Biochemical Characterization of Recombinant UDPG-Dependent IAA Glucosyltransferase from Maize (Zea mays)

Ciarkowska, Anna; Ostrowski, Maciej; Kozakiewicz, Anna

doi:10.3390/ijms22073355

Cited by 2 publications

(1 citation statement)

References 47 publications

(109 reference statements)

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“…Lack of increase in IAGlc synthase activity may be due to the expression regulation on the translational level. It is also possible that other mechanisms of enzyme regulation are employed, such as regulation by post-translational modifications, as potential sites of N -glycosylation and phosphorylation are present in the protein sequence (Szerszen et al 1994 ), or by activity modulators such as ATP or glucose-1-phosphate that affect IAGlc synthase activity in concentration dependent manner (Ciarkowska et al 2021 ). As for IAInos synthase, we suppose that in 6-d-old germinating seeds transcription of ZmIAIn gets completely diminished to inactivate the second step of IAInos biosynthesis for IAA level to remain elevated.…”

Section: Discussionmentioning

confidence: 99%

Auxin homeostasis in maize (Zea mays) is regulated via 1-O-indole-3-acetyl-myo-inositol synthesis at early stages of seedling development and under abiotic stress

Ciarkowska

Wojtaczka

Kęsy

et al. 2022

Planta

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Main conclusion Indole-3-acetyl-myo-inositol biosynthesis is regulated during maize seedling development and in response to drought and cold stress. The main purpose of this pathway is maintenance of auxin homeostasis. Abstract Indole-3-acetic acid (IAA) conjugation to myo-inositol is a part of a mechanism controlling free auxin level in maize. In this work, we investigated changes in the indole-3-acetyl-myo-inositol (IAInos) biosynthesis pathway in 3-d- and 6-d-old maize seedlings and germinating seeds as well as in seedlings subjected to drought and cold stress to evaluate a role of this pathway in maize development and stress response. In germinating seeds, activity of the enzymes involved in IAInos biosynthesis remains unchanged between 3-d- and 6-d-old material but increases in coleoptiles and radicles of the seedlings. Under cold stress, in germinating seeds and in coleoptiles, activity of the enzymes decreases and increases, respectively; however, it does not entail changes in auxin level. In drought-exposed germinating maize seeds, totally diminished activities of IAInos synthesis pathway enzymes resulted in almost twofold increase of free IAA content. Similar increase of auxin level was observed in radicles of drought-subjected seedlings together with lack of catalytic activity of the first enzyme of the pathway. Exogenous IAInos has no effect on the level of non-enzymatic antioxidant, ascorbate. It has also either no effect on the protein carbonylation and lipid peroxidation, or it affects it in a similar way as exogenously applied IAA and myo-inositol, which are products of IAInos hydrolysis. Thus, IAInos biosynthesis pathway acts in maize development and stress responses by regulation of free IAA concentration, as IAInos itself does not appear to have a distinct role in these processes.

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Section: Discussionmentioning

confidence: 99%

Auxin homeostasis in maize (Zea mays) is regulated via 1-O-indole-3-acetyl-myo-inositol synthesis at early stages of seedling development and under abiotic stress

Ciarkowska

Wojtaczka

Kęsy

et al. 2022

Planta

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Horticultural potential of chemical biology to improve adventitious rooting

Pincelli-Souza,

Tang,

Miller

et al. 2024

HORTIC. ADV.

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The first reports that auxins promoted root formation in cuttings and that indole-3-butyric acid (IBA) was a particularly effective treatment date from the early 1930s. Since its introduction into horticultural practice, the focus on improvements in the rooting of plants has been largely on the proper use of auxins to enhance adventitious rooting (AR) as well as to increase the range of plants where it can be effective. In this review, we focus on new ideas that might build on what is known about auxin induction of AR. We explore what the evolution in chemical biology has opened through novel high-throughput screening tools to explore auxin regulation of plant development and what it might add to our understanding and potential to produce new tools for the manipulation of AR. The potential for using stronger auxin analogues, alternative indolealkanoic acids, compounds that alter β-oxidation of IBA and other indolealkanoic acids, auxin conjugates, inhibitors of auxin conjugation, inhibitors of endogenous auxin biosynthesis, as well as other plant hormones and compounds that inhibit the production or mimic the effects of signals that might be involved in AR are all discussed. The expectation is that a summary of these advances in our understanding of the chemical biology important to AR might increase the use and exploration of new ideas for the improvement in the practical approaches to advance horticultural rooting methods.

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Biochemical Characterization of Recombinant UDPG-Dependent IAA Glucosyltransferase from Maize (Zea mays)

Cited by 2 publications

References 47 publications

Auxin homeostasis in maize (Zea mays) is regulated via 1-O-indole-3-acetyl-myo-inositol synthesis at early stages of seedling development and under abiotic stress

Auxin homeostasis in maize (Zea mays) is regulated via 1-O-indole-3-acetyl-myo-inositol synthesis at early stages of seedling development and under abiotic stress

Horticultural potential of chemical biology to improve adventitious rooting

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