An Alternative Splicing Variant of PtRD26 Delays Leaf Senescence by Regulating Multiple NAC Transcription Factors in <i>Populus</i>

Wang, Hou‐Ling; Zhang, Yi; Wang, Ting; Yang, Qi; Yang, Yanli; Li, Ze; Li, Bosheng; Xing, Wen; Li, Wenyang; Yin, Weilun; Xia, Xinli; Guo, Hongwei; Li, Zhonghai

doi:10.1093/plcell/koab046

Cited by 87 publications

(72 citation statements)

References 88 publications

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“…The specific primers were designed by Primer Premier 6.0 and examined by NCBI Primer-BLAST (https://www.ncbi.nlm.nih.gov/ tools/primerblast/, accessed on 15 July 2021) (Supplementary Table S4). The selection of reference genes of P. euphratica was based on the research of Wang et al [80,81]. Real-time PCR was run with the CFX96 Touch™ instrument (Bio-Rad, Hercules, CA, USA) to detect the chemical SYBR Green.…”

Section: Rna Extraction Cdna Synthesis and Qrt-pcr Analysismentioning

confidence: 99%

Genome-Wide Comprehensive Analysis of the GASA Gene Family in Populus

Han

Jiao

Niu

et al. 2021

IJMS

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Gibberellic acid-stimulated Arabidopsis (GASA) proteins, as cysteine-rich peptides (CRPs), play roles in development and reproduction and biotic and abiotic stresses. Although the GASA gene family has been identified in plants, the knowledge about GASAs in Populus euphratica, the woody model plant for studying abiotic stress, remains limited. Here, we referenced the well-sequenced Populus trichocarpa genome, and identified the GASAs in the whole genome of P. euphratica and P. trichocarpa. 21 candidate genes in P. trichocarpa and 19 candidate genes in P. euphratica were identified and categorized into three subfamilies by phylogenetic analysis. Most GASAs with signal peptides were located extracellularly. The GASA genes in Populus have experienced multiple gene duplication events, especially in the subfamily A. The evolution of the subfamily A, with the largest number of members, can be attributed to whole-genome duplication (WGD) and tandem duplication (TD). Collinearity analysis showed that WGD genes played a leading role in the evolution of GASA genes subfamily B. The expression patterns of P. trichocarpa and P. euphratica were investigated using the PlantGenIE database and the real-time quantitative PCR (qRT-PCR), respectively. GASA genes in P. trichocarpa and P. euphratica were mainly expressed in young tissues and organs, and almost rarely expressed in mature leaves. GASA genes in P. euphratica leaves were also widely involved in hormone responses and drought stress responses. GUS activity assay showed that PeuGASA15 was widely present in various organs of the plant, especially in vascular bundles, and was induced by auxin and inhibited by mannitol dramatically. In summary, this present study provides a theoretical foundation for further research on the function of GASA genes in P. euphratica.

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Section: Rna Extraction Cdna Synthesis and Qrt-pcr Analysismentioning

confidence: 99%

Genome-Wide Comprehensive Analysis of the GASA Gene Family in Populus

Han

Jiao

Niu

et al. 2021

IJMS

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“…Owing to their crucial function in the formation of the embryos and flowers of petunias, NAC TFs were widely studied, and multiple reports identified its key role in plant growth and development [12,13]. A large number of NAC-encoding genes have been identified and confirmed in various plants for their role in regulating wood formation [14], leaf senescence [15], fruit development [16,17], and flower morphogenesis [18,19]. Furthermore, NAC TFs also are involved in various biotic and abiotic stress responses in many plants such as Populus tomentosa [20], Arabidopsis thaliana [21], Oryza sativa [22,23], Glycine max [24], Arachis hypogaea [25], and Xanthoceras sorbifolia [26].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of NAC Transcription Factor Family in Juglans mandshurica and Their Expression Analysis during the Fruit Development and Ripening

Cai

Pei

et al. 2021

IJMS

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The NAC (NAM, ATAF and CUC) gene family plays a crucial role in the transcriptional regulation of various biological processes and has been identified and characterized in multiple plant species. However, genome-wide identification of this gene family has not been implemented in Juglans mandshurica, and specific functions of these genes in the development of fruits remain unknown. In this study, we performed genome-wide identification and functional analysis of the NAC gene family during fruit development and identified a total of 114 JmNAC genes in the J. mandshurica genome. Chromosomal location analysis revealed that JmNAC genes were unevenly distributed in 16 chromosomes; the highest numbers were found in chromosomes 2 and 4. Furthermore, according to the homologues of JmNAC genes in Arabidopsis thaliana, a phylogenetic tree was constructed, and the results demonstrated 114 JmNAC genes, which were divided into eight subgroups. Four JmNAC gene pairs were identified as the result of tandem duplicates. Tissue-specific analysis of JmNAC genes during different developmental stages revealed that 39 and 25 JmNAC genes exhibited upregulation during the mature stage in walnut exocarp and embryos, indicating that they may serve key functions in fruit development. Furthermore, 12 upregulated JmNAC genes were common in fruit ripening stage in walnut exocarp and embryos, which demonstrated that these genes were positively correlated with fruit development in J. mandshurica. This study provides new insights into the regulatory functions of JmNAC genes during fruit development in J. mandshurica, thereby improving the understanding of characteristics and evolution of the JmNAC gene family.

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“…A large number of studies have shown that TF plays a key regulatory role in leaf senescence, however, most of the studies have mainly focused on a few families, including the NAC or WRKY families, and more studies are needed in the future to analyze whether other family TFs are also involved in leaf senescence. A recent transcriptomic study revealed that 115 Sen-TFs from 31 families are involved in autumn leaf senescence in poplar ( Wang et al, 2021 ), further supporting this suggestion.…”

Section: Multiple-layers Of Regulation On Leaf Senescencementioning

confidence: 62%

Multiple Layers of Regulation on Leaf Senescence: New Advances and Perspectives

et al. 2021

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Leaf senescence is the last stage of leaf development and is an orderly biological process accompanied by degradation of macromolecules and nutrient recycling, which contributes to plant fitness. Forward genetic mutant screening and reverse genetic studies of senescence-associated genes (SAGs) have revealed that leaf senescence is a genetically regulated process, and the initiation and progression of leaf senescence are influenced by an array of internal and external factors. Recently, multi-omics techniques have revealed that leaf senescence is subjected to multiple layers of regulation, including chromatin, transcriptional and post-transcriptional, as well as translational and post-translational levels. Although impressive progress has been made in plant senescence research, especially the identification and functional analysis of a large number of SAGs in crop plants, we still have not unraveled the mystery of plant senescence, and there are some urgent scientific questions in this field, such as when plant senescence is initiated and how senescence signals are transmitted. This paper reviews recent advances in the multiple layers of regulation on leaf senescence, especially in post-transcriptional regulation such as alternative splicing.

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An Alternative Splicing Variant of PtRD26 Delays Leaf Senescence by Regulating Multiple NAC Transcription Factors in Populus

Cited by 87 publications

References 88 publications

Genome-Wide Comprehensive Analysis of the GASA Gene Family in Populus

Genome-Wide Comprehensive Analysis of the GASA Gene Family in Populus

Genome-Wide Identification of NAC Transcription Factor Family in Juglans mandshurica and Their Expression Analysis during the Fruit Development and Ripening

Multiple Layers of Regulation on Leaf Senescence: New Advances and Perspectives

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