Stability and validity of intact parathyroid hormone levels in different sample types and storage conditions

Khalil, Haitham; Borai, Anwar; Dakhakhni, Mohammed; Bahijri, Suhad; Faizo, Hala; Bokhari, Fawzi; Ferns, Gordon A.; Mirza, Ahmed A.

doi:10.1002/jcla.23771

Cited by 4 publications

(2 citation statements)

References 16 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An interesting finding in the study is that boys with components of biochemical osteomalacia were significantly taller than their counterparts without biochemical osteomalacia. This may well correspond to some of the boys still undergoing the pubertal growth spurt when bone growth outpaces bone mineralization, which may potentially lead to a relative reduction in bone mineral content [34].…”

Section: Discussionmentioning

confidence: 86%

Establishing the Prevalence of Osteomalacia in Arab Adolescents Using Biochemical Markers of Bone Health

et al. 2022

View full text Add to dashboard Cite

Nutrition-acquired osteomalacia is a bone mineralization disorder associated with dietary calcium and/or solar vitamin D deficiency, risk factors considered common in the Middle Eastern region. Establishing less invasive, cheap, and widely available diagnostic markers for this underdiagnosed entity is essential, in particular for screening in high-risk groups. This study assessed the prevalence of biochemical osteomalacia in Arab adolescents. In this cross-sectional study performed between September 2019 and March 2021, adolescents aged 12–17 years from 60 different secondary and preparatory year schools in Riyadh, Saudi Arabia were included. Anthropometrics and fasting blood samples were collected. Biochemical osteomalacia was defined as any two of the following four serum markers of hypomineralization, namely low 25 hydroxyvitamin D (25OHD < 30 nmol/L), high alkaline phosphatase (ALP), low calcium (Ca), and/or inorganic phosphorous (Pi). A total of 2938 Arab adolescents [1697 girls; mean age (years) 14.8 ± 1.8; 1241 boys; mean age 15.1 ± 1.6] were recruited. Vitamin D deficiency was noted in 56.2% (n = 953) of girls and 27.1% (n = 336) of boys (p < 0.001). The overall prevalence of biochemical osteomalacia was 10.0% (n = 295/2938) and was higher in girls than boys (14.7% vs. 3.6%, p < 0.001). The prevalence of low serum Ca and/or Pi was also higher in girls than in boys (24.2% vs. 12.5%, respectively, p < 0.001), as well as elevated ALP (5.1% vs. 1.5%, p < 0.001). Overall, girls were 4.6 times (95% CI 3.3–6.4) more likely to have biochemical osteomalacia than boys. Screening of apparently healthy Arab adolescents revealed a high prevalence of deranged mineralization markers suggestive of biochemical osteomalacia, which was significantly more common in girls than boys and was likely associated with Arab traditional clothing and diet. The proposed combination of typically altered mineralization markers for the diagnosis of osteomalacia is, at best, suggestive until further comparisons with established diagnostic tools (histological analysis of bone biopsies) are conducted.

show abstract

Section: Discussionmentioning

confidence: 86%

Establishing the Prevalence of Osteomalacia in Arab Adolescents Using Biochemical Markers of Bone Health

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In addition to assay-related differences in age-related PTH reference intervals, there may be geographical differences in reference intervals because of differences in population (for example, ethnicity, age and sex distribution, deprivation, prevalence of negative calcium balance, proton pump inhibitor use, calcium and vitamin D supplementation practices), laboratory practices and method used to drive reference intervals as was found in a recent Abbott PTH reference interval study comparing 4 different UK sites [ 38 ]. PTH is more stable in EDTA plasma than in serum at room temperature [ 39 ]. Therefore, age-specific iPTH reference intervals may be slightly higher in EDTA plasma than in serum.…”

Section: Discussionmentioning

confidence: 99%

Age-specific Reference Intervals of Abbott Intact PTH—Potential Impacts on Clinical Care

Kalaria,

Lawson,

Duffy

et al. 2024

Journal of the Endocrine Society

View full text Add to dashboard Cite

Background Parathyroid hormone (PTH) assays are not standardized. Therefore, method-specific PTH reference intervals are required for interpretation of results. PTH increases with age in adults but age-related reference intervals for the Abbott intact PTH (iPTH) assay are not available. Methods Deidentified serum PTH results from September 2015 to November 2022 were retrieved from the laboratory information system of a laboratory serving a cosmopolitan population in central-west England for individuals aged 18 years and older if the estimated glomerular filtration rate (eGFR) was ≥ 60 mL/min, serum 25-hydroxyvitamin D was > 50 nmol/L, and serum albumin adjusted calcium and serum phosphate were within reference intervals. Age-specific reference intervals for Abbott iPTH were derived by an indirect method using the refineR algorithm. Results PTH increased with age and correlated with age when controlled for 25-hydroxyvitamin D, eGFR and adjusted calcium (r = 0.093, p < 0.001). The iPTH age-specific reference intervals for four age partitions of 18 to 45 years, 46 to 60 years, 61 to 80 years, and 81 to 95 years were 1.6–8.6 pmol/L, 1.8–9.5 pmol/L, 2.0–11.3 pmol/L and 2.3–12.3 pmol/L, respectively. PTH was higher in women compared to men (p < 0.001). Sex-specific age-related reference intervals could not be derived because of the limited sample size. Conclusions Age-specific Abbott iPTH reference intervals were derived. Application of age-specific reference intervals will impact the diagnosis and management of normocalcemic hyperparathyroidism, based on current definitions, and secondary hyperparathyroidism. Additional studies are required to clarify the effect of sex and ethnicity on PTH.

show abstract

Preanalytical considerations in parathyroid hormone measurement

Cheng

Wang

et al. 2023

Clinica Chimica Acta

View full text Add to dashboard Cite

Stability and validity of intact parathyroid hormone levels in different sample types and storage conditions

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 4 publications

References 16 publications

Establishing the Prevalence of Osteomalacia in Arab Adolescents Using Biochemical Markers of Bone Health

Establishing the Prevalence of Osteomalacia in Arab Adolescents Using Biochemical Markers of Bone Health

Age-specific Reference Intervals of Abbott Intact PTH—Potential Impacts on Clinical Care

Preanalytical considerations in parathyroid hormone measurement

Contact Info

Product

Resources

About