using a standard mix and extracts from two selected cultivars: Scarlett and Barke. The large-scale analysis of barley extracts showed (1) that barley flag leaf profiles were dominated by glycosylation derivatives of isovitexin, isoorientin, and isoscoparin; (2) proved the workflow's capability to discriminate within genotypes; (3) highlighted the role of glycosylation in barley phenylpropanoid diversity. Using the barley S42IL mapping population, the workflow proved useful for metabolic quantitative trait loci purposes. The protocol can be readily applied not only to explore the barley phenylpropanoid diversity represented in genebanks but also to study species whose profiles differ from those of cereals: the crop Helianthus annuus (sunflower) and the model plant Arabidopsis thaliana.

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Light and CO2 Modulate the Accumulation and Localization of Phenolic Compounds in Barley Leaves

Cited by 15 publications

References 71 publications

Barley Genotypes Vary in Stomatal Responsiveness to Light and CO2 Conditions

Barley Genotypes Vary in Stomatal Responsiveness to Light and CO2 Conditions

Regulation of Phenolic Compound Production by Light Varying in Spectral Quality and Total Irradiance

Untargeted metabotyping to study phenylpropanoid diversity in crop plants

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