Apomorphine Targets the Pleiotropic Bacterial Regulator Hfq

Turbant, Florian; Partouche, David; Hamoui, Omar El; Legoubey, Théa; Wien, Frank; Arluison, Véronique

doi:10.3390/antibiotics10030257

Cited by 8 publications

(3 citation statements)

References 58 publications

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“…Note that the use of larger liposomes could result in light scattering and for this reason, we used liposomes of 100 nm. For polymerized Hfq-CTR, the amyloid signature is found at ~220 nm ( Figure 3 ), as expected [ 25 , 49 , 50 ]. Then we added liposomes (EPE) and observed that the amyloid signature disappeared.…”

Section: Resultssupporting

confidence: 82%

Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator Hfq

Turbant

Waeytens

Campidelli

et al. 2022

IJMS

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Hfq is a pleiotropic regulator that mediates several aspects of bacterial RNA metabolism. The protein notably regulates translation efficiency and RNA decay in Gram-negative bacteria, usually via its interaction with small regulatory RNAs. Previously, we showed that the Hfq C-terminal region forms an amyloid-like structure and that these fibrils interact with membranes. The immediate consequence of this interaction is a disruption of the membrane, but the effect on Hfq structure was unknown. To investigate details of the mechanism of interaction, the present work uses different in vitro biophysical approaches. We show that the Hfq C-terminal region influences membrane integrity and, conversely, that the membrane specifically affects the amyloid assembly. The reported effect of this bacterial master regulator on membrane integrity is discussed in light of the possible consequence on small regulatory RNA-based regulation.

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Section: Resultssupporting

confidence: 82%

Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator Hfq

Turbant

Waeytens

Campidelli

et al. 2022

IJMS

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“…These reasons may explain a possible low level of heliotrine in the whole extract, and thus, a decreased potency against Gram-negative bacteria in the present study. Apomorphine was found to be bactericidal against E. coli by targeting the pleiotropic bacterial regulator Hfq [ 21 ]. Pinoresinol showed good antibacterial activities against most of bacteria studied in the current work [ 22 ], while propiconazole and mebendazole demonstrated their potency against several fungi strains including Cryptococcus neoformans and Sclerotium rolfsii [ 23, 24 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolomic profiling and bactericidal effect of Polyalthia longifolia (Sonn.) Twaites. stem bark against methicillin-resistant Staphylococcus aureus

Siwe

Simo

Maharjan

et al. 2023

Access Microbiology

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Objective. The present study was carried out to establish the chemical profile of the methanolic extract of Polyalthia longifolia stem bark and investigate its antibacterial property against some human pathogenic bacteria. Methods. The extract was analysed using liquid and gas chromatography coupled to mass spectrometry. Antibacterial activity of P. longifolia extract against some human pathogenic bacteria was screened using the AlamarBlue method, and MIC and MBC were determined. Results and Conclusion. Liquid chromatography-mass spectrometry (LC-MS) revealed the presence of 21 compounds among which 12 were identified. Gas chromatography-mass spectrometry (GC-MS) allowed identification of 26 compounds, the three major ones being the following: cis vaccenic acid (17.79 %), 3-ethyl-3-hydroxyandrostan-17-one (13.80 %) and copaiferic acid B (12.82 %). P. longifolia extract was active against Gram-positive bacteria with MIC ranging from 1 to 2 mg ml−1 and MBC from 2 to 6 mg ml−1. This study demonstrated the bactericidal effect of the methanolic extract of Polyalthia longifolia stem bark against some human pathogenic bacteria, including methicillin-resistant S. aureus . This effect could be related to the presence in the extract of a broad diversity of well-known compounds with established pharmacological properties. These results support the ethnomedicinal use of P. longifolia stem bark in Cameroon for the management of methicillin-resistant S. aureus (MRSA)-related infections.

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“…Due to the diversity of their targets, sRNAs and Hfq are involved in numerous bacterial pathways, including virulence and pathogenicity [ 15 , 16 ]. For this reason, Hfq has attracted considerable attention [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Role of the Bacterial Amyloid-like Hfq in Fluoroquinolone Fluxes

Turbant,

Esnouf,

Rosaz

et al. 2023

Microorganisms

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Due to their two-cell membranes, Gram-negative bacteria are particularly resistant to antibiotics. Recent investigations aimed at exploring new target proteins involved in Gram-negative bacteria adaptation helped to identify environmental changes encountered during infection. One of the most promising approaches in finding novel targets for antibacterial drugs consists of blocking noncoding RNA-based regulation using the protein cofactor, Hfq. Although Hfq is important in many bacterial pathogens, its involvement in antibiotics response is still unclear. Indeed, Hfq may mediate drug resistance by regulating the major efflux system in Escherichia coli, but it could also play a role in the influx of antibiotics. Here, using an imaging approach, we addressed this problem quantitatively at the single-cell level. More precisely, we analyzed how Hfq affects the dynamic influx and efflux of ciprofloxacin, an antibiotic from the group of fluoroquinolones that is used to treat bacterial infections. Our results indicated that the absence of either whole Hfq or its C-terminal domain resulted in a more effective accumulation of ciprofloxacin, irrespective of the presence of the functional AcrAB-TolC efflux pump. However, overproduction of the MicF small regulatory RNA, which reduces the efficiency of expression of the ompF gene (coding for a porin involved in antibiotics influx) in a Hfq-dependent manner, resulted in impaired accumulation of ciprofloxacin. These results led us to propose potential mechanisms of action of Hfq in the regulation of fluoroquinolone fluxes across the E. coli envelope.

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Apomorphine Targets the Pleiotropic Bacterial Regulator Hfq

Cited by 8 publications

References 58 publications

Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator Hfq

Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator Hfq

Metabolomic profiling and bactericidal effect of Polyalthia longifolia (Sonn.) Twaites. stem bark against methicillin-resistant Staphylococcus aureus

Role of the Bacterial Amyloid-like Hfq in Fluoroquinolone Fluxes

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