X-ray absorption spectroscopy evidence of sulfur-bound cadmium in the Cd-hyperaccumulator Solanum nigrum and the non-accumulator Solanum melongena

Pons, Marie-Laure; Collin, Blanche; Dœlsch, Emmanuel; Chaurand, Perrine; Fehlauer, Till; Levard, Clément; Keller, Catherine; Rose, Jérôme

doi:10.1016/j.envpol.2021.116897

Cited by 15 publications

(3 citation statements)

References 77 publications

(132 reference statements)

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“…The health and environmental implications of cadmium exposure have, nonetheless, received increasing attention, particularly given the frequent deployment of cadmium in applications such as battery production, the plastics and coating sectors, and metal smelting [ 4 , 5 ]. In the soil of mainland China, concentrations of cadmium vary widely, from 0.003 mg/kg to 9.57 mg/kg [ 6 ]. Within fresh water, cadmium residues are normally detected at levels of 10 to 500 ng/L, but this contrasts starkly with the highest level observed in industrialized regions, in excess of 17 mg/L [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Cadmium-induced apoptosis of Leydig cells is mediated by excessive mitochondrial fission and inhibition of mitophagy

Shang

et al. 2022

Cell Death Dis

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Cadmium is one of the environmental and occupational pollutants and its potential adverse effects on human health have given rise to substantial concern. Cadmium causes damage to the male reproductive system via induction of germ-cell apoptosis; however, the underlying mechanism of cadmium-induced reproductive toxicity in Leydig cells remains unclear. In this study, twenty mice were divided randomly into four groups and exposed to CdCl2 at concentrations of 0, 0.5, 1.0 and 2.0 mg/kg/day for four consecutive weeks. Testicular injury, abnormal spermatogenesis and apoptosis of Leydig cells were observed in mice. In order to investigate the mechanism of cadmium-induced apoptosis of Leydig cells, a model of mouse Leydig cell line (i.e. TM3 cells) was subjected to treatment with various concentrations of CdCl2. It was found that mitochondrial function was disrupted by cadmium, which also caused a significant elevation in levels of mitochondrial superoxide and cellular ROS. Furthermore, while cadmium increased the expression of mitochondrial fission proteins (DRP1 and FIS1), it reduced the expression of mitochondrial fusion proteins (OPA1 and MFN1). This led to excessive mitochondrial fission, the release of cytochrome c and apoptosis. Conversely, cadmium-induced accumulation of mitochondrial superoxide was decreased by the inhibition of mitochondrial fission through the use of Mdivi-1 (an inhibitor of DRP1). Mdivi-1 also partially prevented the release of cytochrome c from mitochondria to cytosol and attenuated cell apoptosis. Finally, given the accumulation of LC3II and SQSTM1/p62 and the obstruction of Parkin recruitment into damaged mitochondria in TM3 cells, the autophagosome-lysosome fusion was probably inhibited by cadmium. Overall, these findings suggest that cadmium induces apoptosis of mouse Leydig cells via the induction of excessive mitochondrial fission and inhibition of mitophagy.

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Section: Introductionmentioning

confidence: 99%

Cadmium-induced apoptosis of Leydig cells is mediated by excessive mitochondrial fission and inhibition of mitophagy

Shang

et al. 2022

Cell Death Dis

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“…Under Cd stress, hyperaccumulators demonstrate an exceptional ability to accumulate Cd in their tissues attributed to the evolution of detoxification mechanisms that mitigate its toxic effects. For instance, in the Cd hyperaccumulator Solanum nigrum , Cd binding to sulfur ligands serves as a detoxification mechanism, which likely involves the sequestration of Cd complexes with glutathione or phytochelatins in plant vacuoles leading to greater Cd accumulation than in the nonaccumulator Solanum melongena ( Pons et al., 2021 ). Moreover, the cell wall biosynthesis pathway has also been identified as another significant contributor to Cd detoxification in S. nigrum ( Wang et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Integrative physiological, transcriptomic, and metabolomic analysis of Abelmoschus manihot in response to Cd toxicity

Wu,

Xu,

Tang

et al. 2024

Front. Plant Sci.

