Magnesium Influences Membrane Fusion during Myogenesis by Modulating Oxidative Stress in C2C12 Myoblasts

Zocchi, Monica; Béchet, Daniel; Mazur, Andrzej; Maier, Jeanette A. M.; Castiglioni, Sara

doi:10.3390/nu13041049

Cited by 8 publications

(7 citation statements)

References 26 publications

(32 reference statements)

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“…In the muscle of hypomagnesemic mice, a significant downregulation of Mg transporters has been observed. Moreover, we demonstrated that Mg is required to accomplish myogenic differentiation and membrane fusion since non-physiological extracellular Mg concentrations impact the myoblasts ability to fuse and form multinucleated cells [6]. Nonetheless, very little remains known about Mg homeostasis and its transporters in myogenesis.…”

Section: Discussionmentioning

confidence: 98%

“…It has been proposed that Mg might play a role in the myogenic process since Mg deficiency enhances the expression of the myogenic proteins Myogenic Differentiation 1 (MyoD) and Myogenin (Myog) in rat skeletal muscle [5]. Moreover, we have recently demonstrated that non-physiological low or high extracellular Mg concentrations inhibit myogenesis in C2C12 myoblasts, through an ROS-mediated impairment of membrane fusion [6].…”

Section: Introductionmentioning

confidence: 94%

“…C2C12 were induced to differentiate using a differentiation medium (DM) containing 2% horse serum, a process that requires 144 h under our experimental conditions [6]. Cell differentiation was monitored by optical microscopy.…”

Section: Mg Concentration Decreases During Myogenesis and Is Restored In Myotubesmentioning

confidence: 99%

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Magnesium Homeostasis in Myogenic Differentiation—A Focus on the Regulation of TRPM7, MagT1 and SLC41A1 Transporters

Zocchi

Locatelli

Zuccotti

et al. 2022

IJMS

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Magnesium (Mg) is essential for skeletal muscle health, but little is known about the modulation of Mg and its transporters in myogenic differentiation. Here, we show in C2C12 murine myoblasts that Mg concentration fluctuates during their differentiation to myotubes, declining early in the process and reverting to basal levels once the cells are differentiated. The level of the Mg transporter MagT1 decreases at early time points and is restored at the end of the process, suggesting a possible role in the regulation of intracellular Mg concentration. In contrast, TRPM7 is rapidly downregulated and remains undetectable in myotubes. The reduced amounts of TRPM7 and MagT1 are due to autophagy, one of the proteolytic systems activated during myogenesis and essential for the membrane fusion process. Moreover, we investigated the levels of SLC41A1, which increase once cells are differentiated, mainly through transcriptional regulation. In conclusion, myogenesis is associated with alterations of Mg homeostasis finely tuned through the modulation of MagT1, TRPM7 and SLC41A1.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 94%

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Magnesium Homeostasis in Myogenic Differentiation—A Focus on the Regulation of TRPM7, MagT1 and SLC41A1 Transporters

Zocchi

Locatelli

Zuccotti

et al. 2022

IJMS

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“…This is not surprising since magnesium is key for all the enzymes utilizing or synthesizing muscle ATP, and thus for the production of muscle energy, and also regulates contraction and relaxation [ 25 ]. Additionally, magnesium assures the regenerative capacity of skeletal muscle fibers [ 91 ].…”

Section: Introductionmentioning

confidence: 99%

The relevance of magnesium homeostasis in COVID-19

Trapani

Rosanoff²,

Baniasadi

et al. 2021

Eur J Nutr

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Purpose In less than one and a half year, the COVID-19 pandemic has nearly brought to a collapse our health care and economic systems. The scientific research community has concentrated all possible efforts to understand the pathogenesis of this complex disease, and several groups have recently emphasized recommendations for nutritional support in COVID-19 patients. In this scoping review, we aim at encouraging a deeper appreciation of magnesium in clinical nutrition, in view of the vital role of magnesium and the numerous links between the pathophysiology of SARS-CoV-2 infection and magnesium-dependent functions. Methods By searching PubMed and Google Scholar from 1990 to date, we review existing evidence from experimental and clinical studies on the role of magnesium in chronic non-communicable diseases and infectious diseases, and we focus on recent reports of alterations of magnesium homeostasis in COVID-19 patients and their association with disease outcomes. Importantly, we conduct a census on ongoing clinical trials specifically dedicated to disclosing the role of magnesium in COVID-19. Results Despite many methodological limitations, existing data seem to corroborate an association between deranged magnesium homeostasis and COVID-19, and call for further and better studies to explore the prophylactic or therapeutic potential of magnesium supplementation. Conclusion We propose to reconsider the relevance of magnesium, frequently overlooked in clinical practice. Therefore, magnesemia should be monitored and, in case of imbalanced magnesium homeostasis, an appropriate nutritional regimen or supplementation might contribute to protect against SARS-CoV-2 infection, reduce severity of COVID-19 symptoms and facilitate the recovery after the acute phase.

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“…Magnesium also has a fundamental role during myogenesis. Non-physiological magnesium concentrations induce oxidative stress in myoblasts, and this oxidative stress is responsible for the inhibition of myoblast membrane fusion, thus impairing myogenesis [ 11 ]. Magnesium also affects bone growth and remodelling.…”

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confidence: 99%