The Banana Root Endophytome: Differences between Mother Plants and Suckers and Evaluation of Selected Bacteria to Control Fusarium oxysporum f.sp. cubense

Background: Natural plant extracts and microbial antagonists have the potential for use in increasing the fungal resistance and productivity of horticulture plants. Methods: The purpose of this study was to evaluate the ability of both natural plant extracts and microbial antagonists as a biotical control of some fungal pathogens, i.e., Fusarium ssp., Exserohilum ssp. and Nigrospora ssp., along with improving the growth and productivity performance of zucchini under greenhouse conditions. Eucalyptus camaldulensis leaf extract (LE), Citrus sinensis LE, Ficus benghalensis fruit extract (FE), and two microbial antagonists Pseudomonas fluorescens (accession no. MW647093) and Trichoderma viride (accession no. MW647090) were tested under in vitro and in vivo conditions. Through morphological characteristics and the internal transcribed spacer (ITS) region, Fusarium solani (accession no. MW947256), F. oxysporum (accession no. MW947254), Exserohilum rostratum (accession no. MW947255), and Nigrospora lacticolonia (accession no. MW947253) were identified. HPLC analysis was used for the identification of phenolic compounds (PCs) and flavonoid compounds (FCs) in the extracts. Results: The highest inhibition percentage of fungal growth (IPFG) against F. oxysporum was obtained with P. fluorescens, T. viride, and E. camaldulensis LE (4000 mg/L); F. solani with P. fluorescens, T. viride, and C. sinensis LE (4000 mg/L); Exserohilum rostratum with P. fluorescens, Ficus benghalensis FE (4000 mg/L) and E. camaldulensis LE (4000 mg/L), and N. lacticolonia with P. fluorescens. Using HPLC analysis, the abundant PCs in E. camaldulensis LE were pyrogallol, and caffeic acid, those in C. sinensis LE were syringic acid and ferulic acid, and those in F. benghalensis FE were gallic acid and syringic acid. In addition, the abundant FCs in E. camaldulensis LE were kaempferol, and naringin, those in C. sinensis LE were hesperidin and quercetin, and those in F. benghalensis FE were kaempferol and quercetin. Under greenhouse experiments, T. viride and E. camaldulensis LE (4000 mg/L) followed by P. fluorescens + T. viride treatments gave the best results of zucchini plants in terms of leaf area, fruits number per plant, yield per plant, and total yield (marketable and non-marketable). Conclusions: Plant extracts and bioagents can be used to control some zucchini fungal pathogens and increase the productivity performance of zucchini plants.

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“…In addition, potential biocontrol agents against F. oxysporum f.sp. cubense (Foc) races STR4 and TR4 on banana plants was achieved [82].…”

Section: Discussionmentioning

confidence: 99%

Natural Plant Extracts and Microbial Antagonists to Control Fungal Pathogens and Improve the Productivity of Zucchini (Cucurbita pepo L.) In Vitro and in Greenhouse

Hassan

Mohamed²,

Feleafel

et al. 2021

Horticulturae

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“…Among them, Pseudomonas simiae PICF7 [ 33 , 35 ] stands out as a well-known and versatile BCA and/or PGP, not only in its natural host (olive) but also in distant plant species such as Arabidopsis thaliana , barley ( Hordeum vulgare L.) and banana ( Musa acuminata L. AAA group, cv. Cavendish) [ 27 , 36 , 37 , 38 ]. In contrast, the available information for another beneficial olive-derived rhizobacterium, Pseudomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Identification of Volatile Organic Compounds Emitted by Two Beneficial Endophytic Pseudomonas Strains from Olive Roots