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Rapid industrialization and urbanization have caused severe soil contamination with cadmium (Cd) necessitating effective remediation strategies. Phytoremediation is a widely adopted technology for remediating Cd-contaminated soil. Previous studies have shown that Abelmoschus manihot has a high Cd accumulation capacity and tolerance indicating its potential for Cd soil remediation. However, the mechanisms underlying its response to Cd stress remain unclear. In this study, physiological, transcriptomic, and metabolomic analyses were conducted to explore the response of A. manihot roots to Cd stress at different time points. The results revealed that Cd stress significantly increased malondialdehyde (MDA) levels in A. manihot, which simultaneously activated its antioxidant defense system, enhancing the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) by 19.73%–50%, 22.87%–38.89%, and 32.31%–45.40% at 12 h, 36 h, 72 h, and 7 days, respectively, compared with those in the control (CK). Moreover, transcriptomic and metabolomic analyses revealed 245, 5,708, 9,834, and 2,323 differentially expressed genes (DEGs), along with 66, 62, 156, and 90 differentially expressed metabolites (DEMs) at 12 h, 36 h, 72 h, and 7 days, respectively. Through weighted gene coexpression network analysis (WGCNA) of physiological indicators and transcript expression, eight hub genes involved in phenylpropanoid biosynthesis, signal transduction, and metal transport were identified. In addition, integrative analyses of metabolomic and transcriptomic data highlighted the activation of lipid metabolism and phenylpropanoid biosynthesis pathways under Cd stress suggesting that these pathways play crucial roles in the detoxification process and in enhancing Cd tolerance in A. manihot. This comprehensive study provides detailed insights into the response mechanisms of A. manihot to Cd toxicity.

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“…There are two types of application of XANES to plants. One focuses on the location and speciation of metals in hyperaccumulators (Pons et al, 2021;Matzen et al, 2022), and the other is dedicated to investigating HMs in food crops and vegetables (Jin et al, 2022;Kunene et al, 2021;Abbasi et al, 2022;Brier et al, 2016;Ogunkunle et al, 2019). For instance, element distribution and Pb speciation in vegetable and grain seeds during germination have been studied using XANES and XRF.…”

Section: Introductionmentioning

confidence: 99%

Pb speciation and elemental distribution in leeks by micro X-ray fluorescence and X-ray absorption near-edge structure

Sun,

Yang,

Luo

2023

J Synchrotron Radiat

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Vegetables are crucial to a human diet as they supply the body with essential vitamins, minerals, etc. Heavy metals that accumulate in plants consequently enter the food chain and endanger people's health. Studying the spatial distribution and chemical forms of elements in plant/vegetable tissues is vital to comprehending the potential interactions between elements and detoxification mechanisms. In this study, leek plants and soil from vegetable gardens near lead–zinc mines were collected and cultivated with 500 mg L−1 PbNO3 solutions for three weeks. Micro X-ray fluorescence was used to map the distribution of Pb and other chemical elements in leek roots, and X-ray absorption near-edge spectroscopy was used to assess the Pb speciation in leek roots and leaves. These findings demonstrated that Pb, Cu, Mn, Cr, Ti and Fe were detected in the outer rings of the root's cross section, and high-intensity points were observed in the epidermis. Zn, K and Ca, on the other hand, were distributed throughout the root's cross section. Leek root and leaf contained significant quantities of lead phosphate and basic lead carbonate at more than 80%, followed by lead sulfide (19%) and lead stearate (11.1%). The capacity of leek roots to convert ambient lead into precipitated lead and fix it on the root epidermis and other inner surfaces is a key mechanism for reducing the toxic effects of Pb.

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X-ray absorption spectroscopy evidence of sulfur-bound cadmium in the Cd-hyperaccumulator Solanum nigrum and the non-accumulator Solanum melongena

Cited by 15 publications

References 77 publications

Cadmium-induced apoptosis of Leydig cells is mediated by excessive mitochondrial fission and inhibition of mitophagy

Cadmium-induced apoptosis of Leydig cells is mediated by excessive mitochondrial fission and inhibition of mitophagy

Integrative physiological, transcriptomic, and metabolomic analysis of Abelmoschus manihot in response to Cd toxicity

Pb speciation and elemental distribution in leeks by micro X-ray fluorescence and X-ray absorption near-edge structure

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