CytoBas: Precision component‐resolved diagnostics for allergy using flow cytometric staining of basophils with recombinant allergen tetramers

McKenzie, Craig; Varese, Nirupama; Aui, Pei Mun; Wines, Bruce D.; Hogarth, P. M.; Thien, Francis; Hew, Mark; Rolland, Jennifer M.; O’Hehir, Robyn E; Zelm, Menno C. van

doi:10.1111/all.14832

Cited by 8 publications

(26 citation statements)

References 62 publications

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“…By virtue of polyclonal IgE binding with high‐affinity FcεRI, blood basophils can be a target cell for evaluation of allergen sensitization (Figures 2C and 3B). 83 A recent study from our group demonstrated nearly 100% positive predictive values for the detection of sensitization to bee venom and ryegrass pollen using fluorescent allergen tetramers 84 …”

Section: Future Prospectsmentioning

confidence: 98%

“…With clinical flowcytometers currently having 10–12 fluorescent parameters, multiplex detection is possible with 8–10 allergen components in combination with IgE and CD123 as basophil markers. Without the need for basophil activation, 85 cytometric detection of allergen sensitization on blood basophils (CytoBas) can be performed on fresh and frozen PBMC in a standard pathology laboratory 84 …”

Section: Future Prospectsmentioning

confidence: 99%

“…83 A recent study from our group demonstrated nearly 100% positive predictive values for the detection of sensitization to bee venom and ryegrass pollen using fluorescent allergen tetramers. 84 With clinical flowcytometers currently having 10-12 fluorescent parameters, multiplex detection is possible with 8-10 allergen components in combination with IgE and CD123 as basophil markers. Without the need for basophil activation, 85 cytometric detection of allergen sensitization on blood basophils (CytoBas) can be performed on fresh and frozen PBMC in a standard pathology laboratory.…”

Section: Futurepros Pec Tsmentioning

confidence: 99%

“…Without the need for basophil activation, 85 cytometric detection of allergen sensitization on blood basophils (CytoBas) can be performed on fresh and frozen PBMC in a standard pathology laboratory. 84 Targeted panels of allergen components would be more cost-effective than large-scale array technologies. Furthermore, serum IgG can easily be washed away from cells, thereby not interfering with detection as seen for serum IgE tests.…”

Section: Futurepros Pec Tsmentioning

confidence: 99%

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Advances in allergen‐specific immune cell measurements for improved detection of allergic sensitization and immunotherapy responses

Zelm

McKenzie

Varese

et al. 2021

Allergy

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Over the past two decades, precision medicine has advanced diagnostics and treatment of allergic diseases. Component-resolved analysis of allergen sensitization facilitates stratification of patients. Furthermore, new formulations of allergen immunotherapy (AIT) products can more effectively deliver the relevant components.Molecular insights from the identification of allergen component sensitization and clinical outcomes of treatment with new AIT formulations can now be utilized for a deeper understanding of the nature of the pathogenic immune response in allergy and how this can be corrected by AIT. Fundamental in these processes are the allergenspecific B and T cells. Within the large B-and T-cell compartments, only those that specifically recognize the allergen with their immunoglobulin (Ig) or T-cell receptor (TCR), respectively, are of clinical relevance. With peripheral blood allergen-specific B-and T-cell frequencies below 1%, bulk cell analysis is typically insufficiently sensitive. We here review the latest technologies to detect allergen-specific B and T cells, as well as new developments in utilizing these tools for diagnostics and therapy monitoring to advance precision medicine for allergic diseases.

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Section: Future Prospectsmentioning

confidence: 98%

Section: Future Prospectsmentioning

confidence: 99%

Section: Futurepros Pec Tsmentioning

confidence: 99%

Section: Futurepros Pec Tsmentioning

confidence: 99%

See 2 more Smart Citations

Advances in allergen‐specific immune cell measurements for improved detection of allergic sensitization and immunotherapy responses

Zelm

McKenzie

Varese

et al. 2021

Allergy

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“…Sustained unresponsiveness (SU) to peanut was observed in patients with low basophil activation at baseline and those with a greater than 80% reduction in peanut‐induced basophil activation after OIT, 22 supporting the utility of BAT in predicting and monitoring response to OIT. These new technologies including others using novel gating strategies with optimization of storage and automation of measurements and analysis may enable routine high‐throughput analysis in the future 19,23‐25 …”

Section: Diagnosis and Endotypingmentioning

confidence: 99%

Food allergy, mechanisms, diagnosis and treatment: Innovation through a multi‐targeted approach

Sindher

Long

Chin

et al. 2022

Allergy

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The incidence of food allergy (FA) has continued to rise over the last several decades, posing significant burdens on health and quality of life. Significant strides into the advancement of FA diagnosis, prevention, and treatment have been made in recent years. In an effort to lower reliance on resource‐intensive food challenges, the field has continued work toward the development of highly sensitive and specific assays capable of high‐throughput analysis to assist in the diagnosis FA. In looking toward early infancy as a critical period in the development of allergy or acquisition of tolerance, evidence has increasingly suggested that early intervention via the early introduction of food allergens and maintenance of skin barrier function may decrease the risk of FA. As such, large‐scale investigations are underway evaluating infant feeding and the impact of emollient and steroid use in infants with dry skin for the prevention of allergy. On the other end of the spectrum, the past few years have been witness to an explosive increase in clinical trials of novel and innovative therapeutic strategies aimed at the treatment of FA in those whom the disease has already manifested. A milestone in the field, 2020 marked the approval of the first drug, oral peanut allergen, for the indication of peanut allergy. With a foundation of promising data supporting the safety and efficacy of single‐ and multi‐allergen oral immunotherapy, current efforts have turned toward the use of probiotics, biologic agents, and modified allergens to optimize and improve upon existing paradigms. Through these advancements, the field hopes to gain footing in the ongoing battle against FA.

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Accurate determination of house dust mite sensitization in asthma and allergic rhinitis through cytometric detection of Der p 1 and Der p 2 binding on basophils (CytoBas)

Hsin,

Varese,

Aui

et al. 2024

Journal of Allergy and Clinical Immunology

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CytoBas: Precision component‐resolved diagnostics for allergy using flow cytometric staining of basophils with recombinant allergen tetramers

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

Cited by 8 publications

References 62 publications

Advances in allergen‐specific immune cell measurements for improved detection of allergic sensitization and immunotherapy responses

Advances in allergen‐specific immune cell measurements for improved detection of allergic sensitization and immunotherapy responses

Food allergy, mechanisms, diagnosis and treatment: Innovation through a multi‐targeted approach

Accurate determination of house dust mite sensitization in asthma and allergic rhinitis through cytometric detection of Der p 1 and Der p 2 binding on basophils (CytoBas)

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