Montes-Osuna

Cernava

Cabanás

et al. 2022

Plants

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The production of volatile organic compounds (VOCs) represents a promising strategy of plant-beneficial bacteria to control soil-borne phytopathogens. Pseudomonas sp. PICF6 and Pseudomonas simiae PICF7 are two indigenous inhabitants of olive roots displaying effective biological control against Verticillium dahliae. Additionally, strain PICF7 is able to promote the growth of barley and Arabidopsis thaliana, VOCs being involved in the growth of the latter species. In this study, the antagonistic capacity of these endophytic bacteria against relevant phytopathogens (Verticillium spp., Rhizoctonia solani, Sclerotinia sclerotiorum and Fusarium oxysporum f.sp. lycopersici) was assessed. Under in vitro conditions, PICF6 and PICF7 were only able to antagonize representative isolates of V. dahliae and V. longisporum. Remarkably, both strains produced an impressive portfolio of up to twenty VOCs, that included compounds with reported antifungal (e.g., 1-undecene, (methyldisulfanyl) methane and 1-decene) or plant growth promoting (e.g., tridecane, 1-decene) activities. Moreover, their volatilomes differed strongly in the absence and presence of V. dahliae. For example, when co incubated with the defoliating pathotype of V. dahliae, the antifungal compound 4-methyl-2,6-bis(2-methyl-2-propanyl)phenol was produced. Results suggest that volatiles emitted by these endophytes may differ in their modes of action, and that potential benefits for the host needs further investigation in planta.

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“…This strain is phylogenetically close to P. simiae (originally designated P. fluorescens) WCS417r (Gómez-Lama Cabánas et al 2018;Montes-Osuna et al 2021) and presents the following features: (i) a sequenced genome (Martínez-García et al 2015); (ii) an endophytic lifestyle in olive roots and cultivated cereals, but it only colonizes the surface of Arabidopsis roots Mercado-Blanco et al 2016); and (iii) biocontrol activity against Verticillium dahliae in olive trees (Montes-Osuna et al 2021), Botrytis cinerea in Arabidopsis , and Fusarium oxysporum f. sp. cubense in banana (Gómez-Lama Cabánas et al 2021).…”

Section: Bacterial Strainsmentioning

confidence: 99%

Transcriptional regulation of plant sugar transporter genes by beneficial rhizobacteria

Desrut

Thibault

Mercado‐Blanco

et al. 2021

Journal of Plant Interactions

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In their natural environment, plants live in close interaction with complex populations of microorganisms, including rhizobacteria species commonly referred to as 'Plant Growth Promoting Rhizobacteria' (PGPR). A growing body of evidence demonstrates the importance of sugar transport in plant pathogen resistance and in plant-microorganism mutualistic symbioses. Using an in vitro experimental system, including the model plant species Arabidopsis thaliana, two PGPR strains (Pseudomonas simiae PICF7 and Burkholderia phytofirmans PsJN) and a non-PGPR strain (Escherichia coli), we conducted a comprehensive set of phenotypic and gene expression analyses to explore the role and regulation of sugar transporter genes in plant-PGPR interactions. In physical contact with the seedling roots, or solely via the emission of bacterial volatile compounds, the two PGPR strains tested improved the growth and development of the Arabidopsis seedlings and altered the expression of several plant sugar transporter genes. Our results also revealed both conserved and strain-specific transcriptional regulation mechanisms.

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The Banana Root Endophytome: Differences between Mother Plants and Suckers and Evaluation of Selected Bacteria to Control Fusarium oxysporum f.sp. cubense

Cited by 39 publications

References 84 publications

Natural Plant Extracts and Microbial Antagonists to Control Fungal Pathogens and Improve the Productivity of Zucchini (Cucurbita pepo L.) In Vitro and in Greenhouse

Natural Plant Extracts and Microbial Antagonists to Control Fungal Pathogens and Improve the Productivity of Zucchini (Cucurbita pepo L.) In Vitro and in Greenhouse

Identification of Volatile Organic Compounds Emitted by Two Beneficial Endophytic Pseudomonas Strains from Olive Roots

Transcriptional regulation of plant sugar transporter genes by beneficial rhizobacteria

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